Chinese Journal of Tissue Engineering Research ›› 2013, Vol. 17 ›› Issue (1): 74-78.doi: 10.3969/j.issn.2095-4344.2013.01.012

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Subarachnoid space transplantation of superparamagnetic iron oxide-labeled bone marrow mesenchymal stem cells for treatment of spinal cord injury

Zhang Rui-ping1, Li Jian-ding1, Liu Qiang2, Shuang Wei-bing2, Xie Jun3   

  1. 1 Department of Radiology, First Hospital, Shanxi Medical University, Shanxi 030001, Taiyuan Province, China
    2 Department of Orthopedics, First Hospital, Shanxi Medical University, Shanxi 030001, Taiyuan Province, China
    3 Department of Molecular Biology, Shanxi Medical University, Taiyuan 030001, Shanxi Province, China
  • Received:2012-02-19 Revised:2012-04-29 Online:2013-01-01 Published:2013-01-01
  • Contact: Xie Jun, Professor, Department of Molecular Biology，Shanxi Medical University, Taiyuan 030001, Shanxi Province, China xiejunty@yahoo.com
  • About author:Zhang Rui-ping☆, M.D., Associate professor, Department of Radiology, First Hospital, Shanxi Medical University , Shanxi 030001, Taiyuan Province, China zrp_7142@163.com
  • Supported by:

    Supported by: Shanxi Province Youth Science and Technology Research Foundation, No. 2010021038-2*, Science and Technology Foundation of Shanxi Provincial Health Department, No. 2011017

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Abstract:

BACKGROUND: Transplantation of stem cells can rebuild the structure and function of injured central nervous system, and has attracted wide attention in recent years.
OBJECTIVE: To explore the effects of bone marrow mesenchymal stem cells labeled by superparamagnetic iron oxide on the recovery of neurological function in rabbits with spinal cord injury.
METHODS: Bone marrow mesenchymal stem cells were isolated from rabbits, and cultivated in vitro using the density gradient centrifugation and the adherence in vitro separation. Passage 3 bone marrow mesenchymal stem cells were labeled with superparamagnetic iron oxide at 24 hours before use. Rabbit spinal cord injury models were made and the micro tubes were inserted into subarachnoid space. Rabbits were randomly divided into three groups．Rabbits from the group A were injected with superparamagnetic iron oxide labeled bone marrow mesenchymal stem cells via subarachnoid spaces. Rabbits from the group B received transplan tation of unlabeled bone marrow mesenchymal stem cells. Rabbits from the group C were injected with PBS and served as controls. At 1, 7, 14, 21, 28 and 35 days after cell transplantation, the recovery of neurological function was calculated by BBB scoring in all groups and the pathological tissue slices of spinal cord injury was examined.
RESULTS AND CONCLUSION: The BBB scores in the groups A and V were significantly higher than those in the group C (P < 0.05), but there was no statistical difference between groups A and B (P > 0.05). At 1, 7, 14, 21, 28 and 35 days following cell transplantation, Prussian blue staining of tissue sections showed cells containing blue iron particles in the area of spinal cord injury. Bone marrow mesenchymal stem cells transplanted via subarachnoid space could migrate to the area of spinal cord injury, which thereby improved neurological function.

Key words: stem cells, stem cell transplantation, superparamagnetic iron oxide, bone marrow mesenchymal stem cells, spinal cord injury, cell transplantation, subarachnoid space, provincial grants-supported paper, stem cell photographs-containing paper

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