Effects of eupolyphaga-containing serum on proteomics changes in bone marrow mesenchymal stem cells

doi:10.3969/j.issn.2095-4344.2013.01.016

Abstract

Abstract:

BACKGROUND: Avascular necrosis of the femoral head has been much studied. However, the precise pathological mechanism underlying avascular necrosis of the femoral head remains unclear.
OBJECTIVE: To investigate the effects of eupolyphaga-containing serum on the target or related proteins inhibiting adipogenic differentiation and promoting osteogenic differentiation of bone marrow mesenchymal stem cells.
METHODS: Passage 3 bone marrow mesenchymal stem cells were cultured with DMEM containing blank serum (blank control group), dexamethasone (hormone group) and eupolyphaga-containing serum (eupolyphaga group). After 6 days of culture, isobaric tag for relative and absolute quantitation technique was used to quantify proteins from three groups. The proteins were identified and relatively quantitatively analyzed by mass spectrometry.
RESULTS AND CONCLUSION: Relative to the hormone group, there were 15 proteins significantly up regulated and 12 proteins significantly down regulated in the blank control group, and there were 16 proteins significantly up regulated and 14 proteins significantly down regulated in the eupolyphaga group. Hspa1L1 and PrxⅤ may be the targets of anti-apoptosis in eupolyphaga. The expression of Serpinh1 and ADP/ATP translocase 1 may be the targets of promoting osteogenic differentiation in eupolyphaga.

Key words: stem cells, bone marrow-derived stem cells, stem cells and traditional Chinese medicine, eupolyphaga, differentiation, drug-containing serum, two-dimensional liquid chromatography, differential proteomics, cell apoptosis, provincial grants-supported paper

CLC Number:

R394.2

Zhong Wei-hong, Qi Zhen-xi2. Effects of eupolyphaga-containing serum on proteomics changes in bone marrow mesenchymal stem cells[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(1): 98-105.

Figures/Tables 4

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