In vitro corrosion and in vivo biosafety of pure magnesium film

doi:10.3969/j.issn.2095-4344.2253

Abstract

Abstract:

BACKGROUND: Magnesium and its alloys hold good mechanical properties, biocompatibility and degradability, which can be clinically used as the membrane guiding bone regeneration in guided bone regeneration technology.

OBJECTIVVE: To explore the in vitro corrosion and in vivo biosafety of biodegradable high-purity magnesium film.

METHODS: The pure magnesium sample and the pure titanium sample were respectively immersed in artificial saliva. After 7 and 14 days, the sample was taken out to observe the surface corrosion, the weight loss rate and the average corrosion rate of the sample, and the pH value in the artificial saliva were determined. Six healthy mongrel dogs (provided by the Experimental Animal Center of Jinzhou Medical University) were selected to establish a bilateral mandibular defect model. The left side was implanted with pure magnesium film and the right side was implanted with titanium film. At 12 weeks postoperatively, specimens of the mandibular experimental area were removed for gross observation and X-ray examination. The histopathological observation of major organs was performed at the same time. The study was approved by the Ethics Committee of Jinzhou Medical University.

RESULTS AND CONCLUSION: (1) In vitro immersion test: pitting corrosion occurred on the surface of pure magnesium sample. Corrosion degradation became more and more serious with the immersion time. After 14 days of immersion, it showed surface corrosion. Pure titanium film showed no obvious corrosion. The pH value of the soaked pure magnesium film solution increased continuously, and it basically became balanced after 7 days, and remained at about 10. The pH value of pure titanium film was relatively stable, and slightly lower than that of pure magnesium film, and maintained at about 8.6. The weight loss rate of pure magnesium film immersed for 14 days was higher than that at 7 days (P < 0.05), and the average corrosion rate was lower than that of 7 days (P < 0.05). There was no obvious degradation behavior of pure titanium film, and no quality loss at each time point. (2) In vivo implantation experiment: film material in the pure magnesium film group was completely degraded, the trabecular bone arranged closely which formed a three-dimensional network structure, and some bone tissues were reconstructed into mature bones, but bone defects still existed. In the pure titanium film group, the defect area was filled with newly born bone tissue, the number of new trabecular bone was increased, and the trabecular bone in an order and tight arrangement. The bone volume fraction and bone pore volume fraction of the bone defect zone in the pure magnesium film group were significantly higher than those in the pure titanium film group (P < 0.05). There were no abnormalities in the liver and kidney structure of the experimental dogs, and no inflammatory cell infiltration was observed. (3) These results suggest that pure magnesium film can promote the bone healing in canine mandibular bone defect area, and the biosafety is good, but its corrosion resistance needs to be further improved.

Key words: pure magnesium film, pure titanium film, biosafety, in vitro corrosion, guided bone regeneration technique, mandibular defect, biocompatibility, in vivo experiment, bone defect repair

CLC Number:

Sun Xirao, Wang Chengyue, Zhao Yuan, Zhang Zhenbao. In vitro corrosion and in vivo biosafety of pure magnesium film[J]. Chinese Journal of Tissue Engineering Research, 2020, 24(16): 2578-2584.

Figures/Tables 6

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