In vitro induced differentiation of bone marrow mesenchymal stem cells into bile duct epithelial-like cells

doi:10.3969/j.issn.2095-4344.2013.01.003

Abstract

Abstract:

BACKGROUND: Extrahepatic bile duct and gallbladder epithelial cells are easily isolated and purified, but bile duct epithelial cells predispose to lose proliferative capacity under in vitro circumstances. Thus, enough amounts of cells used for basic studies are hardly acquired, which limits the progression of bile duct repair. Bone marrow mesenchymal stem cells can be differentiated into hepatocytes, but there have been no reports regarding in vitro induced differentiation of bone marrow mesenchymal stem cells into bile duct epithelial cells.
OBJECTIVE: To discuss the feasibility of inducing bone marrow mesenchymal stem cells into the bile duct epithelial cells in vitro, and to find suitable seed cells for the repair of bile duct injury.
METHODS: Rat bone marrow mesenchymal stem cells were in vitro isolated and purified by whole bone marrow adherent screening in vitro. Passage 3 bone marrow mesenchymal stem cells were cultured with culture medium containing hepatocyte growth factor and epidermal growth factor. The morphological changes of bone marrow mesenchymal stem cells were observed under the inverted microscope and CK19 expression was detected by immunofluorescence assay at different time periods.
RESULTS AND CONCLUSION: Under the induction by hepatocyte growth factor and epidermal growth factor, bone marrow mesenchymal stem cells gradually appeared from shuttle-shaped to polygonal or triangular. Immunofluorescence examination showed that CK19 expression appeared in the cell membrane in the 4th week of induction and it was significantly increased in the 6th week. These findings suggest that bone marrow mesenchymal stem cells can be induced to differentiate into duct epithelial-like cells under the combined induction of hepatocyte growth factor and epidermal growth factor, which provides a new thought for bile duct repair with bone marrow mesenchymal stem cells.
Key Words: stem cells; bone marrow-derived stem cells; bone marrow mesenchymal stem cells; hepatocyte growth factor; epidermal growth factor; bile duct epithelial cells; CK19; stem cell photographs-containing paper

Key words: stem cells, bone marrow-derived stem cells, bone marrow mesenchymal stem cells, hepatocyte growth factor, epidermal growth factor, bile duct epithelial cells, CK19, stem cell photographs-containing paper

CLC Number:

R394.2

Zhang Cheng, Yang Yu-long, Lin Mei-ju, Shi Li-jun, Zhang Hong-wei, Li Jing-yi. In vitro induced differentiation of bone marrow mesenchymal stem cells into bile duct epithelial-like cells[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(1): 17-22.

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