中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (23): 4281-4284.doi: 10.3969/j.issn.1673-8225.2010.23.023

• 干细胞移植 stem cell transplantation • 上一篇    下一篇

造血干细胞动员和采集对正常供者的影响

陈晓霞，王智明，罗贤生，徐丹丹，李  兴，雷美清   

  1. 中南大学湘雅医学院附属海口市人民医院血液科，海南省海口市 570208
  • 出版日期:2010-06-04 发布日期:2010-06-04
  • 作者简介:陈晓霞，女，1962年生，江西省武宁县人，汉族，1985年江西医学院毕业，副主任医师，主要从事血液病及造血干细胞移植方面的研究。 chenxia_62@ yahoo.com.cn
  • 基金资助:

    2005年海南省自然科学基金项目(80571)

Effects of mobilization and collection of hemopoietic stem cells on normal donors

Chen Xiao-xia, Wang Zhi-ming, Luo Xian-sheng, Xu Dan-dan, Li Xing, Lei Mei-qing   

  1. Department of Hematology, Haikou People’s Hospital Affiliated to Xiangya School of Medicine, Central South University, Haikou 570208, Hainan Province, China
  • Online:2010-06-04 Published:2010-06-04
  • About author:Chen Xiao-xia, Associate chief physician, Department of Hematology, Haikou People’s Hospital Affiliated to Xiangya School of Medicine, Central South University, Haikou 570208, Hainan Province, China chenxia_62@yahoo. com.cn
  • Supported by:

    the Natural Science Foundation of Hainan Province in 2005, No. 80571*

PDF

497

被引次数

5

摘要：

背景：粒细胞集落刺激因子动员是当前健康志愿供者采用的主要方法。据国内外文献报告：在粒细胞集落刺激因子动员中已发生少数严重不良反应，这引起了国人对中国非血缘健康供者安全的忧虑。粒细胞集落刺激因子作为动员剂对健康志愿供者有何影响？是否安全？

目的：观察粒细胞集落刺激因子对正常供者的影响。

方法：选择2003-01/2008-12在海口市人民医院接受粒细胞集落刺激因子5~10 μg/(kg·d)动员剂进行外周血造血干细胞捐赠者16例，观察动员及采集过程的不良反应，检测动员前后血常规CD3、CD19、CD3+4、CD3+8细胞比值在动员前后变化，并随访所有供者。

结果与结论：10例供者在重组人粒细胞集落刺激因子动员过程中无任何不适，有3例出现低热，头痛、肌肉及骨骼疼痛、腰痛等，3例供者出现发热，其严重程度均在Ⅰ级，但无需终止动员。白细胞经重组人粒细胞集落刺激因子动员后数量较动员前升高，停止动员后3 d全部供者的白细胞恢复至动员前水平。血红蛋白、血小板、CD3、CD19、CD3+4、CD3+8细胞比值在动员前、动员后72，96 h无明显变化。提示健康供者可耐受粒细胞集落刺激因子5~10 μg/(kg·d)，动员和采集过程；且对T细胞亚群无影响。

关键词: 粒细胞集落刺激因子, 造血干细胞, 供者, 动员, 安全性

Abstract:

BACKGROUND: Mobilization of granulocyte colony-stimulating factor (G-CSF) is a main method used in healthy voluntary donor. Previous studies have demonstrated that some severe adverse effects occurred in mobilization of G-CSF. This leads to anxiety of the safety of unrelated healthy donors. Will the mobilization of granulocyte colony-stimulating factor have any effect on healthy voluntary donor? It remains unknown whether it is safe?

OBJECTIVE: To investigate the effect of G-CSF on healthy donors.

METHODS: A total of 16 healthy donors were mobilized using G-CSF at a dose of 5-10 μg/kg per day at Haikou People’s Hospital from January 2003 to December 2008. The adverse events were recorded during the process of mobilization and collection. Percentage of CD3, CD19, CD3+4, CD3+8 cells in blood was detected prior to and following mobilization. All donors were followed-up.

RESULTS AND CONCLUSION: No adverse effects were determined during G-CSF mobilization in 10 donors. Three cases affected low-grade fever, headache, muscle and skeleton pain or lumbago. Three cases suffered from fever. Severity was in grade I, which does not cause the termination of the mobilization. Leukocyte number was greater following G-CSF mobilization than prior to mobilization. Leukocyte count returned to pre-mobilization levels at 3 days following stopping mobilization. There was no significant difference in hemoglobin and platelet counts, the percentage of CD3, CD19, CD3+4, CD3+8 cells in blood between pre-mobilization and 72 and 96 hours post-mobilization. Results have indicated that the healthy donors can tolerate the mobilization at a dose of 5-10 μg/kg per day, and G-CSF dose not affect the lymphocyte subsets.

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