中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (49): 9290-.doi: 10.3969/j.issn.1673-8225.2010.49.039

• 干细胞学术探讨 • 上一篇    下一篇

脂肪源性干细胞生物学特性及在组织工程中的应用

魏茂平1，刘玉梅2，张自强2   

  1. 1内蒙古通辽市铁路医院外科门诊，内蒙古自治区通辽市 028000；  2河南科技大学动物科技学院，河南省洛阳市 471003
  • 出版日期:2010-12-03 发布日期:2010-12-03
  • 作者简介:魏茂平，男， 1961年生，内蒙古自治区通辽市人，汉族，1985年内蒙古民族大学毕业，主治医师，主要从事普外科方面研究。 Weimaoping12@126.com

Biological characteristics and application of adipose tissue-derived stem cells in tissue engineering 

Wei Mao-ping1, Liu Yu-mei2, Zhang Zi-qiang2   

  1. 1Outpatient Surgery, Inner Mongolia Tongliao Railway Hospital, Tongliao  028000, Inner Mongolia Autonomous Region, China; 2College of Animal Science and Technology, Henan University of Science and Technology, Luoyang  471003, Henan Province, China
  • Online:2010-12-03 Published:2010-12-03
  • About author:Wei Mao-ping, Attending physician, Outpatient Surgery, Inner Mongolia Tongliao Railway Hospital, Tongliao 028000, Inner Mongolia Autonomous Region, China Weimaoping12@126.com

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290

被引次数

1

摘要：

背景：脂肪源性干细胞具有自我更新及多向分化潜能，在体外适当的诱导条件下可向脂肪细胞、成骨细胞、软骨细胞、心肌细胞、神经细胞和肝细胞等多种细胞分化，在组织工程中具有良好的应用前景。
目的：了解脂肪源性干细胞的生物学特征及其在组织工程中的应用。
方法：以“tissue-derived stem cells，tissue engineering，脂肪，间充质干细胞，组织工程”为关键词检索Elsevier数据库2000-01/2010-05与中国期刊全文数据库2000-01/2010-05相关文章。
结果与结论：脂肪源性干细胞增殖速度快，取材方便、材料来源广，并且能自体取材，避免了免疫排斥问题。目前尚未找到鉴定脂肪源性干细胞的金标准，但研究者采用流式细胞仪和免疫组织化学方法研究发现体外培养的脂肪源性干细胞具有间充质干细胞这一类细胞的特异性表面标记。脂肪源性干细胞可向脂肪、骨、软骨、肌肉、造血、肝和神经等多种细胞分化。组织或器官缺损性疾病、退行性疾病及遗传性疾病可尝试通过组织工程技术将组织来源的干细胞与支架材料复合移植入体内，来解决这一临床难题已成为研究热点。

关键词: 脂肪源性干细胞, 生物学特性, 干细胞, 组织工程, 综述文献

Abstract:

BACKGROUND: Adipose tissue-derived stem cells possess self-renewal and multi-directional differentiation potential. In vitro it could be induced to different cells in specific differentiation medium, such as lipocytes, skeltogenous cells, cartilage cells, cardiac muscle cells, nerve cells and liver cells. These characteristics show that adipose tissue-derived stem cells possess favorable application prospect. 
OBJECTIVE: To understand the biological characteristics of adipose-derived mesenchymal stem cells, and to explore its clinical application in tissue engineering field.
METHODS: The databases of Elsevier (2000-01/2010-05) and China National Knowledge Infrastructure (2000-01/2010-05) were searched with key words of “adipose tissue-derived stem cell, tissue engineering”. Literature search was limited to English and Chinese languages.
RESULTS AND CONCLUSION: Adipose-derived stem cells could proliferate rapidly, and material was convenient to harvest. It could be obtained by itself, and also avoid immunological rejection. At present, there was no gold standard to identify adipose-derived stem cells, but investigators had observed in vitro cultured adipose-derived stem cells that had the specific surface labeling of mesenchymal stem cells using flow cytometry and immunohistochemical method. Adipose-derived stem cells could differentiate into adipocytes, osteocytes, chondrocytes, muscle cells, hematopoietic cells, liver cells and nerve cells. Tissue or organ defect disease, degenerative disease and hereditary disease can use tissue engineering technique to implant tissue-derived stem cells and stents into the bodies, which has been a research focus in clinic.

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