中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (29): 5371-5374.doi: 10.3969/j.issn.1673-8225.2011.29.013

• 纳米生物材料 nanobiomaterials • 上一篇    下一篇

纳米Ag-SiO2导尿管的细胞毒性评价

翟军红1，郭云童1，刘  春1，张  利1，张雁钢1，张  进2   

  1. 1山西医科大学第一医院，山西省太原市   030001
    2山西医科大学，山西省太原市   030001
  • 收稿日期:2010-12-06 修回日期:2011-02-20 出版日期:2011-07-16 发布日期:2011-07-16
  • 通讯作者: 刘春，主任医师，硕士生导师，山西医科大学第一医院，山西省太原市 030001 sxtyliuchun@126.com
  • 作者简介:翟军红★，男，1982年生，山西省长治市人，汉族，2010年山西医科大学毕业，硕士，主要从事泌尿系感染的研究。
  • 基金资助:

    山西省自然科学基金资助项目（2006011119）

Cytotoxicity evaluation of nano-Ag-SiO2 urethral catheter

Zhai Jun-hong1, Guo Yun-tong1, Liu Chun1, Zhang Li1, Zhang Yan-gang1, Zhang Jin2   

  1. 1First Hospital of Shanxi Medical University, Taiyuan  030001, Shanxi Province, China
    2Shanxi Medical University, Taiyuan  030001, Shanxi Province, China
  • Received:2010-12-06 Revised:2011-02-20 Online:2011-07-16 Published:2011-07-16
  • Contact: Liu Chun, Chief physician, Master’s supervior, First Hospital of Shanxi Medical University, Taiyuan 030001, Shanxi Province, China sxtyliuchun@126.com
  • About author:Zhai Jun-hong★, Master, First Hospital of Shanxi Medical University, Taiyuan 030001, Shanxi Province, China
  • Supported by:

    the Natural Science Foundation of Shanxi Province, No. 2006011119*

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摘要：

背景：具有抗菌活性的纳米Ag-SiO2导尿管可有效降低普通医用导尿管相关性尿路感染的发生率，其物理、化学性质发生很大变化，所以对其安全性需重新评价。
目的：比较纳米Ag-SiO2导尿管和普通医用导尿管浸提液对兔尿道上皮细胞的体外细胞活性影响，初步评价纳米Ag-SiO2导尿管的生物安全性。
方法：应用机械分离与酶消化法分离培养兔尿道上皮细胞,在体外行扩增后制成细胞悬液，将培养液更换为浸泡纳米Ag-SiO2导尿管及普通导尿管的培养液，采取四甲基偶氮唑盐比色法量化两组浸提液对兔尿道上皮细胞的体外细胞毒性。用酶联免疫检测仪分别测定两组体外细胞的吸光度值，并计算其相对增殖率(RGR)，进行毒性评价及比较。
结果与结论：纳米Ag-SiO2导尿管和普通医用导尿管均毒性轻微。纳米Ag-SiO2导尿管与普通医用导尿管相对增殖率均数间差别无显著性意义(P > 0.05)。结果表明，纳米Ag-SiO2导尿管对细胞的生长繁殖同普通医用导尿管一样影响轻微，无(或)低细胞毒性，符合医疗器械生物学评价标准要求。

关键词: 纳米Ag-SiO2, 尿道上皮细胞, 导尿管, 四甲基偶氮唑盐, 安全性

Abstract:

BACKGROUND: Antibacterial activated nano- Ag-SiO2 urethral catheter can reduce the incidence of catheter-associated urinary tract infections obviously. Because of diversification of physical and chemical properties, security shoud be re-evaluated.
OBJECTIVE: Based on comparison of nano-Ag-SiO2 urethral catheter and ordinary urethral catheter extracts on in vitro activity of rabbit epithelial cells, to perform a preliminary evaluation of nano-Ag-SiO2 urethral catheter biological safety.
METHODS: Mechanical separation and enzymatic digestion were used to isolate and culture rabbit urothelial cells to make cell suspension after in vitro proliferation. The medium was replaced by immersion nano-Ag-SiO2 and general catheter tube medium. MTT assay was performed to quantify two extracts and detect in vitro cytotoxicity of rabbit urethra epithelial cells. Absorbance values of nano-Ag-SiO2 and general urinary catheter groups were measured by enzyme-linked immunosorbent assay to calculate the relative growth rate, and compare and perform toxicity evaluation.
RESULTS AND CONCLUSION: Nano-Ag-SiO2 and general anti-infective urinary catheter were slightly toxic. The relative growth rate had no difference between the two groups (P > 0.05). Nano-Ag-SiO2 urethral catheter effect on cell growth and reproduction is same as that of general urinary catheter, and there is no or low cytotoxicity that is consistent with the biological evaluation standards of medical devices.

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