Biocompatibility of an acellular brain tissue scaffold with bone marrow mesenchymal stem cells

doi:10.3969/j.issn.2095-4344.2013.14.004

Abstract

Abstract:

BACKGROUND: Use of scaffolds loaded with bone marrow mesenchymal stem cells for treatment of central nervous system injury is still in the stage of laboratory study.
OBJECTIVE: To evaluate the biocompatibility of acellular brain tissue scaffold with rat bone marrow mesenchymal stem cells in vitro and to testify whether the material could be used as a carrier for central nervous system tissue engineering.
METHODS: Bone marrow mesenchymal stem cells were isolated and purified by whole bone marrow method, and the acellular brain tissue scaffold was made by both physical and chemical extraction methods. Bone marrow mesenchymal stem cells transfected by green fluorescent protein were cultured and compounded onto acellular brain tissue scaffold in vitro. The microstructure of the materials and the morphological changes of bone marrow mesenchymal stem cells compounded onto the acellular brain tissue scaffold were observed under inverted phase microscope, scanning electron microscope, and confocal laser scanning microscope．
RESULTS AND CONCLUSION: The acellular brain tissue scaffold had the structure of three-dimensional network. The cells grew in an adherent way on the acellular brain tissue scaffold and had normal shape in vitro. The rat acellular brain tissue scaffold has good biocompatibility with bone marrow mesenchymal stem cells and might be used as a biological material for central nervous system tissue engineering.

Key words: stem cells, bone marrow-derived stem cells, bone marrow mesenchymal stem cells, acellular, scaffold, three-dimensional, network, biocompatibility, tissue engineering, brain tissue, green fluorescent protein, bioactivity, National Natural Science Foundation of China, stem cell photographs-containing paper

CLC Number:

null

Ding Ming-xiang, Lin Shao-hua, Li Liang-ming, Hou Bo, Liang Chao-feng, Gong Jin, . Biocompatibility of an acellular brain tissue scaffold with bone marrow mesenchymal stem cells[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(14): 2495-2500.

Figures/Tables 6

References

[1] Gilbert TW, Sellaro TL, Badylak SF. Decellularization of tissues and organs. Biomaterials 2006;27(19):3675-3683.

[2] Rochkind S, Shahar A, Fliss D, et al. Development of a tissue-engineered composite implant for treating traumatic paraplegia in rats. Eur Spine J.2006;15(2):234-245.

[3] Li Q, Geng YJ, Lu L, et al. Platelet-rich fibrin-induced bone marrow mesenchymal stem cell differentiation into osteoblast-like cells and neural cells. Neural Regen Res. 2011;6(31):2419-2423.

[4] Mareschi K, Novara M, Rustichelli D, et al. Neural differentiation of human mesenchymal stem cells: Evidence for expression of neural markers and eag K+ channel types. Exp Hematol. 2006;34(11):1563-1572.

[5] Wang FW, Jia DY, Du ZH, et al. Roles of activated astrocytes in bone marrow stromal cell proliferation and differentiation. Neuroscience.2009;160(2):319-329.

[6] Yin YQ, Chen B, Ke JL, et al. Xuefuzhuyu injection induces neuronal differentiation of rat bone morrow mesenchymal stem cells. Neural Regen Res. 2011;6(3):177-182.

[7] Chen J, Li Y, Wang L, et al. Therapeutic benefit of intravenous administration of bone marrow stromal cells after cerebral ischemia in rats.Stroke.2001;32(4): 1005-1011.

[8] Qu C, Mahmood A, Lu D, et al. Treatment of traumatic brain injury in mice with marrow stromal cells. Brain Res.2008; 1208:234-239.

[9] Wang D, Zhang JJ. Electrophysiological functional recovery in a rat model of spinal cord hemisection injury following bone marrow-derived mesenchymal stem cell transplantation under hypothermia. Neural Regen Res. 2012;7(10): 749-755.

[10] Parr AM, Tator CH, Keating A. Bone marrow-derived mesenchymal stromal cells for the repair of central nervous system injury. Bone Marrow Transplant.2007; 40(7):609-619.

[11] Badylak SF. Regenerative medicine and developmental biology:the role of the extracellular matrix. Anal Rec B New Anat.2005;287(1):36-41.

[12] Badylak SF. The extracellular matrix as a biologic scaffold material. Biomaterials.2007;28(25):3587-3593.

[13] Walsh S, Biernaskie J, Kemp SW, et al. Supplementation of acellular nerve grafts with skin derived precursor cells promotes peripheral nerve regeneration. Neuroscience.2009; 164(3):1097-1107.

[14] Luo JC, Chen W, Chen XH, et al. A multi-step method for preparation of porcine small intestinal submucosa (SIS). Biomaterials.2011;32(3):706-713.

[15] Ott HC, Matthiesen TC, Goh SK,et al. Perfusion-decellularized matrix using nature’s platform to engineer a bioartificial heart.Nat Med.2008;14(2):213-221.

[16] Yang B, Zhang YF, Zhou LH, et al. Development of a porcine bladder acellular matrix with well-preserved extracellular bioactive factors for tissue engineering. Tissue Eng Part C Methods. 2010;16(5):1201-1211.

[17] Ribatti D, Conconi MT, Nico B, et al. Angiogenic response induced by acellular brain scaffolds grafted onto the chick embryo chorioallantoic membrane. Brain Res.2003;989(1): 9-15.

[18] Gong J, Wang H, Shi DJ, et al. Zhonghua Shengwu Yixue Gongcheng Zazhi. 2010;16(6):552-556.龚瑾,王辉,石德金,等.脱细胞脑组织支架初步制备及形态学观察[J].中华生物医学工程杂志,2010,16(6):552-556.

[19] Sondell M, Lundborg G, Kanje M. Regeneration of the rat sciatic nerve into allografts made acellular through chemical extraction.Brain Res.1998;795(1-2):44-45.

[20] Mekhail M, Wong KK, Padavan DT, et al. Genipin-Cross- linked electrospun collagen fibers.J Biomater Sci Polym Ed. 2010;96(1):204-211.

[21] Lien SM, Li WT, Huang TZ. Genipin-cross linked gelatin scaffolds for articular cartilage tissue engineering with a novel crosslinking method. Materials Science and Engineering C. 2008;28(1):36-43.

[22] Engler AJ, Sen SH,Sweeney HL, et al. Matrix Elasticity Directs Stem Cell Lineage Speci?cation. Cell. 2006;126(4): 677-689.