中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (8): 1336-1340.doi: 10.3969/j.issn.1673-8225.2011.08.002

• 复合支架材料 composite scaffold materials • 上一篇    下一篇

生物可降解聚合物结合碱性成纤维细胞生长因子与内皮细胞的黏附

武  欣1，谷涌泉1，张  建1，叶  霖2，段红永1，吴英峰1，陈  兵1，李建新1，张淑文1，冯增国2，汪忠镐1   

  1. 1首都医科大学宣武医院血管外科，北京市100053
    2北京理工大学材料学院，北京市    100081
  • 收稿日期:2010-08-25 修回日期:2010-10-07 出版日期:2011-02-19 发布日期:2011-02-19
  • 通讯作者: 谷涌泉，博士，主任医师，首都医科大学宣武医院血管外科，北京市 100053 gu-yq@263.net
  • 作者简介:武欣☆，男，1975年生，河北省石家庄市人，汉族，2010年首都医科大学毕业，博士，主治医师，主要从事血管外科基础与临床研究。 wuxinbj@yahoo.cn
  • 基金资助:

    国家高技术研究发展计划(863计划)资助项目：“小口径组织工程血管的基础及应用研究”(2006AA02A134)；北京市自然科学基金资助项目：多孔脱细胞小口径组织工程动脉支架及其再细胞化研究”(5082007)和“在体靶向自组装小口径组织工程动脉的初步研究”(2103049)。

Adhesion of endothelial cells with biodegradable polymer and basic fibroblast growth factor

Wu Xin1, Gu Yong-quan1, Zhang Jian1, Ye Lin2, Duan Hong-yong1, Wu Ying-feng1, Chen Bing1, Li Jian-xin1, Zhang Shu-wen1, Feng Zeng-guo2, Wang Zhong-gao1   

  1. 1Department of Vascular Surgery, Xuanwu Hospital, Capital Medical University, Beijing  100053, China
    2School of Material Science, Beijing Institute of Technology, Beijing  100081, China
  • Received:2010-08-25 Revised:2010-10-07 Online:2011-02-19 Published:2011-02-19
  • Contact: Gu Yong-quan, Doctor, Chief physician, Department of Vascular Surgery, Xuanwu Hospital, Capital Medical University, Beijing 100053, China gu-yq@263.net
  • About author:Wu Xin☆, Doctor, Attending physician, Department of Vascular Surgery, Xuanwu Hospital, Capital Medical University, Beijing 100053, China wuxinbj@yahoo.cn
  • Supported by:

    the National High-Tech Research and Development Program of China (863 Program), No. 2006AA02A134*; Beijing Natural Science Foundation, No. 5082007*, 2103049*

PDF

411

被引次数

4

摘要：

背景：可降解材料的应用是体外构建小口径组织工程血管的重要研究部分。如何对可降解材料进行改性，以利于材料本身体外抗凝与促进内皮细胞黏附，是目前血管组织工程研究的热点之一。
目的：利用可降解聚己内酯接枝肝素材料体外负载碱性成纤维细胞生长因子，观察其对于内皮细胞黏附的影响。
方法：应用聚己内酯可降解材料，将肝素活化后并与聚己内酯的端羟基发生酯化反应从而被锚定在聚己内酯两端。经过电纺丝技术，制备血管支架。同时利用肝素和生长因子间的静电吸附作用，使支架负载碱性成纤维细胞生长因子。采用低密度内皮细胞短期静态种植，观察负载细胞生长因子的可降解聚己内酯材料对内皮细胞生长黏附情况的影响。
结果与结论：成功地制备了负载成纤维细胞生长因子的可降解聚己内酯接枝肝素支架。内皮黏附实验证实，该支架利于内皮细胞黏附。提示可降解聚己内酯接枝肝素材料负荷碱性细胞生长因子支架对内皮细胞有很好的体外黏附性。

关键词: 可降解, 聚已内酯, 肝素, 支架, 内皮细胞, 碱性成纤维细胞生长因子, 组织工程

Abstract:

BACKGROUND: The application of biodegradable materials is the important research component of small-caliber tissue engineered vessels in vitro. How to modify degradable materials so as to facilitate in vitro anticoagulant and promote endothelial cell adhesion, is one of the hotspots in the study of vascular tissue engineering. 
OBJECTIVE: To in vitro load basic fibroblast growth factor (bFGF) by using biodegradable polycaprolactone (PCL) graft heparin materials, and to observe the effects on endothelial cell adhesion.
METHODS: Applying PCL biodegradable materials, the activated heparin esterified with PCL hydroxyl end and then anchored at both ends of the PCL. The vascular scaffold was prepared with electrospinning technique. Using the electrostatic adsorption between heparin and growth factors, the scaffold was loaded with bFGF. After low-density short-term static cultivation of endothelial cells, the bFGF-loaded biodegradable PCL on the adhesion of endothelial cells was investigated.
RESULTS AND CONCLUSION: The bFGF-loaded biodegradable PCL graft heparin scaffold is successfully fabricated. Endothelial adhesion experiments demonstrated that the biodegradable scaffold was conducive to endothelial cell adhesion. Biodegradable PCL graft heparin material loading bFGF is beneficial to endothelial cell adhesion in vitro.

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