Proliferation and differentiation of neural stem cells from newborn mouse hippocampi in vitro

doi:10.3969/j.issn.2095-4344.2013.01.013

Abstract

Abstract:

BACKGROUND: Neural stem cells transplantation can be used for clinical therapy of central nervous system injury.
OBJECTIVE: To observe the characteristics of proliferation and differentiation of neural stem cells cultured in vitro.
METHODS: Neural stem cells were isolated mechanically from newborn Kunming mouse hippocampus and cultured with serum-free cell culture medium. They were obtained by mechanical dissociation and enzymatic digestion. The neurospheres were identified by immunofluorescent staining of Nestin. The differentiation of neural stem cells was induced by 10% fetal bovine serum and identified by immunohistochemistry of β-Ⅲ-tubulin and glial fibrillary acidic protein. CCK-8 assay was used to ascertain the capacity for neural stem cell proliferation.
RESULTS AND CONCLUSION: The neural stem cells obtained from newborn mouse hippocampus had the capacity for forming the neurospheres which were positive for Nestin. The differentiated cells induced by 10% fetal bovine serum expressed β-Ⅲ-tubulin or glial fibrillary acidic protein. The method of isolation and culture of neural stem cells from newborn mouse hippocampus in vitro was successfully established. Neural stem cells have the capacity for self-renewal and the potential for differentiation.

Key words: stem cells, stem cell culture and differentiation, neural stem cells, cell sphere, cell culture, proliferation, differentiation, stem cell photographs-containing paper

CLC Number:

:R394.2

Hu Ying-ying, Duan Xian-hua, Chen Mei, Wang De-guang. Proliferation and differentiation of neural stem cells from newborn mouse hippocampi in vitro[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(1): 79-85.

Figures/Tables 4

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