中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (33): 6231-6234.doi: 10.3969/j.issn.1673-8225.2011.33.037

• 组织构建学术探讨 tissue construction academic discussion • 上一篇    下一篇

成骨细胞的体外培养与鉴定

王勇平，廖  燚，蒋  垚   

  1. 上海交通大学附属上海市第六人民医院骨科，上海市  200233
  • 收稿日期:2011-02-09 修回日期:2011-04-24 出版日期:2011-08-13 发布日期:2011-08-13
  • 通讯作者: 蒋垚，主任医师，教授，博士生导师，上海交通大学附属第六人民医院骨科，上海市 200233 jiangyao195106@163.com
  • 作者简介:王勇平☆，男，1975年生，甘肃省宁县人，汉族，上海交通大学在读博士，主治医师，主要从事关节外科，骨科生物材料的研究。 wangyp312@ 163.com

In vitro culture and identification of osteoblasts

Wang Yong-ping, Liao Yi, Jiang Yao   

  1. Department of Orthopedics, Sixth People’s Hospital of Shanghai Jiao Tong University, Shanghai  200233, China
  • Received:2011-02-09 Revised:2011-04-24 Online:2011-08-13 Published:2011-08-13
  • Contact: Jiang Yao, Chief physician, Professor, Doctoral supervisor, Department of Orthopedics, Sixth People’s Hospital of Shanghai Jiao Tong University, Shanghai 200233, China jiangyao195106@ 163.com
  • About author:Wang Yong-ping☆, Studying for doctorate, Attending physician, Department of Orthopedics, Sixth People’s Hospital of Shanghai Jiao Tong University, Shanghai 200233, China wangyp312@163. com

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187

被引次数

42

摘要：

背景：成骨细胞的体外培养方法均存在一定的局限性。
目的：对体外培养成骨细胞的来源，成骨细胞的培养方法及培养条件，鉴定成骨细胞的标志进行总结。
方法：以“Osteoblast, cell culture, identification”为检索词，检索PubMed数据库(2000/2010)，以“成骨细胞、细胞培养、鉴定”为检索词，检索万方数据库(2000/2010)，文献检索语种限制为英文和中文。纳入与成骨细胞的体外培养及鉴定密切相关的研究，排除重复性研究和Meta分析后对文献进行综述。
结果与结论：随着体外细胞培养技术的发展，人们已经从许多动物的骨、骨膜、骨髓及骨外组织中成功培养了成骨细胞，经鉴定具有典型成骨细胞的特征及良好的生物学特性，目前体外培养成骨细胞为常用的体外实验模型，成为研究骨生理、病理及修复的重要手段，也成为研究成骨细胞生长代谢及骨组织工程的基础。

关键词: 成骨细胞, 细胞培养, 鉴定, 生物学特性, 骨组织工程

Abstract:

BACKGROUND: The methods of osteoblast culture in vitro have certain limitations.
OBJECTIVE: To summarize osteoblast source, in vitro culture method, culture condition, and identification signs.
METHODS: A computer-based retrieval was performed in PubMed and Wanfang databases to search manuscripts published between 2000 and 2010 with the key words” osteoblast, cell culture, identification” in English and Chinese languages. The papers describing in vitro culture and identification of osteoblasts were included. Repetitive research and Meta analysis were excluded.
RESULTS AND CONCLUSION: With the development of cell culture techniques in vitro, osteoblasts from animal bone, periosteum, bone marrow and non-bone tissue have been successfully cultured. Studies have shown that these osteoblasts have typical characteristics and good biological properties. Currently, the cultured osteoblasts are common experimental model in vitro to study physiology, pathology, and repair and have become the basis for studying osteoblast growth and metabolism and bone tissue engineering.

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