中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (49): 9272-.doi: 10.3969/j.issn.1673-8225.2010.49.035

• 干细胞学术探讨 • 上一篇    下一篇

骨髓间充质干细胞的成骨分化及临床应用

陈广东，杨惠林，王根林，汪李军   

  1. 苏州大学附属第一医院骨外科，江苏省苏州市 215006
  • 出版日期:2010-12-03 发布日期:2010-12-03
  • 通讯作者: 杨惠林，教授，博士生导师，苏州大学附属第一医院骨外科，江苏省苏州市 215006
  • 作者简介:陈广东★，男，1985年生，江苏省响水县人，汉族，苏州大学在读硕士，主要从事脊柱外科的研究。 cgdong0320502002@sina.com

Osteogenic differentiation and clinical application of bone marrow mesenchymal stem cells

Chen Guang-dong, Yang Hui-lin, Wang Gen-lin, Wang Li-jun   

  1. Department of Orthopaedic Surgery, First Affiliated Hospital, Soochow University, Suzhou  215006, Jiangsu Province, China
  • Online:2010-12-03 Published:2010-12-03
  • Contact: Yang Hui-lin, Doctoral supervisor, Professor, Department of Orthopaedic Surgery, First Affiliated Hospital, Soochow University, Suzhou 215006, Jiangsu Province, China
  • About author:Chen Guang-dong★, Studying for master’s degree, Department of Orthopaedic Surgery, First Affiliated Hospital, Soochow University, Suzhou 215006, Jiangsu Province, China cgdong0320502002@sina.com

PDF

426

被引次数

22

摘要：

背景：骨髓间充质干细胞具有良好的成骨分化潜能，在骨组织工程领域具有广泛的应用前景。然而，随着应用增多，存在问题日益突出，如分离纯化、最佳成骨分化浓度、致瘤性及安全性等问题还有待进一步解决。
目的：骨髓间充质干细胞的基础研究和临床应用广泛，但同时存在较多问题，就骨髓间充质干细胞的生物学特性、诱导分化、分离鉴定和临床应用进展进行综述。
方法：分别以“骨髓间充质干细胞, 成骨细胞”，“mesenchymal stem cells, osteoblast cells”为检索词，应用计算机检索中国期刊全文数据库及Pubmed 数据库和Ovid数据库1997/2010有关文章。纳入有关骨髓间充质干细胞的文献。排除内容重复和缺乏原创性文献。保留31篇文献做进一步分析。
结果与结论：目前从骨髓中分离骨髓间充质干细胞的方法主要为密度梯度离心法、免疫学分离法和贴壁筛选法。骨髓间充质干细胞的成骨定向诱导分化的主要技术特点是把具有成骨作用的基因转入靶细胞，由靶细胞转录成mRNA于病变区翻译成具有成骨作用的蛋白质，通过靶细胞的持续作用，促进自身成骨。许多基础研究表明依附于生物支架材料的骨髓间充质干细胞在适当的生长因子诱导下可以快速地向成骨细胞分化和增殖。已有很多骨不连和骨代谢障碍疾病患者受益于骨髓间充质干细胞的临床应用。

关键词: 骨髓间充质细胞, 成骨细胞, 骨组织工程, 诱导分化, 综述文献

Abstract:

BACKGROUND: Bone marrow mesenchymal stem cells (BMSCs) have potential to differentiate into osteoblasts and have great prospect in application as new source of seed cells in tissue engineering. However, there are still some problems such as isolation and purification of BMSCs, optimal density for differentiation into osteoblasts, oncogenicity and safety in vivo, deserving more attention and further investigation.
OBJECTIVE: Basic research and clinical application of BMSCs are extensive. There are still lots of problems deserving more concern. This study reviewed characteristics of biology, the induced differentiation, isolation and identification of BMSCs, and clinical applicability of BMSCs.
METHODS: We retrieved the articles in Chinese Journal Full-Text Database, PubMed database and Ovid database published from 1997 to 2010. Key words were “mesenchymal stem cells, osteoblast cells”. Articles concerning BMSCs were included, and literatures of repetitive content and lack of originality were excluded. A total of 31 literatures were obtained for further analysis.
RESULTS AND CONCLUSION: BMSCs were isolated from bone marrow mainly using density gradient centrifugation, immunology separation and adherence screening method at present. The main technical characteristics of differentiation of BMSCs into osteoblasts are to transfer gene with osteogenic effect into target cells; transcription into mRNA using target cells and translation into protein with osteogenic effects in the lesion site; oneself ossification was promoted through persistent effects of target cells. BMSCs depended on biological stents can rapidly differentiate into osteoblasts and proliferate under suitable induction of growth factors. Patients who suffered from fracture nonunion and metabolic bone diseases have benefited from clinical application of BMSCs.

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