中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (23): 4211-4216.doi: 10.3969/j.issn.1673-8225.2010.23.008

• 干细胞培养与分化 stem cell culture and differentiation • 上一篇    下一篇

大鼠脂肪和骨髓来源间充质干细胞的生物学特性比较

杨中萌1，尹震宇1，叶志坚1，张 琼2，赵文秀1，王效民1   

  1. 1厦门大学附属中山医院肝胆外科，福建省厦门市361004；
    2厦门大学生命科学学院，福建省厦门市361005
  • 出版日期:2010-06-04 发布日期:2010-06-04
  • 通讯作者: 王效民，教授，博士生导师，厦门大学附属中山医院肝胆外科，福建省厦门市 361004
  • 作者简介:杨中萌，男， 1982年生，山东省济宁市人，汉族，2010年厦门大学毕业，硕士，主要从事器官移植免疫耐受研究。 joyyoung525@ 126.com
  • 基金资助:

    卫生部科学研究基金——福建省卫生教育联合攻关计划资助（WKJ2005-2-020）
    厦门市科技计划社会发展项目（3502Z20064020）

Biological characteristics of mesenchymal stem cells from bone marrow versus adipose tissue in rats

Yang Zhong-meng1, Yin Zhen-yu1, Ye Zhi-jian1, Zhang Qiong2, Zhao Wen-xiu1, Wang Xiao-min1   

  1. 1Department of Hepatobiliary Surgery, Zhongshan Hospital, Xiamen University, Xiamen  361004, Fujian Province, China;
    2School of Life Sciences, Xiamen University, Xiamen  361005, Fujian Province, China
  • Online:2010-06-04 Published:2010-06-04
  • Contact: Wang Xiao-min, Professor, Doctoral supervisor, Department of Hepatobiliary Surgery, Zhongshan Hospital, Xiamen University, Xiamen 361004, Fujian Province, China
  • About author:Yang Zhong-meng, Master, Department of Hepatobiliary Surgery, Zhongshan Hospital, Xiamen University, Xiamen 361004, Fujian Province, China joyyoung525@126. com
  • Supported by:

    the Science Research Foundation of Ministry of Health ＆ the Key Program of Fujian Provincial Health and Education United Project, No. WKJ2005-2-020*;
    the Social Development Program of Science and Technology Project of Xiamen City, No. 3502Z20064020*

PDF

421

被引次数

8

摘要：

背景：除骨髓外，人们已从胎盘组织、脐带血肌肉组织、脂肪组织中分离到了间充质干细胞。
目的：比较大鼠脂肪和骨髓间充质干细胞生物学特性和免疫调节功能的差异。
方法：脂肪间充质干细胞和骨髓间充质干细胞分别来自BN大鼠的脂肪和骨髓。体外分离、纯化脂肪和骨髓来源间充质干细胞，进行细胞形态、表面标志、生长动力学、分化潜能鉴定；混合淋巴细胞反应比较两种细胞的免疫调节特性。
结果与结论：脂肪间充质干细胞与骨髓间充质干细胞光镜和透射电镜下形态相似，第3代的脂肪间充质干细胞和骨髓间充质干细胞均高表达CD29，CD90，低表达CD34，CD45，CD11b；第3，4，5代脂肪间充质干细胞增殖速度明显快于骨髓间充质干细胞；两者都具有低免疫原性，可以抑制异基因抗原引起的T淋巴细胞增殖，且这种抑制作用与细胞数目成正相关，等量脂肪间充质干细胞和骨髓间充质干细胞抑制作用差异无显著性意义。结果证实脂肪间充质干细胞具有和骨髓间充质干细胞同样的低免疫原性和免疫调节功能。

关键词: 脂肪间充质干细胞, 脂肪组织, 骨髓间充质干细胞, 免疫调节, 大鼠

Abstract:

BACKGROUND: Except bone marrow, people have collected mesenchymal stem cells (MSCs) from placenta tissue, cord blood muscle tissue and adipose tissue.
OBJECTIVE: To compare the difference in biological characteristics and immunoloregulation of rat adipose-derived MSCs (ADSCs) and bone marrow MSCs (BMSCs).
METHODS: ADSCs and BMSCs were obtained respectively from adipose and bone marrow of BN rats. ADSCs and BMSCs were isolated and purified in vitro to determine cell morphology, surface marker, growth kinetics and differentiation potential. Immunoloregulation of ADSCs and BMSCs was compared in mixed lymphocyte response
RESULTS AND CONCLUSION: Morphology was similar between ADSCs and BMSCs under an optical microscope and transmission electron microscope. ADSCs and BMSCs at passage 3 highly expressed CD29 and CD90, weakly expressed CD34, CD45 and CD11b. The proliferation speed of ADSCs at passages 3, 4 and 5 was obviously faster than BMSCs. Both ADSCs and BMSCs possessed low immunogenicity, could suppress allogenic antigen-induced T lymphocyte proliferation. Moreover, this inhibitory effect was positively associated with cell number. There were no significant differences in inhibitory effects of an equal volume of ADSCs and BMSCs. Results verified that ADSCs had the same low immunogenicity and immunoloregulation as BMSCs.

中图分类号: