中国组织工程研究

中国组织工程研究 ›› 2026, Vol. 30 ›› Issue (23): 5934-5942.doi: 10.12307/2026.311

• 组织构建综述 tissue construction review • 上一篇    下一篇

中药复方治疗腰椎间盘退变动物实验：物种选择、造模方法及给药方式

谢子颖1，黎松波1，2，黎建文2，尹煜超2，郑百川1，胡承尚2   

  1. 1广东医科大学第一临床医学院，广东省湛江市   524023；2南方医科大学第十附属医院(东莞市人民医院)，东莞市骨科常见骨病基础、临床与数字化研究重点实验室，广东省东莞市   523069
  • 收稿日期:2025-04-12 接受日期:2025-06-07 出版日期:2026-08-18 发布日期:2025-12-30
  • 通讯作者: 黎松波，硕士，主任医师，教授，广东医科大学第一临床医学院，广东省湛江市 524023；南方医科大学第十附属医院(东莞市人民医院)，东莞市骨科常见骨病基础、临床与数字化研究重点实验室，广东省东莞市 523069
  • 作者简介:谢子颖，男，1998年生，广东省东莞市人，汉族，广东医科大学在读硕士，医师，主要从事骨科方面的研究。
  • 基金资助:
    广东省基础与应用基础研究基金项目(2021B1515140056)，课题名称：可穿透软骨终板的纳米载体靶向递送三七皂苷抑制椎间盘早期退变的作用及机制研究，项目负责人：黎松波；东莞市社会发展科技项目(20221800906282)，课题名称：镁/羟基磷灰石涂层3D打印多孔钛合金植入物用于脊柱椎板重建成骨和血管化的研究，项目负责人：黎建文

Animal experimental study on the treatment of lumbar intervertebral disc degeneration with Chinese herbal compound: species selection, modeling method and drug administration

Xie Ziying1, Li Songbo1, 2, Li Jianwen2, Yin Yuchao2, Zheng Baichuan1, Hu Chengshang2   

  1. 1The First Clinical School of Medicine, Guangdong Medical University, Zhanjiang 524023, Guangdong Province, China; 2The Tenth Affiliated Hospital of Southern Medical University (Dongguan People’s Hospital), Dongguan Key Laboratory of Basic, Clinical and Digital Research on Orthopedic Common Bone Diseases, Dongguan 523069, Guangdong Province, China
  • Received:2025-04-12 Accepted:2025-06-07 Online:2026-08-18 Published:2025-12-30
  • Contact: Li Songbo, MS, Chief physician, Professor, The First Clinical School of Medicine, Guangdong Medical University, Zhanjiang 524023, Guangdong Province, China; The Tenth Affiliated Hospital of Southern Medical University (Dongguan People’s Hospital), Dongguan Key Laboratory of Basic, Clinical and Digital Research on Orthopedic Common Bone Diseases, Dongguan 523069, Guangdong Province, China
  • About author:Xie Ziying, MS candidate, Physician, The First Clinical School of Medicine, Guangdong Medical University, Zhanjiang 524023, Guangdong Province, China
  • Supported by:
    Fundamental and Applied Basic Research Funds of Guangdong Province, No. 2021B1515140056 (to LSB); Dongguan Social Development Science and Technology Project, No. 20221800906282 (to LJW)

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摘要：


文题释义：
中药复方：由2种或2种以上的中药单味药材按照一定的比例和配伍规则组合而成的药物配方，这些单味药材经过合理搭配后能够协同作用、增强疗效，或发挥不同的药理作用。
腰椎间盘退变：常见的脊柱疾病，主要表现为腰椎间盘的结构和功能发生退行性改变。

背景：中药复方对腰椎间盘退变具有独特的疗效。为了深入了解其作用机制，研究者们开展了许多动物体内实验，然而在动物模型、给药方式和观察指标的选择上并未达成一致。
目的：综述了中药复方治疗腰椎间盘退变的动物实验的研究进展，重点综述腰椎间盘退变动物模型制备、中药复方给药方式及疗效评估指标，旨在为相关研究提供参考。
方法：应用计算机检索建库至2024年11月为止的中国知网(CNKI)、万方、维普、PubMed、Web of Science数据库，中文检索词为中药，复方，中医药，草药，药物组合，汤，方，腰痛，椎间盘退行性病，椎间盘退变，椎间盘损伤，椎间盘退化，椎间盘疾病，动物，鼠，兔，犬，猪，羊，猴，灵长类，模型，造模，实验，研究，进展，综述；英文检索词为traditional Chinese medicine，Chinese herbal medicine，Chinese medicine formula，herbal formula，intervertebral disc degeneration，degenerative disc disease，disc degeneration，intervertebral disc injury，animal model，animal experiment，preclinical study，rat，mouse，rabbit，sheep，dog，pig，non-human primate，monkey，primate，treatment，therapy，effect，intervention。共检索到 789 篇相关文献，139篇文献符合纳入标准。
结果与结论：①采用中药复方治疗椎间盘退变的研究者更青睐采用大鼠、沙鼠、豚鼠、小鼠或新西兰兔作为实验动物；②各类椎间盘退变造模方式各有优劣，纤维环穿刺模型是此类研究中应用最为广泛的模型，多种方式联合造模能更好地模拟椎间盘退变进程；③中药复方在动物模型上的给药方式与人体给药方式保持一致，以经口给药为主；④中药复方对椎间盘退变的治疗效果良好，常通过影像学、行为学、分子生物学、组织学验证。
https://orcid.org/0009-0009-5269-6242 (谢子颖) 


中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

关键词: 中药复方, 椎间盘退变, 腰椎间盘退行性疾病, 动物模型, 体内实验, 实验设计, 研究进展

Abstract: BACKGROUND: Chinese herbal compound has a unique curative effect on lumbar disc degeneration. In order to further understand the mechanism of its action, researchers have carried out many animal experiments in vivo, but there is no agreement on the animal model, the way of administration and the choice of observation indicators.
OBJECTIVE: To review the progress in animal experimental studies on the treatment of lumbar disc degeneration with Chinese herbal compounds, focusing on the preparation of animal models of lumbar disc degeneration, the administration methods of Chinese herbal compounds and the evaluation indexes of efficacy, in order to provide a reference for related studies.
METHODS: CNKI, WanFang, VIP, PubMed and Web of Science were retrieved by computer for relevant literature published from database inception to November 2024. The Chinese terms were “Chinese medicine, compound, Chinese medicine, herbal, drug combination, soup, formula, low back pain, degenerative disc disease, disc degeneration, disc injury, disc degeneration, disc disease, animal, rat, rabbit, canine, porcine, sheep, monkey, primate, model, modeling, experimental, research, progress, review.” The English search terms were “traditional Chinese medicine, Chinese herbal medicine, Chinese medicine formula, herbal formula, intervertebral disc degeneration, degenerative disc disease, disc degeneration, intervertebral disc injury, animal model, animal experiment, preclinical study, rat, mouse, rabbit, sheep, dog, pig, non-human primate, monkey, primate, treatment, therapy, effect, intervention.” A total of 789 relevant articles were retrieved, 139 of which met the inclusion criteria.
RESULTS AND CONCLUSION: (1) Rats, gerbils, guinea pigs, mice or New Zealand rabbits are preferred as experimental animals for the treatment of intervertebral disc degeneration by using Chinese herbal compounds. (2) All kinds of intervertebral disc degeneration modeling methods have their own advantages and disadvantages, and the annulus fibropuncture model is the most widely used model in this kind of research. Combination of multiple modeling methods can better simulate the progression of intervertebral disc degeneration. (3) The mode of administration of Chinese herbal compounds in animal models is consistent with that in humans, and oral administration is the main method. (4) Chinese herbal compounds have a good effect on the treatment of disc degeneration, which is often verified by imaging, behavioral, molecular biology and histology.


Key words: Chinese herbal compound, intervertebral disc degeneration, lumbar disc degenerative disease, animal model, in vivo experiment, experimental design, research progress

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