中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (40): 7551-7554.doi: 10.3969/j.issn.1673-8225.2010.40.032

• 干细胞学术探讨 stem cell academic discussion • 上一篇    下一篇

间充质干细胞培养与分化诱导的特点及机制

赵  林1，申延清1，荣  春1，周岩冰2，夏长所3   

  1. 青岛大学医学院附属医院，1手术室，2普外科，  3骨科，山东省青岛市  266003
  • 出版日期:2010-10-01 发布日期:2010-10-01
  • 通讯作者: 周岩冰，硕士，教授，青岛大学医学院附属医院普外科，山东省青岛市 266003 zouybin@163.com 夏长所，博士，副教授，青岛大学医学院附属医院骨科，山东省青岛市 266003 xcs009@163.com
  • 作者简介:赵林★，女，1970年生，山东省青岛市人，汉族，青岛大学医学院在读硕士，副主任护师，主要从事普通外科基础与护理方面的研究。yzhsyq666@163.com
  • 基金资助:

    青岛大学医学院附属医院博士科研基金(博2006-21)，课题名称“干细胞的基础与临床应用研究”。

Characteristics and mechanisms of mesenchymal stem cell culture and induced differentiation　

Zhao Lin, Shen Yan-qing, Rong Chun, Zhou Yan-bing, Xia Chang-suo   

  1. 1 Operation Room, 2 Department of General Surgery, 3 Department of Orthopaedics, Hospital Affiliated to Medical College, Qingdao University, Qingdao  266003, Shandong Province, China
  • Online:2010-10-01 Published:2010-10-01
  • Contact: Zhou Yan-bing, Master, Professor, Department of General Srugery, Hospital Affiliated to Medical College, Qingdao University, Qingdao 266003, Shandong Province, China 266003 xcs009@163.com Xia Chang-suo, Doctor, Associate professor, Department of Orthopaedics, Hospital Affiliated to Medical College, Qingdao University, Qingdao 266003, Shandong Province, China Xcs009@163.con
  • About author:Zhao Lin★, Studying for master’s degree, Associate chief nurse, Operation Room, Hospital Affiliated to Medical College, Qingdao University, Qingdao 266003, Shandong Province, China yzhsyq666@163.com
  • Supported by:

    the Doctor Scientific Research Fund of Hospital Affiliated to Medical College of Qingdao University, No. 2006-21*　

PDF

373

被引次数

20

摘要：

背景：间充质干细胞是目前备受关注的一类具有多向分化潜能的成体干细胞。
目的：对间充质干细胞分离、鉴别、生物学特性及应用前景做一综述。
方法：应用计算机检索Medline数据库(1996-01/2010-03)，以“mesenchymal stem cell” 为检索词；同时检索CNKI数据库(1979/2010-02)，以“间充质干细胞、培养、分化”为检索词。
结果与结论：共收集400篇关于间充质干细胞分离、培养、鉴定和分化的文献，中文86篇，英文314篇。排除发表时间较早、重复及类似研究，纳入36篇符合标准的文献。发现间充质干细胞分离、培养、鉴定虽然有多种方法，但还没有统一的标准，细胞分化诱导的机制仍不清楚，应用也存在诸多问题。

关键词: 间充质干细胞, 培养, 分化, 诱导, 综述文献　

Abstract:

BACKGROUND: Mesenchymal stem cells (MSCs) are a class of adult stem cells with multi-directional differentiation potential.
OBJECTIVE: To review MSCs separation, identification, characterization and application prospects.
METHODS: The Medline database was searched for articles published from January 1996 to March 2010. The key words were “mesenchymal stem cells”. The China National Knowledge Infrastructure database was retrieved for articles published from 1979 to February 2010. The key words were “mesenchymal stem cells, culture, differentiation”.
RESULTS AND CONCLUSION: A total of 400 articles concerning MSCs isolation, culture, identification and differentiation were collected, including 86 Chinese articles and 314 English articles. Published earlier, repeated articles and those with similar studies were excluded, and 36 articles in accordance with inclusion criteria were included. We found that there are many methods concerning MSCs isolation, culture and identification, but there is no uniform standard, the mechanism of inducing cell differentiation remains unclear, and there are many problems in application.　

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