中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (28): 5167-5171.doi: 10.3969/j.issn.1673-8225.2010.28.009

• 皮肤粘膜组织构建 skin and mucosal tissue construction • 上一篇    下一篇

外加直流电场对内皮细胞增殖与迁徙的影响

黄文丽，赵颖异，杨  刚，赵晓军   

  1. 四川大学纳米生物医学技术与膜生物学研究所，四川省成都市 610041
  • 出版日期:2010-07-09 发布日期:2010-07-09
  • 通讯作者: 赵晓军，美国加州大学博士，教授，四川大学纳米生物医学技术与膜生物学研究所，四川省成都市 610041
  • 作者简介:黄文丽☆，女，1983年生，四川省盐源县人，汉族，四川大学在读博士，主要从事细胞与分子生物学研究。 wenlih_1111@yahoo.cn
  • 基金资助:

    四川大学“985”项目资助。

Effects of external direct current electric field on endothelial cell proliferation and migration  

Huang Wen-li, Zhao Ying-yi, Yang Gang, Zhao Xiao-jun   

  1. Institute for Nanobiomedical Technology and Membrane Biology, Sichuan University, Chengdu  610041, Sichuan Province, China
  • Online:2010-07-09 Published:2010-07-09
  • Contact: Zhao Xiao-jun, Doctor, Professor, Institute for Nanobiomedical Technology and Membrane Biology, Sichuan University, Chengdu 610041, Sichuan Province, China
  • About author:Huang Wen-li☆, Studying for doctorate, Institute for Nanobiomedical Technology and Membrane Biology, Sichuan University, Chengdu 610041, Sichuan Province, China wenlih_1111@yahoo.cn
  • Supported by:

    the “985” Project in Sichuan University*

PDF

352

被引次数

6

摘要：

背景：已有研究证明，电刺激可以引起内皮细胞的增殖、趋阴迁徙，同时伴随细胞分泌物释放的增加，从而调节血管张力和血管平滑肌细胞的生长，介导血管收缩和舒张。
目的：观察外加直流电场对人脐静脉内皮细胞HY926增殖和迁徙的影响。
方法：MTT 法检测在外加电压3，5， 8，10 V作用12 h后人脐静脉内皮HY926细胞的增殖情况。并选择内皮细胞增殖最佳的外加电压5 V，在5 V外加电压作用10 h内追踪人脐静脉内皮HY926细胞的运动轨迹，包括细胞的平均累计移行距离和平均移行速率，细胞的平均最终位移大小及其方向，以及所有单个细胞每小时的平均追踪sine值。
结果与结论：在外加电压3，5，8，10 V下作用12 h，人脐静脉内皮细胞生长均受到不同程度的抑制，但在外加电压5 V，电场强度163.46 mV/cm时内皮细胞增殖情况最好。在该生理电场下培养72 h，发现细胞生长贴壁生长、增殖正常，初步说明该生物反应器的细胞相容性良好，并且随时间的延长，内皮细胞的平均移行速率呈下降趋势。在5 V外加电压作用 10 h后，内皮细胞整体明显持续朝向阴极方向迁徙。提示外加直流电场对人脐静脉内皮细胞增殖有显著的抑制作用，并且可诱导细胞定向向阴极迁徙。

关键词: 直流电场, 内皮细胞, 细胞增殖, 定向迁徙, 生物反应器, 人脐静脉内皮细胞

Abstract:

BACKGROUND: Previous studies showed that electric fields affect cell shape, cell motility and morphology, including endothelial cell proliferation and directional migration. This effect plays an important role in the angiogenesis and vascular injury repair system.
OBJECTIVE: To explore the effect of external direct current (DC) fields on the proliferation and migration of human umbilical vein endothelial cells HY926.
METHODS: MTT assay was performed to detect in the proliferation of human umbilical vein endothelial cells HY926 under the voltage of 3 V, 5 V, 8 V, 10 V roles for 12 hours. The movement trajectory of the human umbilical vein endothelial cells under the voltage of 5 V for 10 hours were tracked and analyzed statistically, including the average cumulative distance and the average rate of cell migration, the average cell size and direction of the ultimate displacement (indicated using the sine value), and the average sine value of all single cell in an hour.
RESULTS AND CONCLUSION: After treatment with the voltage of 3 V, 5 V, 8 V, 10 V for 12 hours, the cells growth were inhibited remarkably. However, having been applied voltage of 5 V, the electric field strength of 163.46 mV / cm, the proliferation of endothelial cells performed the best. In the physical electric field, the average migration rate of endothelial cells showed a downward trend with the time increasing. After applied voltage of 5 V for 10 hours, all most of the endothelial cells obviously migrated towards the cathode continuously. It suggested that the applied DC electric field significantly inhibited the proliferation of the human umbilical vein endothelial cells, and induced cell migration directed to the cathode.

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