中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (15): 2669-2671.doi: 10.3969/j.issn.1673-8225.2011.15.003

• 骨组织构建 bone tissue construction • 上一篇    下一篇

血管内皮细胞生长因子与半月板白区修复中新生血管的长入

孙延山1，卢  斌2，姜  鑫1，张益民1，郭永智1，王  军1，刘建永1，李  伟1，曹学军1   

  1. 1潍坊市人民医院关节外科，山东省潍坊市  261041
    2昌邑市人民医院骨科，山东省昌邑市  261300
  • 收稿日期:2010-10-06 修回日期:2010-11-17 出版日期:2011-04-09 发布日期:2013-11-06
  • 作者简介:孙延山★，男，1976年，山东省潍坊市，汉族，2003年浙江大学毕业，硕士，主治医师，主要从事关节外科的研究。 qwertyui698@sohu.com

Effect of vascular endothelial growth factor on neovascularization growth into white area of injured meniscus

Sun Yan-shan1, Lu Bin2, Jiang Xin1, Zhang Yi-min1, Guo Yong-zhi1, Wang Jun1, Liu Jian-yong1, Li Wei1, Cao Xue-jun1   

  1. 1Department of Joint Surgery, Weifang People’s Hospital, Weifang  261041, Shandong Province, China
    2Department of Orthopaedics, the People’s Hospital of Changyi, Changyi  261300, Shandong Province, China
  • Received:2010-10-06 Revised:2010-11-17 Online:2011-04-09 Published:2013-11-06
  • About author:Sun Yan-shan★, Master, Attending physician, Department of Joint Surgery, Weifang People’s Hospital, Weifang 261041, Shandong Province, China qwertyui698@sohu.com

PDF

437

被引次数

8

摘要：

背景：血管内皮细胞生长因子的研究集中于对皮肤坏死、肌腱的修复作用，对半月板的影响研究甚少。
目的：观察血管内皮细胞生长因子能否促进血管向损伤半月板的白区生长及半月板白区愈合。
方法：将新西兰大白兔24只随机选择左后肢或右后肢作为实验组，另一条后肢作为对照组，均建立半月板后角损伤模型，实验组关节内注射血管内皮细胞生长因子。术后1，3，6，12周切除半月板，制作切片进行大体和组织学观察。
结果与结论：术后1周，实验组较对照组血管增生，成纤维细胞丰富，并且有透明软骨细胞出现。术后3周，实验组的软骨层明显较对照组厚。术后12周，实验组软骨层出现成骨基质和成骨细胞。结果提示术后早期，血管内皮细胞生长因子促进了血管的发生，从而促进损伤区的修复；但晚期随血管的长入，促进了软骨内化骨，损伤了已修复的软骨层。

关键词: 血管内皮细胞生长因子, 半月板, 白区损伤, 修复, 血管增生

Abstract:

BACKGROUND: The protective mechanism of vascular endothelial growth factor (VEGF) is focus on cellular necrosis of skin and ligament reparation, studies regarding meniscus are few.
OBJECTIVE: To explore whether VEGF could induce vascular endothelial cell proliferation into white area of injured meniscus, and promote healing of meniscus.
METHODS: Totally 24 New Zealand white rabbits were prepared for hind limb injured meniscus posterior horn models and randomly divided into the experimental group and control group. The experimental group received intra-articular injection of VEGF. The gross and histological observations of meniscus were performed at 1, 3, 6, and 12 weeks. 
RESULTS AND CONCLUSION: Compared to control group, there have more proliferated vascular, fibroblast, and has appeared hyaline cartilage cells in Experimental group in the first week after operation. The cartilage layer of experimental is group thicker than that of the control group in 3 weeks after operation; the experimental group has cartilage layer and osteoblast bone matrix in 12 weeks after operation. Vascular endothelial growth factor promotes the occurrence of blood vessels, thereby promoting repair of injured area in early postoperative period; however, the ingrowth of blood vessels promotes endochondral bone and damages repaired cartilage layer at late stage.

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