中国组织工程研究

中国组织工程研究 ›› 2023, Vol. 27 ›› Issue (2): 282-286.doi: 10.12307/2022.1003

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

川芎嗪改善脊髓完全横断大鼠血液流变学指标的动态观察

刘  港1，邓博文1，蒋昇源1，徐  林1，范  筱1，2，陶经纬1，张厚君1，贺  丰1，赵  毅1，穆晓红1   

  1. 1北京中医药大学东直门医院，北京市  100700；2青岛市市立医院，山东省青岛市  266000
  • 收稿日期:2021-11-18 接受日期:2021-12-20 出版日期:2023-01-18 发布日期:2022-06-20
  • 通讯作者: 穆晓红，博士，教授，主任医师，博士生导师，北京中医药大学东直门医院，北京市 100700
  • 作者简介:刘港，男，1997 年生，山东省菏泽市人，汉族，北京中医药大学在读硕士，主要从事脊髓损伤、脊柱退行性病变及脑性瘫痪的中西医结合治疗。
  • 基金资助:
    国家自然科学基金面上项目(81874467)，项目负责人：穆晓红

Tetramethylpyrazine improves hemorheological indexes in rats with complete spinal cord transection: a dynamic observation

Liu Gang1, Deng Bowen1, Jiang Shengyuan1, Xu Lin1, Fan Xiao1, 2, Tao Jingwei1, Zhang Houjun1, He Feng1, Zhao Yi1, Mu Xiaohong1   

  1. 1Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China; 2Qingdao Municipal Hospital, Qingdao 266000, Shandong Province, China
  • Received:2021-11-18 Accepted:2021-12-20 Online:2023-01-18 Published:2022-06-20
  • Contact: Mu Xiaohong, MD, Professor, Chief physician, Doctoral supervisor, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China
  • About author:Liu Gang, Master candidate, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China
  • Supported by:
    the National Natural Science Foundation of China, No. 81874467 (to MXH)

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摘要：

文题释义：
川芎嗪：为川芎的有效活性成分之一，在治疗脊髓损伤方面具有改善微循环障碍、抑制炎症反应、调控神经细胞凋亡等作用。
血液流变学：研究范围包括血液的流动性、黏滞性、凝固性以及细胞的变形性等病理生理变化，对于疾病的诊断、预防、治疗具有重要意义。

背景：脊髓损伤后血液流变学指标的改变会引起微循环障碍，诱导组织缺血缺氧，加剧脊髓组织继发性损伤。
目的：观察川芎嗪干预脊髓完全横断大鼠后不同时间节点血液流变学指标的变化。
方法：将54只8周龄雌性SD大鼠随机分为假手术组、模型组、川芎嗪组，每组18只。假手术组仅行椎板切除术，模型组、川芎嗪组采用自制双刃显微剪行T10脊髓完全横断，缺损间隙2 mm，川芎嗪组在模型制备后给予200 mg/(kg·d)盐酸川芎嗪注射液腹腔注射，连续5 d。分别于造模术后7，14，28 d进行腹主动脉取血，检测全血黏度(低、中、高切边率)、血浆黏度、红细胞压积、红细胞聚集指数、红细胞变形指数、红细胞刚性指数、红细胞电泳指数。
结果与结论：①脊髓损伤后第7，14，28天，模型组全血黏度(低、高、中切变率)和血浆黏度均显著高于假手术组(P < 0.05)；第14天，模型组红细胞压积显著高于假手术组(P < 0.05)，红细胞刚性指数、红细胞电泳指数显著低于假手术组(P < 0.05)；②脊髓损伤后第7天，川芎嗪能够显著降低全血黏度(低、高、中切变率)和血浆黏度(P < 0.05)；第14天川芎嗪能够升高红细胞变形指数(P < 0.05)，一定程度上也能改善血液黏度相关指标，但差异无显著性意义(P > 0.05)；第28天川芎嗪能够显著改善全血黏度(低切)和血浆黏度(P < 0.05)；③脊髓损伤后，全血黏度及血浆黏度均会升高，红细胞相关指数也会出现不同程度的变化，川芎嗪的使用能够改善早期血液流变学指标的恶化，对于改善微循环障碍具有一定的促进作用。

https://orcid.org/0000-0002-6014-7261(刘港)

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

关键词: 川芎嗪, 脊髓损伤, 血液流变学, 微循环障碍, 血管新生, 内皮细胞

Abstract: BACKGROUND: Hemorheological changes following spinal cord injury can cause microcirculation disorder, induce tissue ischemia and hypoxia, and aggravate the secondary injury to spinal cord tissue.
OBJECTIVE: To observe the changes in hemorheological indexes at different time points after tetramethylpyrazine intervention in rats with complete spinal cord transection.
METHODS: Fifty-four 8-week-old female Sprague-Dawley rats were randomly divided into three groups (n=18 per group): sham operation group, model group, and tetramethylpyrazine group. Only laminectomy was performed in the sham operation group, while the T10 segment was completely transected with a 2 mm defect gap in the model and tetramethylpyrazine groups by a self-made double-edge microshear. After model preparation, tetramethylpyrazine group was given intraperitoneal injection of tetramethylpyrazine hydrochloride, 200 mg/kg per day, for 5 consecutive days. Abdominal aorta blood samples were taken 7, 14, and 28 days after operation for measuring whole blood viscosity (low, high and medium shear rates), plasma viscosity, hematocrit, erythrocyte aggregation index, erythrocyte deformability index, erythrocyte rigidity index, and erythrocyte electrophoresis index.
RESULTS AND CONCLUSION: The whole blood viscosity (low, high, and medium shear rates) and plasma viscosity in the model group were significantly higher than those in the sham group at 7, 14, and 28 days after spinal cord injury (P < 0.05). Hematocrit was significantly higher but erythrocyte rigidity index and erythrocyte electrophoresis index were significantly lower in the model group than the sham group at 14 days after spinal cord injury (P < 0.05). Tetramethylpyrazine significantly reduced whole blood viscosity (low, high, and medium shear rates) and plasma viscosity at 7 days after spinal cord injury (P < 0.05). Tetramethylpyrazine significantly increased erythrocyte deformability index (P < 0.05) and certainly but not significantly improved blood viscosity at 14 days after spinal cord injury (P > 0.05). Tetramethylpyrazine significantly improved whole blood viscosity (hyposhear) and plasma viscosity at 28 days after spinal cord injury (P < 0.05). To conclude, after spinal cord injury, the whole blood viscosity and plasma viscosity can increase and erythrocyte indexes can also change to varying degrees. The use of tetramethylpyrazine can improve the deterioration of early hemorheological indexes and improve microcirculation disorders.

Key words: tetramethylpyrazine, spinal cord injury, hemorheology, microcirculation disorder, angiogenesis, endothelial cell

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