中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (11): 2004-2007.doi: 10.3969/j.issn.1673-8225.2010.11.026

• 组织构建基础实验 basic experiments in tissue construction • 上一篇    下一篇

复方中药对小鼠运动能力及其生化指标影响的比较

王艳琼1，屈红林2，杨永亮1   

  1. 1广西师范大学体育学院，广西壮族自治区桂林市 541004；
    2江西省宜春学院体育学院，江西省宜春市  336000
  • 出版日期:2010-03-12 发布日期:2010-03-12
  • 作者简介:王艳琼，女，1974年生，汉族，2008年广西师范大学毕业，硕士，讲师，主要从事运动人体科学研究。quhonglin20040125@126.com

Influence of different Chinese medical compounds on mouse exercise capacity and biochemical indexes

Wang Yan-qiong1, Qu Hong-lin2, Yang Yong-liang1   

  1. 1College of Physical Education, Guangxi Normal University, Guilin   541004, Guangxi Zhuang Autonomous Region, China;
    2College of Physical Education, Yichun University, Yichun   336000, Jiangxi Province, China
  • Online:2010-03-12 Published:2010-03-12
  • About author:Wang Yan-qiong, Master, Lecturer, College of Physical Education, Guangxi Normal University, Guilin 541004, Guangxi Zhuang Autonomous Region, China quhonglin20040125@126.com

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373

被引次数

2

摘要：

背景：有关中药对动物运动能力的实验研究较多，能够真正体现出复方中药对提高运动员机体运动能力的研究并不多，尤其缺乏对复方中药进行对比的研究。
目的：观察不同复方中药制剂对小鼠运动能力的影响，筛选作用明确的复方制剂。
方法：8周龄纯系雄性昆明种小鼠80只随机数字表法分成8组，每组10只：对照组、复方一~七组，各组动物体质量差异无显著性意义。复方一的主要成分是血竭、麝香，复方二的主要成分为秦归、白术、白芍，复方三的主要成分为白术、白芷、三七，复方四的主要成分为白芷、乳香、当归和甘草，复方五的主要成分为归尾，复方六的主要成分为川红花、丹参，复方七的主要成为当归、麝香等，其中复方一~五为验方，复方六为训练队常用经验药物，复方七为中医配置药方。复方一~七组灌喂相应中药复方1.17，0.78，0.78，1.17，7.02，5.53，11.70 g/(kg•d)，4周。对照组灌胃给予生理盐水。灌胃给药第4~6天进行20 min/d无负重的适应性游泳训练3 d，休息1 d后每周进行6 d的游泳训练，强度由30 min/d逐渐递增，递增率为10 min/d，持续2 h，然后进行负重游泳，由小鼠自身体质量2%进行负重游泳，每天以1%的递增率逐渐增加负荷直到实验结束。在整个实验过程中，每周安排一次大强度的游泳训练，使各小鼠达到力竭状态。观察小鼠训练情况以及中药干预4周后血清肌酸激酶和乳酸脱氢酶的变化。
结果与结论：80只小鼠均进入结果分析。各复方药物对小鼠体质量影响不明显，复方二和复方六药物组小鼠的力竭时间最长。各中药复方对小鼠运动后血清肌酸激酶活性均有一定的抑制作用，但以复方六和复方二效果较好(P < 0.05)。与对照组比较，各复方组小鼠血清乳酸脱氢酶活性均下降，但仅与复方二、六组差异有显著性意义(P < 0.05)。提示复方药物二和复方药物六能显著提高小鼠的运动能力，延迟运动机体骨骼肌维系的损伤，其机制可能与复方药物中所含的主要成分当归、川红花、丹参等有密切关系。

关键词: 复方中药, 运动能力, 肌酸激酶, 乳酸脱氢酶, 组织工程

Abstract:

BACKGROUND: Many studies concerning influence of traditional Chinese medicine on animal movement capacity have been conducted. However, studies on the improvement of exercise capacity by traditional Chinese medicine are few, especially comparisons among traditional Chinese medicine
OBJECTIVE: To explore the influence of different traditional Chinese medicine preparations on the exercise capacity in mice to screen compound preparations with precise effects.
METHOD: A total of 80 8-week-old pure male Kunming mice were randomly assigned to 8 groups (n=10): control and compound 1-7 groups. There were no significant differences in body mass of all groups. The compound 1 comprised Xuejie and Shexiang; compound 2 comprised Qingui, Baizhu and Baishao; compound 3 comprised Baizhu, Baizhi and Sanqi; compound 4 comprised Baizhi, Ruxiang, Danggui and Radix glycryrrhizae; compound 5 comprised Guiwei; compound 6 comprised Sichuan Honghua and Danshen; compound 7 comprised Danggui and Shexiang. Of the 7 prescriptions, compound 1-5 were empirical formula, compound 6 was common medicine used during training, and compound 7 was prescription of traditional Chinese medicine. The 7 compound groups were respectively treated with 1.17, 0.78, 0.78, 1.17, 7.02, 5.53, and 11.70 g/kg per day for 4 weeks, and control group was intragastrically infused with normal saline. From day 4 to 6, load-free swimming training was performed 20 minutes a day for 3 days, followed by swimming for 6 days a week after 1 day of rest. The intensity was gradually increased from 30 minutes per day by 10 minutes per day for 2 hours, followed by loading swimming with load of 2% of the body mass, increased by 1% per day until the final experiment. During the entire procedure, a great intensity swimming training was performed every week to make the mice exhausted. Mice training and serum creatine kinase and lactate dehydrogenase 4 weeks following treatment were observed.
RESULTS AND CONCLUSION: A total of 80 mice were included in the final analysis. The compounds showed little influence on mice body mass; the compound 2 and compound 6 groups had longest exhaustive time. All compounds inhibited serum creatine kinase activity following exercise, in particular compound 6 and 2 (P < 0.05). Compared with control group, the serum lactate dehydrogenase activity of each compound group was decreased, in particular, compound 2 and 6 groups showed significant differences (P < 0.05). Compound 2 and 6 significantly enhanced the exercise capacity of mice and delayed injury of body. The mechanism may highly relate to components in the compounds, Danggui, Sichuan Honghua and Danshen.

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