Abstract:

BACKGROUND: Cdh1 has been shown to express in rat hippocampus and cortex in a large number. Moreover, in vitro test demonstrated that Cdh1 expression was higher in neurons than in neural stem cells, which possibly associated with the differentiation of neural stem cells into neurons. However, the effects of anaphase promoting complex Cdh1 on ischemic neuronal damage remain unclear.
OBJECTIVE: To investigate the expression of Cdh1 and its downstream substrate in primary cultured neurons with oxygen-glucose deprivation.
METHODS: Primary neurons from cortex of postnatal 24-hour rat pups were cultured in vitro, and identified by immunofluorescence staining. The oxygen- and glucose-deprived models were established by three gas incubator filled with nitrogen in sugar-free Earle’s solution. After 1 hour of hypoxia, reoxygenation was conducted. Real-time fluorescent quantitative PCR was used to detect the mRNA expression of Cdh1 and its downstream substrates Skp2, Cyclin B1 before hypoxia, 6 hours, 1, 3, 7 days after oxygen glucose deprivation.
RESULTS AND CONCLUSION: After oxygen glucose deprivation, the expression of Cdh1 and Cyclin B1 in primary neurons was increased (P < 0.05), while Skp2 expression was decreased (P < 0.05). Above data indicated that Cdh1 expression in neurons increased after oxygen-glucose deprivation. It may degrade Skp2 and participate in hypoxic neuronal apoptosis by ubiquitination.

Key words: Stem Cells, Anoxia, Neurons, Cyclins