中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (28): 5163-5166.doi: 10.3969/j.issn.1673-8225.2010.28.008

• 皮肤粘膜组织构建 skin and mucosal tissue construction • 上一篇    下一篇

人角膜基质成纤维细胞分泌作用与脂多糖的影响

姜  宏1，许海洋2，郝继龙1    

  1. 1吉林大学中日联谊医院眼科，吉林省长春市 130031；2吉林大学第一医院神经外科，吉林省长春市  130011
  • 出版日期:2010-07-09 发布日期:2010-07-09
  • 通讯作者: 郝继龙，博士，教授，主任医师，吉林大学中日联谊医院眼科，吉林省长春市 130031
  • 作者简介:姜 宏☆，女，1976年生，吉林省舒兰市人，汉族，2000年白求恩医科大学毕业，博士，主治医师，主要从事角膜病研究。 jljh@7606@163.com
  • 基金资助:

    国家自然科学基金资助项目(30772384)，课题名称：Triptolide 抑制角膜基质胶原降解及角膜溃疡的机制；吉林省科技发展计划资助项目(20090173，20090744)，课题名称：TPCA-1对脂多糖诱导的角膜基质细胞分泌的抑制作用(20090173)，细胞信号转导机制在角膜溃疡发生的作用(20090744)。

Effect of lipopolysaccharide on the secretion of human corneal stromal fibroblasts

Jiang Hong1, Xu Hai-yang2, Hao Ji-long1   

  1. 1 Department of Ophthalmology, China-Japan Union Hospital of Jilin University, Changchun  130031, Jilin Province, China; 2 Department of Neurosurgery, First Hospital of Jilin University, Changchun  130011, Jilin Province, China
  • Online:2010-07-09 Published:2010-07-09
  • Contact: Hao Ji-long, Doctor, Professor, Chief physician, Department of Ophthalmology, China-Japan Union Hospital of Jilin University, Changchun 130031, Jilin Province, China
  • About author:Jiang Hong☆, Doctor, Attending physician, Department of Ophthalmology, China-Japan Union Hospital of Jilin University, Changchun 130031, Jilin Province, China jljh@7606@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30772384*; the Technology Research and Development Program of Jilin Province, No. 20090173, 20090744**

PDF

360

被引次数

3

摘要：

背景：体外培养的角膜基质成纤维细胞，可以识别革兰阴性细菌细胞壁成分脂多糖，并释放多种细胞因子和趋化因子，从而促进角膜溃疡的形成。
目的：观察细菌脂多糖对角膜基质成纤维细胞分泌作用的影响。
方法：体外培养人角膜基质细胞，取第3~5代细胞鉴定后用于实验。采用ELISA法检测角膜基质细胞脂多糖作用前后培养上清中分泌白细胞介素1，肿瘤坏死因子α，白细胞介素6，白细胞介素8水平。
结果与结论：脂多糖在一定质量浓度范围及作用时间内，对人角膜基质细胞增殖存活率没有影响。培养的人角膜基质成纤维细胞不表达白细胞介素1，肿瘤坏死因子α，未给予脂多糖的角膜基质细胞微量表达白细胞介素6，白细胞介素8，脂多糖(100 μg/L)作用24 h后，白细胞介素6，白细胞介素8水平明显增高。

关键词: 角膜基质细胞, 脂多糖, 成纤维细胞, 细胞培养, 白细胞介素

Abstract:

BACKGROUND: Corneal stromal fibroblasts cultured in vitro can recognize lipopolysaccharide, the main constituent of gram-negative bacterial cell wall, and release varied cytokines and chemotactic factors, which facilitate leukocytic infiltration and promote formation of corneal ulcer.
OBJECTIVE: To observe the effect of bacterial lipopolysacchride on the secretion of human corneal stromal fibroblasts.
METHODS: Human corneal stromal cells were cultured in vitro, and the 3-5 generations were selected for the experiment. The levels of interleukin 1, tumor necrosis factor α, interleukin 6 and interleukin 8 in supernatant of corneal stromal fibroblasts were determined by ELISA prior to and after adding lipopolysaccharide.
RESULTS AND CONCLUSION: In a certain range of mass concentration and action times, lipopolysaccharide had no effect on survival rate of human corneal stromal fibroblasts. No interleukin 1 and tumor necrosis factor α were expressed in the cultured human corneal stromal fibroblasts. However, interleukin 6 and interleukin 8 were slightly expressed in human corneal stromal fibroblasts without lipopolysaccharide. At 24 hours after adding lipopolysaccharide (100 μg/L), the levels of interleukin 6 and interleukin 8 were increased obviously.

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