中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (7): 1219-1223.doi: 10.3969/j.issn.1673-8225.2011.07.017

• 组织构建细胞学实验 cytology experiments in tissue construction • 上一篇    下一篇

低强度脉冲超声波对Beagle犬Ⅱ度根分叉病变的辅助效应

何  平1，高  翔1，宋锦璘1，邓  锋1，赵纯亮2，王智彪2，李  娜3   

  1. 1重庆医科大学附属口腔医院正畸科，重庆市 400015
    2重庆超声医学工程重庆市市级重点实验室，重庆市400016
    3川北医学院附属医院口腔科，四川省南充市 637000
  • 收稿日期:2010-11-29 修回日期:2011-01-13 出版日期:2011-02-12 发布日期:2011-02-12
  • 通讯作者: 宋锦璘，博士，主任医师，教授，硕士生导师，重庆医科大学附属口腔医院正畸科，重庆市400015 soongjl@163. com
  • 作者简介:何平★，男，1978年生，四川省达州市人，汉族，重庆医科大学在读硕士，主治医师，主要从事口腔临床医学相关研究。 hping78@gmail.com
  • 基金资助:

    国家自然科学基金(30870754)；重庆市发展和改革委员会高技术产业重大产业技术开发项目(渝发改技 [2007]1110号)；重庆卫生局科研项目(2008-2-254)。 

Secondary repair effects of low-intensity pulsed ultrasound on class Ⅱ furcation involvement in Beagle dogs

He Ping1, Gao Xiang1, Song Jin-lin1, Deng Feng1, Zhao Chun-liang2, Wang Zhi-biao2, Li Na3     

  1. 1Department of Orthodontics, College of Stomatology, Chongqing Medical University, Chongqing  400015, China
    2Chongqing Key Laboratory of Ultrasound Medical Engineering, Chongqing  400016, China
    3Department of Stomotology of the Affiliated Hospital of North Sichuan Medical College, Nanchong  637000, Sichuan Province, China
  • Received:2010-11-29 Revised:2011-01-13 Online:2011-02-12 Published:2011-02-12
  • Contact: Song Jin-lin, Doctor, Chief physician, Professor, Master’s supervisor, Department of Orthodontics, College of Stomatology, Chongqing Medical University, Chongqing 400015, China soongjl@163.com
  • About author:He Ping★, Studying for master’s degree, Attending physician, Department of Orthodontics, College of Stomatology, Chongqing Medical University, Chongqing 400015, China hping78@gmail.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30870754*; the Major High-tech Industry Development Projects in Industrial Technology of Chongqing  Municipal Development and Reform Commission, Chongqing Development and Reform Technology  No. [2007]1110*; Research Projects of Chongqing Municipal Health Bureau, No. 2008-2-254*

PDF

568

被引次数

20

摘要：

背景：低强度脉冲超声波主要用于促进骨折愈合及提高新生骨组织密度，尚未见其对Ⅱ度根分叉病变组织学修复效应的报道。
目的：观察低强度脉冲超声波对Ⅱ度根分叉病变的组织学修复效应。
方法：5只Beagle犬各取2颗下颌第4前磨牙颊侧作为实验区，建立Ⅱ度根分叉病变模型，分别分为超声组和对照组并在颊侧缺损龈方根面制备切迹作为参照点，高糖饲养8周。超声组行根面平整术，术后1周行低强度脉冲超声波(90 mW/cm2，1.5 MHz，200 μs，1 kHz)处理20 min/d，对照组行根面平整术和假处理。6周后取实验区域组织制备脱钙切片，苏木精-伊红染色、Masson染色行根分叉区域组织测量分析。
结果与结论：超声组根分叉区新生牙槽骨沿根面生长，根分叉区可见牙龈上皮；对照组根分叉区以结缔组织生长为主，可见大量牙龈上皮和少量新生骨组织。超声组新生牙槽骨胶原呈“红-蓝”相间，以红染为主；对照组呈现蓝染，即超声组的新生牙槽骨胶原较对照组成熟。超声组牙槽骨、牙骨质、牙周膜新生量均大于对照组(P < 0.05)。提示90 mW/cm2低强度脉冲超声波可促进根分叉病变新生牙槽骨胶原成熟和改建。

关键词: 低强度脉冲超声波, Beagle犬, Ⅱ度根分叉病变, 胶原, 修复

Abstract:

BACKGROUND: There were many reports on low-intensity pulsed ultrasound (LIPUS) to promote fracture healing and increase bone density, there were not literature involved in tissue repair effects of LIPUS on class Ⅱ furcation involvement.
OBJECTIVE: To investigate the tissue repair effects of LIPUS treatment on class Ⅱ furcation involvement.
METHOD: Beagle dogs’ class Ⅱ furcation involvement models were established at buccal region of the dogs’ fourth premolar of bilateral mandibular and randomly divided into ultrasonic treatment group (UT group) and control group (C group) and set notch at the gingival side of buccal root surface as the reference point. Then, Beagle dogs were fed with high glucose for 8 weeks. Scaling and planning of the root surface was applied on UT group and 1 week later take LIPUS (90 mW/cm2, 1.5 MHz, 200 μs,   1 kHz) to irradiate UT group by 20 minutes per day, while scaling and planning of the root surface and sham irradiation were applied on C group. After 6 weeks, decalcified tissue sections of hematoxylin-eosin staining and Masson staining were used to measure and analysis the furcation tissues.
RESULTS AND CONCLUSION: In the UT group, new alveolar bone of furcation area grew along the root surface and gingival epithelium appeared; in the C group, it mainly changed by connective tissue growth at furcation area as well as a large number of gingival epithelium and a small amount of new alveolar bone. The color of new alveolar bone collagen in the UT group was between red and blue, the color of new alveolar bone collagen in the C group showed blue, which meant the new alveolar bone collagen in the UT group was more mature than it in the C group. The new volumes of alveolar bone, cementum and periodontal ligament in the UT group were more than those in the C group (P < 0.05). So 90 mW/cm2 LIPUS can promote new bone collagen maturation and remodeling at furcation area.

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