中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (27): 4960-4963.doi: 10.3969/j.issn.1673-8225.2010.27.005

• 干细胞培养与分化 stem cell culture and differentiation • 上一篇    下一篇

骨髓间充质干细胞对系统性红斑狼疮患者T细胞活化的影响

董 毅1，刘 蕾2，夏瑞祥1，曾庆曙1，李庆生1   

  1. 蚌埠医学院第三附属医院，1血液内科，2风湿科，安徽省宿州市 234011
  • 出版日期:2010-07-02 发布日期:2010-07-02
  • 通讯作者: 李庆生，博士，教授，主任医师，安徽医科大学第一附属医院血液内科，安徽省合肥市 230022
  • 作者简介:董毅，男，1971年生，安徽省合肥市人，汉族，1994年安徽医科大学毕业，硕士，副主任医师，主要从事血液免疫学及干细胞研究。 dongyixx@126. com
  • 基金资助:

    安徽省教育厅自然科学基金资助项目(2006kj417B)

Effect of human bone marrow mesenchymal stem cells on T cell activation in patients with systemic lupus erythematosus

Dong Yi1, Liu Lei2, Xia Rui-xiang1, Zeng Qing-shu1, Li Qing-sheng1   

  1. 1Department of Hematology, 2Department of Rheumatology, the Third Affiliated Hospital of Bengbu Medical College, Suzhou  234011, Anhui Province, China
  • Online:2010-07-02 Published:2010-07-02
  • Contact: Li Qing-sheng, Doctor, Professor, Chief physician, Department of Hematology, the First Affiliated Hospital of Anhui Medical University, Hefei 230022, Anhui Province, China
  • About author:Dong Yi, Master, Associate chief physician, Department of Hematology, the Third Affiliated Hospital of Bengbu Medical College, Suzhou 234011, Anhui Province, China dongyixx@126.com
  • Supported by:

    A Grant from the Education Department of Anhui Province, No. 2006kj417B*

PDF

464

被引次数

21

摘要：

背景：骨髓间充质干细胞具有免疫调节作用，可治疗自身免疫性疾病。

目的：探讨在体外人骨髓间充质干细胞对系统性红斑狼疮患者T细胞活化的影响。

方法：分离、培养人骨髓间充质干细胞，培养至第3代以后的细胞胰蛋白酶消化后用流式细胞仪确定其浓度，按照不同浓度将骨髓间充质干细胞设为1×108，1×107L-1两组。取肝素抗凝新鲜27例系统性红斑狼疮患者患者外周血10 mL，分离培养T淋巴细胞，以1×109 L-1的细胞浓度，100 µL/孔，接种两组骨髓间充质干细胞上，以单纯统性红斑狼疮患者的T淋巴细胞作为对照组。通过流式细胞术计算CD3+ T细胞CD25(IL-2R)和CD38细胞的表达率。

结果与结论：与对照组比较，1×108 L-1浓度组的骨髓间充质干细胞对系统性红斑狼疮患者CD25及CD38的表达呈明显抑制作用(P < 0.01)，1×107 L-1浓度组，无明显变化。结果表明：骨髓间充质干细胞抑制系统性红斑狼疮患者T细胞的活化，且对这种抑制作用具有数量依赖性。

关键词: 系统性红斑狼疮, 骨髓间充质干细胞, T淋巴细胞, 自身免疫性疾病, 流式细胞仪

Abstract:

BACKGROUND: Bone marrow mesenchymal stem cells (BMMSCs) have immunological effects and can treat autoimmune disease.

OBJECTIVE: To explore the effect of human BMMSCs on T cell activation in patients with systemic lupus erythematosus (SLE) in vitro.

METHODS: BMMSCs were cultured and the 3rd passage cells were digested by trypsin. The concentration of cells was identified by flow cytometry. And MMSCs were divided into 1×108/L and 1×107/L group. Totally 10 mL peripheral blood lymphocytes (PBL) were obtained from 27 cases with SLE and incubated into BMMSCs with concentration of 1×109/L, 100 µL per well. Expression rates of CD3+ T cells CD25 (IL-2R) and CD38 were calculated by flow cytometry.

RESULTS AND CONCLUSION: Compared with the control group, the expression of CD25 and CD38 were obviously suppressed in the 1×108/L group (P < 0.01). But there was no significant change in the 1×107/L group. The results demonstrated that BMMSCs inhibit T cell activation in patients with SLE in a dose-dependent manner.

中图分类号: