Generation of induced pluripotent stem cells from villus cells after induction by four kinds of genes during prenatal diagnosis

doi:10.3969/j.issn.2095-4344.2013.01.014

Chinese Journal of Tissue Engineering Research ›› 2013, Vol. 17 ›› Issue (1): 86-91.doi: 10.3969/j.issn.2095-4344.2013.01.014

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Generation of induced pluripotent stem cells from villus cells after induction by four kinds of genes during prenatal diagnosis

Luo Yu-mei, Fan Yong, Chen Xin-jie, Yue Lei, Li Qing, Yu Bo-lan, He Wen-zhi, Ma Xiao-yan, Sun Xiao-fang

Key Laboratory for Major Obstetric Diseases of Guangdong Province, Key Laboratory of Reproduction and Hereditary of the Universities in Guangdong Province, the Third Affiliated Hospital of Guangzhou Medical College, Guangzhou 510150, Guangdong Province, China

Received:2012-02-29 Revised:2012-05-28 Online:2013-01-01 Published:2013-01-01
Contact: Sun Xiao-fang, Master, Professor, Master’s supervisor, Key Laboratory for Major Obstetric Diseases of Guangdong Province, Key Laboratory of Reproduction and Hereditary of the Universities in Guangdong Province, the Third Affiliated Hospital of Guangzhou Medical College, Guangzhou 510150, Guangdong Province, China xiaofangsun@hotmail.com
About author:Luo Yu-mei★, Master, Junior Technician, Key Laboratory for Major Obstetric Diseases of Guangdong Province, Key Laboratory of Reproduction and Hereditary of the Universities in Guangdong Province, the Third Affiliated Hospital of Guangzhou Medical College, Guangzhou 510150, Guangdong Province, China mei.2002@163.com
Supported by:
Supported by: the National Natural Science Foundation of China, No. 31171229*; Technology Planning Project of Guangdong Province, No. 2009A030200010*; Guangdong Key Science and Technology Program, No. 2011A080300002

Abstract

Abstract:

BACKGROUND: Due to pluripotent characteristics, induced pluripotent stem cells can be induced to differentiate into the specific cells, including nerve cells and hematopoietic cells.
OBJECTIVE: To establish the induced pluripotent stem cells generated from villus cells during prenatal diagnosis.
METHODS: The prenatal diagnosis villus cells were induced by retroviral vectors encoding hOct4, hSox2, hKlf4 and hc-Myc. The pluripotency, differentiation ability and karyotype of the induced pluripotent stem cells were detected.
RESULTS AND CONCLUSION: The induced pluripotent stem cells maintained the self-renewal ability with high pluripotent marker gene and protein expression and had in vivo and in vitro differentiation potential. The induced pluripotent stem cells could maintain normal karyotype in vitro after long-term culture. The induced pluripotent stem cells obtained from the prenatal diagnosis villus cells provide an ideal source for autologous cell-replacement therapy in the later life of the fetus, and can be used as a perfect model for the research of mechanism underlying prenatally diagnosed diseases.

Key words: stem cells, stem cell culture and differentiation, induced pluripotent stem cells, villus cells, prenatal diagnosis, autologus transplantation, models, National Natural Science Foundation of China, stem cell photographs-containing paper

CLC Number:

R394.2

Luo Yu-mei, Fan Yong, Chen Xin-jie, Yue Lei, Li Qing, Yu Bo-lan, He Wen-zhi, Ma Xiao-yan, Sun Xiao-fang. Generation of induced pluripotent stem cells from villus cells after induction by four kinds of genes during prenatal diagnosis[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(1): 86-91.

Figures/Tables 7

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Generation of induced pluripotent stem cells from villus cells after induction by four kinds of genes during prenatal diagnosis

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