Effects of drynaria total flavonoid on osteogenic differentiation of rat dental pulp stem cells via PI3K/Akt pathway

doi:10.3969/j.issn.2095-4344.2013.01.015

Abstract

Abstract:

BACKGROUND: PI3K/Akt pathway may play an important regulative role in osteogenic differentiation of dental pulp stem cells induced by drynaria total flavonoid.
OBJECTIVE: To explore the effects of total flavonoids of drynaria on osteogenic differentiation of rat dental pulp stem cells and investigate the role of PI3K/Akt pathway in the induced differentiation of rat dental pulp stem cells.
METHODS: Rat dental pulp stem cells were harvested by enzymatic digestion, cultured and identified by immunohistochemical staining. The dental pulp stem cells were cultured in media with different concentrations of drynaria total flavonoid (0.01 g/L, 0.05 g/L and 0.1 g/L). Alkaline phosphatase activities and formation of calcified tubercles in cells were determined in each group. Phosphorylated Akt expression in cells was detected by western blot method in each group.
RESULTS AND CONCLUSION: Dental pulp stem cells were positive for CD44 and CD29 expression, but they were negative for CD34. Compared to the blank control group, alkaline phosphatase activities, formation of calcified tubercles and phosphorylated Akt expression were significantly increased in the drynaria total flavonoid group in a dose- and time-dependent manner. These findings suggest that drynaria total flavonoid can promote the osteogenic differentiation of rat dental pulp stem cells, and this effect is likely mediated by PI3K/Akt pathway.

Key words: stem cells, stem cells and traditional Chinese medicine, dental pulp stem cells, drynaria total flavonoid, PI3K/Akt pathway, osteogenic differentiation, stem cell photographs-containing paper

CLC Number:

:R394.2

Huang Xiao-fei, Yuan Su-jian, Yang Cheng. Effects of drynaria total flavonoid on osteogenic differentiation of rat dental pulp stem cells via PI3K/Akt pathway[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(1): 92-97.

Figures/Tables 6

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