中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (33): 6165-6167.doi: 10.3969/j.issn.1673-8225.2011.33.021

• 组织构建与生物活性因子 tissue construction and bioactive factors • 上一篇    下一篇

α-玉米赤霉醇干预骨质疏松性骨折模型大鼠白细胞介素1、6的表达

宗少晖1，韦  波2，曾高峰2，熊春翔3，赵玉玺3   

  1. 1广西医科大学第一附属医院脊柱骨病外科，广西壮族自治区南宁市  530021；广西医科大学，2公共卫生学院，3研究生学院，广西壮族自治区南宁市  530021
  • 收稿日期:2011-02-15 修回日期:2011-04-16 出版日期:2011-08-13 发布日期:2011-08-13
  • 作者简介:宗少晖☆，男，1972年生，广西壮族自治区人，瑶族，医学博士，副教授，主要从事骨质疏松症的临床及基础研究。 xiaohui3008@ 126.com
  • 基金资助:

    广西自然科学基金(桂科青0832041)，广西卫生厅科研基金(桂卫Z2007046)。

Effect of a-zearanol on interleukin-1, 6 expressions in a rat model of osteoporotic fracture

Zong Shao-hui1, Wei Bo2, Zeng Gao-feng2, Xiong Chun-xiang3, Zhao Yu-xi3   

  1. 1Department of Spine Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning  530021, Guangxi Zhuang Autonomous Region, China; 2School of Public Health, 3Postgraduate School, Guangxi Medical University, Nanning  530021, Guangxi Zhuang Autonomous Region, China
  • Received:2011-02-15 Revised:2011-04-16 Online:2011-08-13 Published:2011-08-13
  • About author:Zong Shao-hui☆, M.D., Associate professor, Department of Spine Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China xiaohui3008@126. com
  • Supported by:

    the Natural Science Foundation of Guangxi Zhuang Autonomous Region, No. Guikeqing0832041*; Scientific Research Foundation of Health Department of Guangxi i Zhuang Autonomous Region, No. GuiweiZ2007046*

PDF

415

摘要：

背景：α-玉米赤霉醇是一种新的植物雌激素，可能具有动物雌激素的某些生理功能。
目的：观察植物雌激素α-玉米赤霉醇对骨质疏松性骨折大鼠白细胞介素1和白细胞介素6表达的影响。
方法：切除40只6月龄雌性SD大鼠双侧卵巢建立去卵巢骨质疏松模型，造模成功后锯断右侧胫骨建立骨折模型，骨折1周，分别灌胃0.066 mg/kg雌二醇、10，5，1 mg/kg α-玉米赤霉醇及二甲基亚砜；另取10只大鼠，不切除卵巢，制备骨折模型后灌胃二甲基亚砜。给药35 d，ELISA法测定大鼠血清白细胞介素1和白细胞介素6的表达。
结果与结论：去卵巢后，骨折大鼠血清白细胞介素1和白细胞介素6的表达明显升高，5，1 mg/kg α-玉米赤霉醇及0.066 mg/kg雌二醇均可明显降低骨质疏松性骨折大鼠血清白细胞介素1和白细胞介素6的表达，但10 mg/kg α-玉米赤霉醇作用效果不显著。说明α-玉米赤霉醇可以剂量依赖性地降低骨质疏松性骨折大鼠血清白细胞介素1和白细胞介素6的表达。

关键词: 骨质疏松性骨折, a-玉米赤霉醇, 去卵巢, 白细胞介素1, 白细胞介素6

Abstract:

BACKGROUND: a-zearanol (α-ZAL) is a new phytoestrogen that may have some physiological functions of animal estrogen.
OBJECTIVE: To investigate the effects of α-ZAL on interleukin (IL)-1and IL-6 in rats with osteoporotic fracture.
METHODS: Osteoporosis was induced in 40 female 6-week-old Sprague-Dawley rats by bilateral ovariectomy. Thereafter, the right tibia was cut off to establish rat models of fracture. One week later, rats were intragastrically administered 0.066 mg/kg estradiol, or 10, 5, 1 mg/kg α-ZAL and dimethyl sulfoxide; another 10 rats did not underwent ovariectomy, but developed fracture and received intragastric administration of dimethyl sulfoxide. After administration for 35 days, serum level of IL-1 and IL-6 was determined by ELISA.
RESULTS AND CONCLUSION: After ovariectomy, serum level of IL-1 and IL-6 was obviously increased in fracture rats. 5,  1 mg/kg α-ZAL and 0.066 mg/kg estradiol could markedly decrease serum level of IL-1 and IL-6 in osteoporotic fracture rats, but   10 mg/kg α-ZAL did not produce obvious effects. The results showed that α-ZAL could decrease serum level of IL-1 and IL-6 in osteoporotic fracture rats in a dose-dependent manner.

中图分类号: