Table of Content

01 January 2013, Volume 17 Issue 1 Previous Issue    Next Issue

For Selected:

Download Citations EndNote Reference Manager ProCite BibTeX RefWorks

Toggle Thumbnails

Select

Effect of activated autologous platelet-rich plasma on chondrogenic differentiation of rabbit bone marrow-derived mesenchymal stem cells in vitro Wang Shan-zheng, Wang Chen, Rui Yun-feng 2013, 17 (1):  1-8.  doi: 10.3969/j.issn.2095-4344.2013.01.001 Abstract ( 427 )   PDF (604KB) ( 631 )   Save BACKGROUND: Platelet-rich plasma, when activated, could secret multiple growth factors which may promote the proliferation and differentiation of bone marrow-derived mesenchymal stem cells. OBJECTIVE: To explore the effect of activated autologous platelet-rich plasma on the chondrogenic differentiation of rabbit bone marrow-derived mesenchymal stem cells in vitro. METHODS: Rabbit bone marrow-derived mesenchymal stem cells were isolated using the whole bone marrow adherence method. Passage 3 bone marrow-derived mesenchymal stem cells were in vitro cultured with 10% activated autologous platelet-rich plasma and 10% fetal bovine serum separately. Chondrogenic differentiation of rabbit bone marrow-derived mesenchymal stem cells in vitro was observed. RESULTS AND CONCLUSION: Bone marrow-derived mesenchymal stem cells were successfully isolated and exhibited a long-shuttle-shaped appearance. Cells grew faster when passaged. Immunofluorescence staining showed that after induced by activated autologous platelet-rich plasma, bone marrow-derived mesenchymal stem cells exhibited type Ⅱ collagen expression. Quantitative real-time PCR analysis showed that the expression of α1 chain of type Ⅱ collagen and aggrecan mRNA was significantly up regulated in the bone marrow-derived mesenchymal stem cells induced by activated autologous platelet-rich plasma than in the cells induced by fetal bovine serum (P < 0.01). Our findings in this study suggested that activated autologous platelet-rich plasma can promote the chondrogenic differentiation of rabbit bone marrow-derived mesenchymal stem cells in vitro. Figures and Tables | References | Related Articles | Metrics

Select

Differentiation of bone marrow mesenchymal stem cells into cardiomyocyte-likecells induced by P53 specific inhibitor p-fifty three inhibitor-alpha Yan Xue-bo, Hu Zhao-hui, Du Yun-hua, Lü An-lin, Xing Yu-jie 2013, 17 (1):  9-16.  doi: 10.3969/j.issn.2095-4344.2013.01.002 Abstract ( 301 )   PDF (435KB) ( 572 )   Save BACKGROUND: Latest researches have shown that P53 can significantly increase the differentiation rate of stem cells by locking the protein pathway P53-P21. While 5-azacytidine can inhibit cell proliferation and lead to apoptosis by activating the P53-P21 protein pathway.  OBJECTIVE: To investigate the effects of P53 specific inhibitor p-fifty three inhibitor-α on differentiation of rat bone marrow mesenchymal stem cells into cardiomyocyte-like cells. METHODS: Bone marrow mesenchymal stem cells were separated from Sprague-Dawley rats for culturing and passage. Passage 4 bone marrow mesenchymal stem cells were divided into four groups: normal control group, p-fifty three inhibitor-α group, 5-azacytidine and p-fifty three inhibitor-α+5-azacytidine group. RESULTS AND CONCLUSION: Primary bone marrow mesenchymal stem cells were smaller, and after passaged and induced, the cells were larger than primary cells and exhibited spindle-shaped appearance and arranged in order. MTT assay showed that p-fifty three inhibitor-α at 0-20 μmol/L could reduce the apoptosis of bone marrow mesenchymal stem cells. Identification results of cardiomyocyte-like cells showed that after induced for 4 weeks, little expression of cardiac troponin Ⅰ and CX-43 could be seen in the normal control group, and the expression of cardiac troponin Ⅰ and CX-43 in the other three groups was strong. After induced for 4 weeks, the differentiation rate of cardiomyocyte-like cells in the p-fifty three inhibitor-α and p-fifty three inhibitor-α+ 5-azacytidine group was significantly higher than that in the 5-azacytidine group. Western blotting analysis showed that at 1 week after induction, the expression of P53 and P21 in 5-azacytidine group was strongest compared with the other groups, but no expression was observed in p-fifty three inhibitor-α group. At 4 weeks after induction, the expression levels of P53 and P21 in 5-azacytidine group, p-fifty three inhibitor-α group and p-fifty three inhibitor-α+5-azacytidine group were higher than those in the normal control group. The expression levels of P53 and P21 in p-fifty three inhibitor-α group and p-fifty three inhibitor-α+5-azacytidine group at 4 weeks after induction were higher than those at 1 week after induction. It indicates that P53 specific inhibitor p-fifty three inhibitor-α can significantly induce the apoptosis, promote the proliferation of bone marrow mesenchymal stem cells through blocking P53-P21 protein pathway, and inhibit bone marrow mesenchymal stem cells to differentiate into cardiomyocyte-like cells. Figures and Tables | References | Related Articles | Metrics

Select

In vitro induced differentiation of bone marrow mesenchymal stem cells into bile duct epithelial-like cells Zhang Cheng, Yang Yu-long, Lin Mei-ju, Shi Li-jun, Zhang Hong-wei, Li Jing-yi 2013, 17 (1):  17-22.  doi: 10.3969/j.issn.2095-4344.2013.01.003 Abstract ( 353 )   PDF (423KB) ( 615 )   Save BACKGROUND: Extrahepatic bile duct and gallbladder epithelial cells are easily isolated and purified, but bile duct epithelial cells predispose to lose proliferative capacity under in vitro circumstances. Thus, enough amounts of cells used for basic studies are hardly acquired, which limits the progression of bile duct repair. Bone marrow mesenchymal stem cells can be differentiated into hepatocytes, but there have been no reports regarding in vitro induced differentiation of bone marrow mesenchymal stem cells into bile duct epithelial cells.  OBJECTIVE: To discuss the feasibility of inducing bone marrow mesenchymal stem cells into the bile duct epithelial cells in vitro, and to find suitable seed cells for the repair of bile duct injury.  METHODS: Rat bone marrow mesenchymal stem cells were in vitro isolated and purified by whole bone marrow adherent screening in vitro. Passage 3 bone marrow mesenchymal stem cells were cultured with culture medium containing hepatocyte growth factor and epidermal growth factor. The morphological changes of bone marrow mesenchymal stem cells were observed under the inverted microscope and CK19 expression was detected by immunofluorescence assay at different time periods. RESULTS AND CONCLUSION: Under the induction by hepatocyte growth factor and epidermal growth factor, bone marrow mesenchymal stem cells gradually appeared from shuttle-shaped to polygonal or triangular. Immunofluorescence examination showed that CK19 expression appeared in the cell membrane in the 4th week of induction and it was significantly increased in the 6th week. These findings suggest that bone marrow mesenchymal stem cells can be induced to differentiate into duct epithelial-like cells under the combined induction of hepatocyte growth factor and epidermal growth factor, which provides a new thought for bile duct repair with bone marrow mesenchymal stem cells. Key Words: stem cells; bone marrow-derived stem cells; bone marrow mesenchymal stem cells; hepatocyte growth factor; epidermal growth factor; bile duct epithelial cells; CK19; stem cell photographs-containing paper Figures and Tables | References | Related Articles | Metrics

Select

Treatment of traumatic brain injury in rats by RhoA gene silencing combinedwith umbilical cord mesenchymal stem cell transplantation Feng Shi-jun, Han Jian-guo 2013, 17 (1):  23-30.  doi: 10.3969/j.issn.2095-4344.2013.01.004 Abstract ( 395 )   PDF (409KB) ( 580 )   Save BACKGROUND: Several studies have demonstrated that Rho kinase can lead to collapse of nerve growth cone and exhibits an inhibitory effect on nerve repair. OBJECTIVE: To investigate the effects of human umbilical cord mesenchymal stem cells combined with RNAi-mediated RhoA gene silencing on recovery of traumatic brain injury in rats. METHODS: Eighty-four healthy Wistar rats were prepared into models of traumatic brain injury by 253.312 5- 303.975 kPa hydraulic pressure impact force. Then traumatic brain injury models were randomly divided into control group, umbilical cord mesenchymal stem cell transplantation group and umbilical cord mesenchymal stem cell transplantation+RhoA gene silencing group (combination group). After transplantation of CM-Dil-labeled umbilical cord mesenchymal stem cells, rat neurological function was evaluated through the use of a modified neurological severity scores. At 21-28 days after traumatic brain injury development, the Morris water maze test was performed. After 4 weeks, following sacrifice, the whole rat brain was frozen, sliced, stained with hematoxylin-eosin and finally the survival and distribution of CM-Dil-labeled umbilical cord mesenchymal stem cells were observed under fluorescent microscope. RhoA gene expression in the injured region in each group was detected using RT-PCR. RESULTS AND CONCLUSION: At 1, 2, 3 and 4 weeks after traumatic brain injury, the modified neurological severity scores were significantly lower in the umbilical cord mesenchymal stem cell transplantation group than those in the control group (P < 0.05), and the scores were significantly lower in the combination group than those in the control group (P < 0.01). Morris water maze test results showed that the mean escape latency was gradually shortened in each group. The mean escape latency across 3-5 days after traumatic brain injury development in the combination group was significantly shorter than that in the umbilical cord mesenchymal stem cell transplantation group (P < 0.05) and in the control group (P < 0.01). The number that rats crossed the platform and the percentage of swimming distance in the target quadrant to total distance were significantly higher in the combination group compared to the control group and the umbilical cord mesenchymal stem cell transplantation group (P < 0.05). At 4 weeks after cell transplantation group, the number of neurons and CM-Dil-positive cells in the umbilical cord mesenchymal stem cell transplantation group was significantly lower compared to the combination group but it was significantly higher compared to the control group (both P < 0.05). RhoA mRNA expression in the combination group was significantly lower compared to the control group and umbilical cord mesenchymal stem cell transplantation group (P < 0.05). These findings suggest that umbilical cord mesenchymal stem cell transplantation can greatly improve the neurological function of rats with traumatic brain injury, and it exhibits better effects after combined with RhoA gene silencing. Figures and Tables | References | Related Articles | Metrics

Select

Expression of lentiviral vectors mediated NEP1-40 in mesenchymal stem cells fromhuman umbilical cord blood Huang Ai-hua, Zhou Yi-dan, Zhang Ping-ping, Wang Sai-ying, Lin Yuan-yuan, Ge Ting-ai, Chen Zan 2013, 17 (1):  31-37.  doi: 10.3969/j.issn.2095-4344.2013.01.005 Abstract ( 357 )   PDF (750KB) ( 609 )   Save BACKGROUND: In recent years, studies have shown that mesenchymal stem cells are ideal seed cells used fortissue engineering because of their strong proliferation and multi-differentiation potential. Mesenchymal stem cells can be efficiently transfected and expressed exogenous gene, and therefore they have broad application prospects in gene therapy. OBJECTIVE: NEP1-40 gene-containing lentiviral vectors were transfected into umbilical cord blood-derived mesenchymal stem cells to evaluate the biological function changes of mesenchymal stem cells and detect NEP1-40 expression in umbilical cord blood-derived mesenchymal stem cells. METHODS: Human umbilical cord blood-derived mesenchymal stem cells were isolated and cultured in vitro. Cell surface markers were detected by flow cytometry, and their biological characteristics were identified. NEP1-40 gene was cloned into the lentiviral vector and lentiviral supernatant was packaged. Then umbilical cord blood-derived mesenchymal stem cells were transfected with different multiplicities of infection. RESULTS AND CONCLUSIONS: We successfully isolated and cultured human umbilical cord blood-derived mesenchymal stem cells in vitro by density gradient centrifugation method, and the cells could be induced to differentiate into adipocytes. Flow cytometry results showed that umbilical cord blood-derived mesenchymal stem cells were positive for CD90, CD73 and CD105 protein, but they were negative for CD14, CD34, CD45, CD19, HLA-DR, Stro-1 and CD106 protein. Real-time PCR detection showed that the NEP1-40 mRNA expression level was positively correlated with multiplicity of infection. Higher multiplicity of infection yielded higher NEP1-40 expression. In addition, NEP1-40 protein expression could be seen in human umbilical cord blood-derived mesenchymal stem cells after transfected with NEP1-40. These findings suggest that the transfection of NEP1-40 gene has little impact on biological function of human umbilical cord blood-derived mesenchymal stem cells. Figures and Tables | References | Related Articles | Metrics

Select

Effect of different anesthetic conditions on proliferation and adipogenic differentiation of human adipose-derived stem cells Wu Li-xin, Li Hong-mian, Liu Da-lie, Nan Hua 2013, 17 (1):  38-43.  doi: 10.3969/j.issn.2095-4344.2013.01.006 Abstract ( 318 )   PDF (374KB) ( 556 )   Save BACKGROUND: Few reports have reported the effects of anesthetic with different ingredients and concentrations on the biological characteristics of adipocyte and human adipose-derived stem cells, and the negative impacts such as toxic reaction are still unclear. OBJECTIVE: To investigate the effects of different anesthetic conditions on the proliferation and adipogenic differentiation of human adipose-derived stem cells in vitro in order to reduce anesthetic-induced damage and increase the availability of human adipose-derived stem cells. METHODS: Human adipose-derived stem cells were isolated from the subcutaneous adipose tissue of healthy adults after liposuction, and primary culture and subculture of human adipose-derived stem cells were conducted. Passage 3 human adipose-derived stem cells were treated with 0.2 g/L of final concentration medium which containing lidocaine, ropivacaine hydrochloride and bupivacaine, respectively. A control group was designated. The damage condition, growth and proliferation of human adipose-derived stem cells were analyzed and the adipogenic differentiation ratio was compared. RESULTS AND CONCLUSION: Compared with the control group, the lactate dehydrogenase level of cell supernatants in lidocaine group, ropivacaine group and bupivacaine group at all time points was significantly increased, the absorbance value was decreased, and the difference was significant (P < 0.05); furthermore, there was significant difference in the lactate dehydrogenase level of cell supernatants at all time points between lidocaine group and ropivacaine group or bupivacaine group (P < 0.05). There was no significant difference of adipogenic differentiation ratio of human adipose-derived stem cells between the four groups (P > 0.05). The medium containing lidocaine, ropivacaine hydrochloride and bupivacaine at 0.2 g/L final concentration exhibits the growth and proliferation of human adipose-derived stem cells, but it has no influence on the adipogenic differentiation of human adipose-derived stem cells. Figures and Tables | References | Related Articles | Metrics

Select

Adipose-derived stromal cells differentiate into cholinergic neurons Deng Chun-ying, Liu Bin, Zhang Zhi-yong, Zhang Yu-qin, Li Yan, Yi Hong-li 2013, 17 (1):  44-49.  doi: 10.3969/j.issn.2095-4344.2013.01.007 Abstract ( 438 )   PDF (372KB) ( 522 )   Save BACKGROUND: Adipose-derived stromal cells have multilineage differentiation potential, and it can differentiate into mesodermal cells and endodermal and ectodermal cells. Under certain conditions, they can differentiate into cholinergic neurons. OBJECTIVE: To explore in vitro culture of adipose-derived stromal cells and their ability to differentiate into cholinergic neurons. METHODS: Subcutaneous inguinal fat pads from 2-4 week-old Sprague-Dawley rats were taken and then in    vitro isolated, cultured and passaged. After induced and cultured with neural induction medium, CD29, CD31 and CD44 expressions was detected by immunocytochemical tests and the phenotypic identification was performed. RESULTS AND CONCLUSION: During the in vitro culture, adipose-derived stromal cells adhered to culture flask wall, proliferated strongly, and they were positive for CD29 and CD44, but negative for CD31. After induced and cultured with neural induction medium, the differentiated cells showed a typical neuron-like cells morphology, and the expression of nestin, neuron-specific enolase and microtubule-associated protein 2 was positive. The expression of choline acetyltransferase was positive after induction and differentiation. It indicates that adipose-derived stromal cells can differentiate into neuron-like cells and the differentiated cells have the function of synthesizing choline acetyltransferase. Figures and Tables | References | Related Articles | Metrics

Select

Establishment of fumarylacetoacetate hydrolase gene knockout rat embryonic stem cell strains Ye Zhen-long, Wang Yan, Jin Hua-jun, Liu Tao, Qian Qi-jun 2013, 17 (1):  50-55.  doi: 10.3969/j.issn.2095-4344.2013.01.008 Abstract ( 404 )   PDF (430KB) ( 787 )   Save BACKGROUND: Mouse liver injury model has been widely used. The pathological mechanism of liver injury and the environment of liver transplantation in rats can be used for simulation in humans. Establishing fuarylacetoacetat hydrolase gene knockout embryonic stem cell strains is the key to establish a rat model of liver injury. OBJECTIVE: To establish fuarylacetoacetat hydrolase gene knockout rat embryonic stem cell strains. METHODS: We first constructed the targeting vector in vitro with positive-negative selective cassette, then transfered the linearized vector into embryonic stem cells through electroporation. We used embryonic stem cell culture medium containing 0.5 μg/mL puromycin to select puromycin resistant clones 36 hours after electroporation, and then we performed PCR to confirm targeted embryonic stem cell clones.  RESULTS AND CONCLUSION: After electroporation, about 90% of embryonic stem cell clones expressed green fluorescence. Two embryonic stem cell clones expressing green fluorescence were selected and confirmed by PCR. The fuarylacetoacetat hydrolase gene deficient rat embryonic stem cell strains were successfully established. This provides evidence for later establishing gene knockout animal models. Figures and Tables | References | Related Articles | Metrics

Select

In vitro isolation, culture and identification of brain tumor stem cells from human astrogliomas Song Xiao-bin, Yang Zhi-yong, Deng Xing-li, Wang Xiang-peng, Zhang Sheng-ping, Huang Jin, Zhang Xing-kui 2013, 17 (1):  56-61.  doi: 10.3969/j.issn.2095-4344.2013.01.009 Abstract ( 419 )   PDF (567KB) ( 725 )   Save BACKGROUND: According to the theory of tumor stem cells, there are a small amount of stem cell-like cells that exhibit infinite proliferative potential and self-renewal capacity, can differentiate into cells with the phenotype of mature cells and play a key role in tumor production, proliferation and invasion. OBJECTIVE: To investigate the feasibility of isolation, culture and identification of brain tumor stem cells from human astrogliomas. METHODS: Brain tumor stem cells were isolated by primary culture from human astrogliomas. These cells were cultured under the culture condition of neural stem cells. The clone spheres were identified with immunocytochemistery for nestin and CD133. At the same time, differentiated cells were identified by immunocytochemistery for neuron specific enolase, glial fibrillary acidic protein and O4, respectively. RESULTS AND CONCLUSION: After 7-10 days of culture, a great number of neurospheres immunoreactive for nestin and CD133 were observed. After induced differentiation, these cells were immunoreactive for neuron specific enolase, glial fibrillary acidic protein and O4. These findings suggest that there are brain tumor stem cells with the characteristics of neural stem cells in human astrogliomas. CD133 and nestin are key surface markers for brain tumor stem cells, which can be used for isolation of brain tumor stem cells. Figures and Tables | References | Related Articles | Metrics

Select

Isolation, culture and cell viability testing of rabbit bone marrow mesenchymal stem cells Kong Gen-xian, Jiang Zhi-xin, Sha Hang, Zhang Qing-hua 2013, 17 (1):  62-67.  doi: 10.3969/j.issn.2095-4344.2013.01.010 Abstract ( 391 )   PDF (433KB) ( 554 )   Save BACKGROUND: Most of recent studies on isolation and purification of bone marrow mesenchymal stem cells adopt single methods, and there is no systematic and comprehensive evaluation on cell viabilities during amplification. OBJECTIVE: To establish an effective method of purifying rabbit bone marrow mesenchymal stem cells and to test the cell viabilities.    METHODS: Rabbit bone marrow mesenchymal stem cells were isolated by density gradient centrifugation and adherent cultivation methods. Rabbit bone marrow mesenchymal stem cells were identified from the morphology, cell phenotype and osteogenic and adipogenic capacity. Cell viabilities were evaluated using growth curve, seeding efficiency and colony-forming efficiency. RESULTS AND CONCLUSION: The primary cells were spindle-shaped and circular. Most of the cells attached to the dish after seeding for 48 hours. After 8 or 10 days, cells reached 80%-90% confluency and the subculture cycle was 3 to 5 days. Flow cytometry demonstrated that cells were negative for CD14, CD34 and CD45, but they were positive for CD29, CD44 and CD90. Calcium nodules were observed by alizarin red S staining. A large amount of adipose-derived stem cells were observed by oil red O staining. Cell viability of passages 1 and 3 was stronger than that of passage 5. These findings suggest that density gradient centrifugation combined with adherent cultivation can be used to effectively isolate and purify bone marrow mesenchymal stem cells and well maintain the proliferation and differentiation potential of bone marrow mesenchymal stem cells. Cell viability will be decreased to different extents during cell proliferation. Key Words: stem cells; bone marrow-derived stem cells; bone marrow mesenchymal stem cells; isolation and purification; identification; growth curve; adherence rate; clone formation rate; National Natural Science Foundation of China; stem cell photographs-containing paper Figures and Tables | References | Related Articles | Metrics

Select

Transplantation of controlled release glial cell line-derived neurotrophic factor and bone marrow mesenchymal stem cells-derived neuron-like cells reduces myelosyringosis after spinal cord injury Liu Xiao-gang, Deng Yu-bin, Cai Hui, Wang Li-ning, Ma Yu-lin, Zhang Xin-peng, Wei Ke-xin 2013, 17 (1):  68-73.  doi: 10.3969/j.issn.2095-4344.2013.01.011 Abstract ( 318 )   PDF (411KB) ( 576 )   Save BACKGROUND: Previous studies have demonstrated that transplantation of controlled release glial cell line-derived neurotrophic factor (GDNF) and bone marrow mesenchymal stem cells-derived neuron-like cells (BMSCs) can effectively promote the motor function recovery of rhesus monkeys with spinal cord injury. OBJECTIVE: To validate whether GDNF+BMSCs transplantation exhibits better protective effects on spinal cord tissue of rhesus monkeys with spinal cord injury than BMSCs transplantation. METHODS: Rhesus monkey models of acute severe spinal cord injury were divided into three groups and received GDNF+BMSCs transplantation (GDNF+BMSCs group) or BMSCs transplantation (BMSCs group) or PBS administration (control group). Spinal cord tissue was embedded with paraffin and stained with hematoxylin-eosin. Myelosyringosis was estimated by image analysis system. RESULTS: In the control group, spinal cord structure was greatly destructed, and myelosyringosis occupied larger area. In the BMSCs group, spinal cord structure was well preserved, and small cavities, occasionally large cavities, were observed. In the GDNF+BMSCs group, spinal cord structure was best preserved and only small cavities were observed. There was significant difference in spinal cord structure destruction between groups (P < 0.01). These findings suggest that compared with simple BMSCs transplantation, transplantation of controlled released GDNF+BMSCs shows better protective effects on spinal tissue structure after spinal cord injury, which may be one of mechanisms by which myelosyringosis is reduced to a greater extent. Figures and Tables | References | Related Articles | Metrics

Select

Subarachnoid space transplantation of superparamagnetic iron oxide-labeled bone marrow mesenchymal stem cells for treatment of spinal cord injury Zhang Rui-ping, Li Jian-ding, Liu Qiang, Shuang Wei-bing, Xie Jun 2013, 17 (1):  74-78.  doi: 10.3969/j.issn.2095-4344.2013.01.012 Abstract ( 262 )   PDF (309KB) ( 556 )   Save BACKGROUND: Transplantation of stem cells can rebuild the structure and function of injured central nervous system, and has attracted wide attention in recent years. OBJECTIVE: To explore the effects of bone marrow mesenchymal stem cells labeled by superparamagnetic iron oxide on the recovery of neurological function in rabbits with spinal cord injury. METHODS: Bone marrow mesenchymal stem cells were isolated from rabbits, and cultivated in vitro using the density gradient centrifugation and the adherence in vitro separation. Passage 3 bone marrow mesenchymal stem cells were labeled with superparamagnetic iron oxide at 24 hours before use. Rabbit spinal cord injury models were made and the micro tubes were inserted into subarachnoid space. Rabbits were randomly divided into three groups．Rabbits from the group A were injected with superparamagnetic iron oxide labeled bone marrow mesenchymal stem cells via subarachnoid spaces. Rabbits from the group B received transplan tation of unlabeled bone marrow mesenchymal stem cells. Rabbits from the group C were injected with PBS and served as controls. At 1, 7, 14, 21, 28 and 35 days after cell transplantation, the recovery of neurological function was calculated by BBB scoring in all groups and the pathological tissue slices of spinal cord injury was examined. RESULTS AND CONCLUSION: The BBB scores in the groups A and V were significantly higher than those in the group C (P < 0.05), but there was no statistical difference between groups A and B (P > 0.05). At 1, 7, 14, 21, 28 and 35 days following cell transplantation, Prussian blue staining of tissue sections showed cells containing blue iron particles in the area of spinal cord injury. Bone marrow mesenchymal stem cells transplanted via subarachnoid space could migrate to the area of spinal cord injury, which thereby improved neurological function. Figures and Tables | References | Related Articles | Metrics

Select

Proliferation and differentiation of neural stem cells from newborn mouse hippocampi in vitro Hu Ying-ying, Duan Xian-hua, Chen Mei, Wang De-guang 2013, 17 (1):  79-85.  doi: 10.3969/j.issn.2095-4344.2013.01.013 Abstract ( 452 )   PDF (367KB) ( 838 )   Save BACKGROUND: Neural stem cells transplantation can be used for clinical therapy of central nervous system injury. OBJECTIVE: To observe the characteristics of proliferation and differentiation of neural stem cells cultured in vitro. METHODS: Neural stem cells were isolated mechanically from newborn Kunming mouse hippocampus and cultured with serum-free cell culture medium. They were obtained by mechanical dissociation and enzymatic digestion. The neurospheres were identified by immunofluorescent staining of Nestin. The differentiation of neural stem cells was induced by 10% fetal bovine serum and identified by immunohistochemistry of β-Ⅲ-tubulin and glial fibrillary acidic protein. CCK-8 assay was used to ascertain the capacity for neural stem cell proliferation. RESULTS AND CONCLUSION: The neural stem cells obtained from newborn mouse hippocampus had the capacity for forming the neurospheres which were positive for Nestin. The differentiated cells induced by 10% fetal bovine serum expressed β-Ⅲ-tubulin or glial fibrillary acidic protein. The method of isolation  and culture of neural stem cells from newborn mouse hippocampus in vitro was successfully established. Neural stem cells have the capacity for self-renewal and the potential for differentiation. Figures and Tables | References | Related Articles | Metrics

Select

Generation of induced pluripotent stem cells from villus cells after induction by four kinds of genes during prenatal diagnosis Luo Yu-mei, Fan Yong, Chen Xin-jie, Yue Lei, Li Qing, Yu Bo-lan, He Wen-zhi, Ma Xiao-yan, Sun Xiao-fang 2013, 17 (1):  86-91.  doi: 10.3969/j.issn.2095-4344.2013.01.014 Abstract ( 297 )   PDF (448KB) ( 626 )   Save BACKGROUND: Due to pluripotent characteristics, induced pluripotent stem cells can be induced to differentiate into the specific cells, including nerve cells and hematopoietic cells. OBJECTIVE: To establish the induced pluripotent stem cells generated from villus cells during prenatal diagnosis. METHODS: The prenatal diagnosis villus cells were induced by retroviral vectors encoding hOct4, hSox2, hKlf4 and hc-Myc. The pluripotency, differentiation ability and karyotype of the induced pluripotent stem cells were detected. RESULTS AND CONCLUSION: The induced pluripotent stem cells maintained the self-renewal ability with high pluripotent marker gene and protein expression and had in vivo and in vitro differentiation potential. The induced pluripotent stem cells could maintain normal karyotype in vitro after long-term culture. The induced pluripotent stem cells obtained from the prenatal diagnosis villus cells provide an ideal source for autologous cell-replacement therapy in the later life of the fetus, and can be used as a perfect model for the research of mechanism underlying prenatally diagnosed diseases. Figures and Tables | References | Related Articles | Metrics

Select

Effects of drynaria total flavonoid on osteogenic differentiation of rat dental pulp stem cells via PI3K/Akt pathway Huang Xiao-fei, Yuan Su-jian, Yang Cheng 2013, 17 (1):  92-97.  doi: 10.3969/j.issn.2095-4344.2013.01.015 Abstract ( 352 )   PDF (373KB) ( 618 )   Save BACKGROUND: PI3K/Akt pathway may play an important regulative role in osteogenic differentiation of dental pulp stem cells induced by drynaria total flavonoid.   OBJECTIVE: To explore the effects of total flavonoids of drynaria on osteogenic differentiation of rat dental pulp stem cells and investigate the role of PI3K/Akt pathway in the induced differentiation of rat dental pulp stem cells. METHODS: Rat dental pulp stem cells were harvested by enzymatic digestion, cultured and identified by immunohistochemical staining. The dental pulp stem cells were cultured in media with different concentrations of drynaria total flavonoid (0.01 g/L, 0.05 g/L and 0.1 g/L). Alkaline phosphatase activities and formation of calcified tubercles in cells were determined in each group. Phosphorylated Akt expression in cells was detected by western blot method in each group. RESULTS AND CONCLUSION: Dental pulp stem cells were positive for CD44 and CD29 expression, but they were negative for CD34. Compared to the blank control group, alkaline phosphatase activities, formation of calcified tubercles and phosphorylated Akt expression were significantly increased in the drynaria total flavonoid group in a dose- and time-dependent manner. These findings suggest that drynaria total flavonoid can promote the osteogenic differentiation of rat dental pulp stem cells, and this effect is likely mediated by PI3K/Akt pathway. Figures and Tables | References | Related Articles | Metrics

Select

Effects of eupolyphaga-containing serum on proteomics changes in bone marrow mesenchymal stem cells Zhong Wei-hong, Qi Zhen-xi2 2013, 17 (1):  98-105.  doi: 10.3969/j.issn.2095-4344.2013.01.016 Abstract ( 338 )   PDF (330KB) ( 580 )   Save BACKGROUND: Avascular necrosis of the femoral head has been much studied. However, the precise pathological mechanism underlying avascular necrosis of the femoral head remains unclear. OBJECTIVE: To investigate the effects of eupolyphaga-containing serum on the target or related proteins inhibiting adipogenic differentiation and promoting osteogenic differentiation of bone marrow mesenchymal stem cells.      METHODS: Passage 3 bone marrow mesenchymal stem cells were cultured with DMEM containing blank serum (blank control group), dexamethasone (hormone group) and eupolyphaga-containing serum (eupolyphaga group). After 6 days of culture, isobaric tag for relative and absolute quantitation technique was used to quantify proteins from three groups. The proteins were identified and relatively quantitatively analyzed by mass spectrometry. RESULTS AND CONCLUSION: Relative to the hormone group, there were 15 proteins significantly up regulated and 12 proteins significantly down regulated in the blank control group, and there were 16 proteins significantly up regulated and 14 proteins significantly down regulated in the eupolyphaga group. Hspa1L1 and PrxⅤ may be the targets of anti-apoptosis in eupolyphaga. The expression of Serpinh1 and ADP/ATP translocase 1 may be the targets of promoting osteogenic differentiation in eupolyphaga. Figures and Tables | References | Related Articles | Metrics

Select

Hypoxia-inducible factor-1α gene transfected adipose-derived stem cells promote the survival of autologous fat grafts Wang He-geng, Li Hong-mian, Liu Da-lie, Nan Hua, Zhao Pei-ran, Liang Shuang-wu 2013, 17 (1):  106-111.  doi: 10.3969/j.issn.2095-4344.2013.01.017 Abstract ( 347 )   PDF (457KB) ( 540 )   Save BACKGROUND: Autologous fat is an ideal filling material in aesthetic and reconstructive surgery. However, the long-term effects are poor because much tissue is absorbed after grafting.  OBJECTIVE: To investigate the effects of hypoxia-inducible factor-1α gene transfected adipose-derived stem cells on survival of autologous fat grafts. METHODS: Adipose-derived stem cells were isolated from the subcutaneous adipose tissue of healthy adults after liposuction, primary cultured and sub-cultured. Passage 3 adipose-derived stem cells at a density of 1×   109 cells/L were transfected with hypoxia-inducible factor-1α gene. The cells were then adjusted to 1×1011 cells/L. Simultaneously, autologous fat from the same liposuction was selected and purified. The grafts were made according to physiological characteristics of fibrin glue. Three groups were designated. In the gene-modified group, hypoxia-inducible factor-1α gene-modified adipose-derived stem cells, autologous fat+fibrin glue were transplanted into the back of nude mice. In the non-gene-modified group, adipose-derived stem cells+autologous fat+fibrin glue were transplanted in the identical region. In the saline group, physiological saline+autologous fat+ fibrin glue were transplanted. RESULTS AND CONCLUSION: At 3 and 6 months after transplantation, the vascular density in the graft was highest in the gene-modified group, followed by non-gene-modified group, and lastly the saline group and there was significant difference between groups (P < 0.05). Fiber necrosis rate of adipose-derived stem cells was lowest in the gene-modified group, followed by non-gene-modified group, and lastly the saline group and there was significant difference between groups (P < 0.05). Fat maintenance rate was highest in the gene-modified group, followed by non-gene-modified group, and lastly the saline group and there was significant difference between groups (P < 0.05). These findings suggest that hypoxia-inducible factor-1α gene transfected adipose-derived stem cells can accelerate the vascularization in the local fat graft transplanted, promote the survival of adipose cells, increase the mass maintenance rate of fat graft and lower the fibrosis necrosis degree after fat grafting. Figures and Tables | References | Related Articles | Metrics

Select

null Zhou Yan, Piao Jin-hua, Jin Lian-hua, Yang Si-rui 2013, 17 (1):  112-117.  doi: 10.3969/j.issn.2095-4344.2013.01.018 Abstract ( 641 )   PDF (424KB) ( 654 )   Save null Figures and Tables | References | Related Articles | Metrics

Select

Research progress in bone marrow mesenchymal stem cells for promotion of islet regeneration Fan Zi-yang, Bai Jin-bao, Wang Hui, Qie Hong-zheng, Deng Xin-sheng, Hu Ying, Liang Hai-sheng, 2013, 17 (1):  118-123.  doi: 10.3969/j.issn.2095-4344.2013.01.019 Abstract ( 272 )   PDF (403KB) ( 491 )   Save BACKGROUND: Bone marrow mesenchymal stem cells transplanted into diabetes mellitus rats can decrease glucose level. OBJECTIVE: To summarize the research progress in bone marrow mesenchymal stem cells for promotion of islet regeneration. METHODS: A computer-based online retrieval of PubMed and Wanfang databases was performed to search papers published during July 2003 to December 2011 with the key words bone marrow mesenchymal stem cells, islet cells in English and Chinese. Twenty-five papers were included in the final analysis. RESULTS AND CONCLUSION: At present, transplantation of islets has made great advance in treatment of diabetes mellitus. Many patients with diabetes mellitus hardly benefit from islet transplantation because of limited islet origins and the immunological rejections of heterogenous or xenogenous islets. Bone marrow mesenchymal stem cells are characterized by easy to operate, easy in vitro isolation, culture and purification, and multipotential differentiation. Islets induce-differentiated by bone marrow mesenchymal stem cells are expected to solve islet-related problems of the limited origin and immunological rejections. This paper summarizes the research progress in bone marrow mesenchymal stem cells for treatment of diabetes mellitus and points out the existing problems and further study direction. Figures and Tables | References | Related Articles | Metrics

Select

Bone marrow microenvironment and disease development Shao Xiao-hu, Le Huang-ying 2013, 17 (1):  124-130.  doi: 10.3969/j.issn.2095-4344.2013.01.020 Abstract ( 495 )   PDF (412KB) ( 480 )   Save BACKGROUND: Bone marrow microenvironment change is closely related to the occurrence and development of hematopoietic disease. OBJECTIVE: To review the cell and non-cell components in the bone marrow microenvironment as well as the regulatory role of signaling pathway and to investigate the relationship between bone marrow microenvironment change and disease development. METHODS: A computer-based online retrieval of Pubmed database and Wanfang database was performed using the English key words “bone marrow niche, hematopoietic stem cell” and Chinese key words “bone marrow microenvironment, hematopoietic stem cells” to search papers published between January 2001 and April 2012. A total of 424 papers were retrieved and 61 were suitable for final analysis. RESULTS AND CONCLUSION: The adult hematopoietic stem cells in bone marrow are the origins of blood and lymphocyte systems. Bone marrow microenvironment can support the self-renewal and differentiation of hematopoietic stem cells and it is composed of various kinds of hematopoietic cells, non-hematopoietic cells, extracellular matrix and other signal proteins. Normal bone marrow microenvironment plays an important role in sustaining the normal function of hematopoietic cells. Once the balance is broken, some malignant diseases such as leukemia and cancer would be caused. Therefore, further investigation of bone marrow microenvironment and understanding the regulatory mechanism and the determinative factor of stem cell fate will greatly facilitate bone marrow transplantation, tissue repair and regenerative medicine development. Figures and Tables | References | Related Articles | Metrics

Select

Hematopoietic stem cells transplantation for the treatment of refractory multiple myeloma Chen Yi,Yang Ze-song, Chen Jian-bin 2013, 17 (1):  131-136.  doi: 10.3969/j.issn.2095-4344.2013.01.021 Abstract ( 305 )   PDF (387KB) ( 571 )   Save BACKGROUND: In recent years, the morbidity of multiple myeloma is increased; however, this disease cannot be cured now. With the improvements in hematopoietic stem cells transplantation technology and clinical application of new drugs, as well as cell transplantation treatment combined with new drug application, multiple myeloma patients will have new hope in their lives. OBJECTIVE: To summarize the progress of refractory multiple myeloma treatment by hematopoietic stem cells transplantation combined with new drug therapy in recent years. METHODS: A computer-based search was performed on PubMed database, ISI platform BP+Medline database, Wanfang database and VIP database for articles describing hematopoietic stem cells transplantation and immunoregulant, protease inhibitors combined cell transplantation published during January 1990-April 2012. Articles with obsolete and repeated contents were rejected. RESULTS AND CONCLUSION: A total of 22 articles in Chinese and English were obtained after retrieval and screening. Analysis of retrieved articles shows that hematopoietic stem cells transplantation combined with new drug treatment strategy can greatly improve the effects of high-dose chemotherapy and autologous stem cell transplantation treatment. Pre-transplantation induction with new drug application and post-transplantation maintenance with new drug can delay the progression and enhance the overall survival rates. Allogeneic hematopoietic stem cell transplantation therapy will achieve long-term molecular biological relief and therapeutic effects in multiple myeloma patients. The combination of a variety of treatment methods and the comparison between the advantages and disadvantages can help the clinicians to make better decision-making in practice according to real situation of the patients, and can satisfy the needs of patients. Figures and Tables | References | Related Articles | Metrics

Select

Protective effects of traditional Chinese medicine on mesenchymal stem cells transplanted into the hippocampus of rats with post-traumatic stress disorder Fu Wen-jun, Ao Hai-qing, Zeng Lei, Xu Zhi-wei 2013, 17 (1):  137-141.  doi: 10.3969/j.issn.2095-4344.2013.01.022 Abstract ( 367 )   PDF (370KB) ( 446 )   Save BACKGROUND: Transplantation of bone marrow mesenchymal stem cells shows great potential in repair of hippocampal injury. However, low survival rate of transplanted stem cells remains unsolved. OBJECTIVE: To summarize the protective effects of traditional Chinese medicine on bone marrow mesenchymal stem cells transplanted in the hippocampus of rats with post-traumatic stress disorder. METHODS: The databases of Pubmed, and CNKI were retrieved for papers published from January 2000 to January 2012 with key words “mesenchymal stem cells, transplantation, survival and post-traumatic stress disorder (PTSD), hippocampus” in English and Chinese. A total of 109 papers were retrieved. Among them, 23 papers were included in the final analysis. RESULTS AND CONCLUSION: Post-traumatic stress disorder is a complex disease influenced by biological, psychological, social and other factors and remains unsolved in treatment. Traditional Chinese medicine exhibits a unique advantage in this research field. Hippocampal damage caused by post-traumatic stress disorder can be repaired by transplantation of bone marrow mesenchymal stem cells. Bone marrow mesenchymal stem cell transplantation combined with intervention of Xiaoyaosan, a traditional Chinese medicine, is expected to increase the survival rate of transplanted stem cells. Figures and Tables | References | Related Articles | Metrics

Select

Mesenchymal stem cells: Biological characteristics and therapeutic implications for cardiovascular diseases Chen Ting, Chen Guang-hui 2013, 17 (1):  142-149.  doi: 10.3969/j.issn.2095-4344.2013.01.023 Abstract ( 329 )   PDF (459KB) ( 581 )   Save BACKGROUND: The application of mesenchymal stem cells is an effective method for treatment of cardiac diseases, such as acute myocadial infarction and heart failure, owing to their self-renewal, multi-lineage differentiation and immunomodulation. OBJECTIVE: To summarize the current understanding of biological characteristics, translational findings, immunomodulation, mechanism underlying cardiac function repair of mesenchymal stem cells as well as clinical data regarding mesenchymal stem cells for early cardiovascular disease. METHODS: A computer-based online retrieval was performed to search papers in VIP periodical full-text database and PubMed database using the key words MSCs, immunomodulation, myocardial infarction, heart failure in Chinese and English. After excluding objective-independent papers, 35 papers were included for further analysis. RESULTS AND CONCLUSION: Mesenchymal stem cells are a kind of ideal cells with the capacity of self-renewal, multi-lineage differentiation, and immunomodulation, as well as being free of immunological rejection due to allotransplantation or xenotransplantation. Mesenchymal stem cellssecrete bioactive levels of soluble factors (growth factors and cytokines) by paracrine secretion and recruit endogenous cardiac stem cells. These characteristics make mesenchymal stem cells to differentiate into cardiomyocytes and promote angiogenesis, which can reverse remodeling and reduce the area of myocardial infarction. Recently, some related clinical trials also showed the feasibility and effectiveness of transplantation of mesenchymal stem cells, which provide a solid theoretical foundation for stem cells used in cardiovascular diseases. Figures and Tables | References | Related Articles | Metrics

Select

Research progress in stem cell transplantation for treatment of diabetic nephropathy Yang Xiao-yan, Pan Xing-hua, Ruan Guang-ping, Pang Rong-qing, Cai Xue-min 2013, 17 (1):  150-155.  doi: 10.3969/j.issn.2095-4344.2013.01.024 Abstract ( 327 )   PDF (341KB) ( 756 )   Save BACKGROUND: Diabetic nephropathy-caused end-stage renal disease has become the main reason of chronic renal failure. Traditional hemodialysis and kidney transplantation cannot meet clinical needs. Regenerative medicine research based on stem cells brings new hope for treatment of diabetic nephropathy. OBJECTIVE: To comprehensively analyze the mechanism underlying different sources of stem cells for treatment of diabetic nephropathy and the clinical implications. METHODS: Papers addressing stem cells for the treatment of diabetic nephropathy were retrieved by computer in CNKI database and PubMed database from January 2006 to August 2011 with the key words "embryonic stem cells, bone marrow mesenchymal stem cells, induced pluripotent stem cells, diabetic nephropathy" in Chinese and English. Papers published recently or in journals with high impact factor were selected. A total of 26 papers were included for review. RESULTS AND CONCLUSION: Embryonic stem cells, bone marrow mesenchymal stem cells, induced pluripotent stem cells have the potential to differentiate into renal histiocyte. A large numbers of experimental studies have shown that stem cells transplantation has a positive effect on recovery of injured kidney. Stem cells transplantation can provide a novel therapy for diabetic nephropathy. Figures and Tables | References | Related Articles | Metrics

Select

Research progress in reprogramming somatic cells into stem cells Ruan Guang-ping, Wang Jin-xiang, Yang Xiao-yan, Song Qiao-qiao, Yao Xiang, Pang Rong-qing, 2013, 17 (1):  156-160.  doi: 10.3969/j.issn.2095-4344.2013.01.025 Abstract ( 298 )   PDF (335KB) ( 649 )   Save BACKGROUND: The use of cloned human embryos gives rise to ethical issues and allows scientists to find alternative ways to reverse differentiation of cells into multi/pluripotent stem cells, which is known as reprogramming. The new method of reprogramming is the focus of attention. OBJECTIVE: To investigate the current status of reprogramming and review the methods of somatic cell reprogramming into stem cells. METHODS: Using the database of CNKI and Pubmed (1983-01/2006-12) to search the related articles about reprogramming. The retrieval words include reprogramming, method, somatic cell, stem cell, differentiation. Papers related reprogramming published recently or in high-impact journals were initially surveyed and 17 papers were included in the final analysis. RESULTS AND CONCLUSION: The reprogramming of a pluripotent stem cell is the consequence of gradual reconstruction of cell structure, genetic change of chromatin, transcription expression and posttranscriptional control. Remodeling of targeted cells requires a stable status of reprogramming and a final reorientation into specific differentiation. Sufficient evidence demonstrates that cell identification is influenced by in vitro operation. The fate of reprogrammed cells needs further in vivo determination. Figures and Tables | References | Related Articles | Metrics

Select

null Xu Zhi-juan, Ouyang Gui-fang 2013, 17 (1):  161-166.  doi: 10.3969/j.issn.2095-4344.2013.01.026 Abstract ( 364 )   PDF (402KB) ( 649 )   Save null Figures and Tables | References | Related Articles | Metrics

Select

Research and clinical application of mesenchymal stem cells modified by cytokines Wang Kun, Ha Xiao-qin 2013, 17 (1):  167-172.  doi: 10.3969/j.issn.2095-4344.2013.01.027 Abstract ( 253 )   PDF (421KB) ( 583 )   Save BACKGROUND: Cell therapy combined with gene therapy in which mesenchymal stem cells can be modified by different cytokines can capacitate mesenchymal stem cells and enhance their functions. But mesenchymal stem cell function will be restricted in the northwest plateau hypoxia environment. BJECTIVE: To review the research progress in mesenchymal stem cells, and cell function expansion after combined application of cytokines, and search a new means to give full play to cell function in the highaltitude hypoxia environment. METHODS: Articles involving mesenchymal stem cells were searched in CNKI database and PUBMED database through computer, with the keywords “mesenchymal stem cells, hypoxia inducible factor, hepatocyte growth factor”. A total of 71 articles were retrieved. Articles regarding mesenchymal stem cells and their clinical application were selected. The articles published recently in high impact factor journals were selected in the same field. RESULTS AND CONCLUSION: Finally, there are 30 articles selected according to the standard. Through cytokine transfection, mesenchymal stem cells are endowed with new abilities, which enrich the function of mesenchymal stem cells and play an effective role in clinical treatment, in particular in the high altitude hypoxia environment. Figures and Tables | References | Related Articles | Metrics

Select

Stem cell transplantation for the treatment of diabetic foot Wang Guang-yu, Zhu Lü-yun, Ma Li-cheng, Hu Li-ye, Li Xiao-ling, Yang Shao-ling, Shan Wei, 2013, 17 (1):  173-180.  doi: 10.3969/j.issn.2095-4344.2013.01.028 Abstract ( 507 )   PDF (304KB) ( 666 )   Save BACKGROUND: Autologous bone marrow and umbilical blood mononuclear cell transplantation can improve the clinical symptoms caused by ischemic lesions and peripheral nerve injury, and have good effect on diabetic foot. Umbilical cord-derived mesenchymal stem cells have their own advantages when compared with bone marrow stem cells and umbilical cord blood stem cells. OBJECTIVE: To observe the effect of umbilical cord-derived mesenchymal stem cells on diabetic foot ischemic lesions and peripheral nerve injury. METHODS: Thirty-two patients with diabetic foot who received umbilical cord-derived mesenchymal stem cell transplantation from 2010 to 2012 were selected. Umbilical cord-derived mesenchymal stem cells were intramuscularly injected into the bilateral lower extremities with 3 cm distance between two injection sites after dilution. The number of implanted cells was (5.02±1.37) ×108 per leg. The lower extremity ischemia and lower extremity peripheral neuropathy were evaluated at 3 and 6 months after injection. The CMKI database was searched for the literatures in order to analyze the research situation of stem cell transplantation for the treatment of diabetic foot in China. RESULTS AND CONCLUSION: There was no significant difference in ankle/brachial index after treatment. The pain score and the apathetic score were significantly improved at 6 months after transplantation. There were significant differences in angina cruris, skin temperature and transcutaneous oxygen partial pressure after 3 or 6 months of treatment. After treatment for 6 months, there were significant differences in rational symptom score, clinical examination score and vibration perception threshold. There were no significant differences in the conduction velocity of motor/sensory nerves of peroneal nerve and tibial nerve at 3 months after transplantation when compared with those before transplantation (P > 0.05), and there were significant differences at 6 months after transplantation when compared with those before transplantation (P < 0.05). The clinical symptoms and objective indexes of diabetic ischemic lesions and peripheral neuropathy can be improved by umbilical cord- derived mesenchymal stem cells. Literatures analysis has shown that the therapeutic effect in the existing research is satisfactory, and the transplantation method in most researches is consistent with that in this research. Figures and Tables | References | Related Articles | Metrics

Select

Autologous peripheral blood stem cell transplantation for treatment of peripheral nerve injury Jin Wen-hu, Wei Zai-rong, Sun Guang-feng,Tang Xiu-jun, Wang Da-li 2013, 17 (1):  181-185.  doi: 10.3969/j.issn.2095-4344.2013.01.029 Abstract ( 590 )   PDF (337KB) ( 536 )   Save BACKGROUND: Neural stem cells for treatment of peripheral nerve injury have been much reported. But few studies are reported regarding peripheral blood stem cells for treatment of peripheral nerve injury. OBJECTIVE: To investigate the clinical application of autologous peripheral blood stem cells transplantation reinnervating denervated skeletal muscle in the treatment of peripheral nerve injury. METHODS: Six patients received peripheral blood stem cells transplantation for treatment of peripheral nerve injury. At the same time, 10 controls received only anastomosis or nerve transplantation. All patients were intramuscularly administered mouse nerve growth factors one or two treatment courses. In addition, acupuncture, physical therapy, transcutaneous electrical stimulation and functional rehabilitation were also given.  RESULTS AND CONCLUSION: Two groups of patients were followed up over 6 months. The motor nerve and sensory nerve recovered better in patients who received peripheral blood stem cells transplantation than in patients who underwent simple neural anastomosis. These findings suggest that peripheral blood stem cells transplantation can reinnervate the distal denervated skeletal muscle after peripheral nerve injury. Figures and Tables | References | Related Articles | Metrics