中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (44): 8257-8260.doi: 10.3969/j.issn.1673-8225.2010.44.022

• 器官移植基础实验 basic experiments of organ transplantation • 上一篇    下一篇

部分肝切除大鼠不同时期肝再生组织中转化生长因子β1的表达

宋海岩1，高志涛2，刘恒兴1   

  1. 新乡医学院，1人体解剖学教研室，2免疫学研究中心，河南省新乡市 453003
  • 出版日期:2010-10-29 发布日期:2010-10-29
  • 通讯作者: 刘恒兴，硕士，教授，新乡医学院人体解剖学教研室，河南省新乡市 453003 liuhengxing@hotmail.com
  • 作者简介:宋海岩★，女，1980年生，河南省新乡市人，汉族，2007年山东大学毕业，硕士，助教，主要从事神经与组织解剖学方面的研究。 shy80825@yahoo.com.cn
  • 基金资助:

    新乡医学院高层次人才科研基金(08SSKYQD-001)。

Expression of transforming growth factor beta 1 in regenerated liver tissues following partial hepatectomy in rats

Song Hai-yan1, Gao Zhi-tao2, Liu Heng-xing1   

  1. 1 Department of Human Anatomy, 2 Research Center of Immunology, Xinxiang Medical University, Xinxiang  453003, Henan Province, China
  • Online:2010-10-29 Published:2010-10-29
  • Contact: Liu Heng-xing, Master, Professor, Department of Human Anatomy, Xinxiang Medical University, Xinxiang 453003, Henan Province, China liuhengxing@hotmail.com
  • About author:Song Hai-yan★, Master, Assistant lecturer, Department of Human Anatomy, Xinxiang Medical University, Xinxiang 453003, Henan Province, China shy80825@yahoo.com.cn
  • Supported by:

    the Scientific Research for High-level Talents in Xinxiang Medical University, No. 08SSKYQD-001*

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摘要：

背景：肝部分切除的安全性高低依赖于余肝的再生能力，了解肝硬化情况下肝部分切除后的肝再生情况，对肝硬化患者的治疗及预后具有重要意义。
目的：探讨转化生长因子β1在肝硬化大鼠肝部分切除后肝组织中的表达和意义。
方法：正常组大鼠不施加任何干预因素，模型组大鼠建立肝硬化模型，实验组大鼠建立肝硬化模型后行肝部分切除，复制肝再生模型。运用免疫组织化学方法和Western blotting技术，检测各组肝部分切除后12，24，72 h肝组织中转化生长因子β1的表达。
结果与结论：与模型组比较，实验组术后12，24 h肝组织转化生长因子β1的表达无明显差异(P > 0.05)，术后72 h肝组织中转化生长因子β1的表达显著增强(P < 0.01)。提示硬化肝部分切除后早期转化生长因子β1表达无明显增加，主要从中晚期可能通过与其他细胞因子的相互作用，继而开始发挥调节肝再生的功能。

关键词: 肝硬化, 肝再生, 肝切除, 部分, 转化生长因子&beta, 1, 细胞因子

Abstract:

BACKGROUND: The safety of partial hepatectomy depends on the regeneration capacity of residual livers. Thus, it is significant to understand the liver regeneration following partial hepatectomy for patients with cirrhosis.
OBJECTIVE: To investigate the expression and significance of transforming growth factor-β1 (TGF-β1) in liver tissue of hepatic cirrhosis after partial hepatectomy in rats.
METHODS: The model of hepatic cirrhosis was made in control group and model group, and then partial hepatectomy was deployed as regeneration models. The expressions of TGF-β1 in liver tissues were determined by immunohistochemical methods and Western blotting 12, 24, and 72 hours after partial hepatectomy.
RESULTS AND CONCLUSION: There was no significant difference between the model and regeneration groups in the expression of TGF-β1 in liver tissues at 12 and 24 hours after partial hepatectomy (P > 0.05), but the expression of TGF-β1 was notably increased at 72 hours (P < 0.01). The findings demonstrated that the expression of TGF-β1 in liver tissue has no strong increase at the early stage after partial hepatectomy, which can promote liver regeneration mainly from the later stage interacted with other factors.

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