中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (50): 9387-.doi: 10.3969/j.issn.1673-8225.2010.50.019

• 组织构建与中医药 • 上一篇    下一篇

揉髌手法对兔膝关节软骨细胞凋亡及增殖细胞核抗原表达的影响

欧国建1，戴七一2，覃学流2，林光琪3，刘靖3，滕居赞2，姚平2，朱江龙2，容向宾2，韩杰2，辛晓东3，袁经阳3   

  1. 1广西中医学院附属贺州医院骨伤科，广西壮族自治区贺州市 542500；2广西中医学院附属瑞康医院骨四肢关节科，广西壮族自治区南宁市 530011；3广西中医学院，广西壮族自治区南宁市 530001
  • 出版日期:2010-12-10 发布日期:2010-12-10
  • 通讯作者: 戴七一，硕士，教授，广西中医学院附属瑞康医院骨四肢关节科，广西壮族自治区南宁市530011 DIQY3146423@163.com 覃学流，副教授，广西中医学院附属瑞康医院骨四肢关节科，广西壮族自治区南宁市 530011 qinxueliu 2008@163.com
  • 作者简介:欧国建★，男，1981年生，四川省资阳市人，广西中医学院骨伤专业在读硕士，医师。主要从事骨科疾病的治疗工作。 ogj321@163.com
  • 基金资助:

    课题受国家自然科学基金项目(30860359)、广西区科技厅自然科学基金项目(0832178)资助。

Effects of roubin-manipulation on knee joint chondrocyte apoptosis and proliferating cell nuclear antigen expression in rabbits

Ou Guo-jian1, Dai Qi-yi2, Qin Xue-liu2, Lin Guang-qi3, Liu Jing3, Teng Ju-zan2, Yao Ping2, Zhu Jiang-long2, Rong Xiang-bin2, Han Jie2, Xin Xiao-dong3, Yuan Jing-yang3   

  1. 1 Department of Orthopaedic Traumatology, Hezhou Hospital, Guangxi Traditional Chinese Medicine College, Hezhou  542500, Guangxi Zhuang Autonomous Region, China; 2 Department of Orthopedics, Ruikang Hospital, Guangxi Traditional Chinese Medicine College, Nanning  530011, Guangxi Zhuang Autonomous Region, China; 3 Guangxi Traditional Chinese Medicine College, Nanning  530001, Guangxi Zhuang Autonomous Region, China
  • Online:2010-12-10 Published:2010-12-10
  • Contact: Dai Qi-yi, Master, Professor, Department of Orthopedics, Ruikang Hospital, Guangxi Traditional Chinese Medicine College, Nanning 530011, Guangxi Zhuang Autonomous Region, China DIQY3146423@163.com Qin Xue-liu, Associate professor, Department of Orthopedics, Ruikang Hospital, Guangxi Traditional Chinese Medicine College, Nanning 530011, Guangxi Zhuang Autonomous Region, China qinxueliu 2008@163.com
  • About author:Ou Guo-jian★, Studying for master’s degree, Physician, Department of Orthopaedic Traumatology, Hezhou Hospital, Guangxi Traditional Chinese Medicine College, Hezhou 542500, Guangxi Zhuang Autonomous Region, China ogj321@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30860359*; the Natural Science Foundation of Science and Technology Commission of Guangxi, No. 0832178*

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摘要：

背景：有研究表明手法的力学刺激作用和关节被动活动可促进促进关节周围组织的自我修复，但手法治疗对膝关节软骨细胞凋亡和增殖的影响目前未见报道。
目的：用揉髌手法施术于骨内高压型动物的右下肢，探讨对兔膝关节软骨细胞凋亡和增殖细胞核抗原表达的影响。
方法：将新西兰兔随机分为5组：揉髌手法组、玻璃酸钠组和模型组采用手术结扎股静脉，臀下静脉、大隐静脉方法建立右下肢骨内高压型模型；假手术组予以手术暴露但不结扎相应血管；正常对照组不作任何处理。造模1周后，揉髌手法组采用揉髌手法连续治疗5周共17次；玻璃酸钠组每周右膝关节内注射1次，连续5周。于造模后12周末分别取所有动物右膝胫骨平台软骨组织，采用原位末端标记法观察软骨细胞凋亡，免疫组化法观察增殖细胞核抗原的表达。
结果与结论：揉髌手法组软骨细胞凋亡率低于模型组(P < 0.01)。揉髌手法组增殖细胞核抗原表达率高于模型组(P < 0.01)。结果表明揉髌手法治疗可以降低实验兔膝关节软骨细胞凋亡率及提高增殖细胞核抗原表达率。

 

关键词: 骨性关节炎, 揉髌手法, 关节软骨, 细胞凋亡, 增殖细胞核抗原, 组织构建

Abstract:

BACKGROUND: Studies have demonstrated that manipulation and joint passive movement can promote self-repair of surrounding tissues. However, it is poorly understood whether manipulation has effect on knee joint chondrocyte apoptosis and proliferating cell nuclear antigen expression (PCNA).
OBJECTIVE: To observe the effect of roubin-manipulation on knee joint chondrocyte apoptosis and PCNA expression.
METHODS: New Zealand rabbits were randomly divided into 5 groups: manipulation group, sodium hyaluronate group and model contrast group were established high intraosseous pressure models of right leg by ligating the vena femoralis, gluteae inferiors or great saphenous vein; sham surgery group only cut out the skin and the normal group. One week later, the manipulation group was treated with roubin-manipulation for 5 weeks with 17 times. The sodium hyaluronate was injected into the intra-articular of sodium hyaluronate group once a week for 5 weeks. Cartilage sections of right knee-joint were analyzed by in situ end labeling and immunohistochemistry at the end of 12 weeks.
RESULTS AND CONCLUSION: Compared with the model group, the apoptosis cell index of the manipulation group was smaller (P < 0.01) but the PCNA expression was greater (P < 0.01). The results demonstrated that roubin-manipulation can decrease the apoptosis of chondrocyte and increase chondrocyte proliferation. 

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