Effect of activated autologous platelet-rich plasma on chondrogenic differentiation of rabbit bone marrow-derived mesenchymal stem cells in vitro

doi:10.3969/j.issn.2095-4344.2013.01.001

Abstract

Abstract:

BACKGROUND: Platelet-rich plasma, when activated, could secret multiple growth factors which may promote the proliferation and differentiation of bone marrow-derived mesenchymal stem cells.
OBJECTIVE: To explore the effect of activated autologous platelet-rich plasma on the chondrogenic differentiation of rabbit bone marrow-derived mesenchymal stem cells in vitro.
METHODS: Rabbit bone marrow-derived mesenchymal stem cells were isolated using the whole bone marrow adherence method. Passage 3 bone marrow-derived mesenchymal stem cells were in vitro cultured with 10% activated autologous platelet-rich plasma and 10% fetal bovine serum separately. Chondrogenic differentiation of rabbit bone marrow-derived mesenchymal stem cells in vitro was observed.
RESULTS AND CONCLUSION: Bone marrow-derived mesenchymal stem cells were successfully isolated and exhibited a long-shuttle-shaped appearance. Cells grew faster when passaged. Immunofluorescence staining showed that after induced by activated autologous platelet-rich plasma, bone marrow-derived mesenchymal stem cells exhibited type Ⅱ collagen expression. Quantitative real-time PCR analysis showed that the expression of α1 chain of type Ⅱ collagen and aggrecan mRNA was significantly up regulated in the bone marrow-derived mesenchymal stem cells induced by activated autologous platelet-rich plasma than in the cells induced by fetal bovine serum (P < 0.01). Our findings in this study suggested that activated autologous platelet-rich plasma can promote the chondrogenic differentiation of rabbit bone marrow-derived mesenchymal stem cells in vitro.

Key words: stem cells, bone marrow-derived stem cells, platelet-rich plasma, bone marrow mesenchymal stem cells, chondrocytes, differentiation, type Ⅱ collagen, type Ⅱ collagen α1 chain, aggrecan, rabbits, articular cartilage damage, provincial grants-supported paper, stem cell photographs-containing paper

CLC Number:

R394.2

Wang Shan-zheng, Wang Chen, Rui Yun-feng. Effect of activated autologous platelet-rich plasma on chondrogenic differentiation of rabbit bone marrow-derived mesenchymal stem cells in vitro[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(1): 1-8.

Figures/Tables 7

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