Tissue engineered artificial nerves repair sciatic nerve defect in rats: an evaluation using horseradish peroxidase retrograde tracer technique

doi:10.3969/j.issn.2095-4344.2014.29.012

Abstract

Abstract:

BACKGROUND: We have previously prepared acellular nerve graft and implanted bone marrow mesenchymal stem cells into the graft, to successfully construct tissue engineered artificial nerves.

OBJECTIVE: Horseradish peroxidase nerve retrograde tracer technique was used to evaluate protective effects on sensory neurons following sciatic nerve defect bridging with tissue engineered artificial nerves constructed by acellular nerve graft and bone marrow mesenchymal stem cells in rats.

METHODS: Adult, clean, healthy, male Sprague-Dawley rats were randomly assigned to three group: (1) Experimental group: Rat sciatic nerve detect was bridged by acellular nerve graft combined with bone marrow mesenchymal stem cells; (2) Blank control group: Rat sciatic nerve defect was bridged by acellular nerve graft; (3) Autologous nerve control group: Rat sciatic nerve defect was bridged by autologous nerve transplantation. Regeneration of sensory neurons in the spinal dorsal root ganglia was assessed using horseradish peroxidase nerve retrograde tracer technique at 12 weeks following surgery.

RESULTS AND CONCLUSION: Sensory neuron regeneration in the spinal dorsal root ganglia at 12 weeks following surgery was better in the experimental group compared with blank control group. No significant difference was detected between experimental group and autologous nerve control group. S-100 immunohistochemical staining in plantar skin showed brown positive reaction in each group. These findings indicate that tissue engineered artificial nerves constructed by acellular nerve graft and bone marrow mesenchymal stem cells have protective effects on sensory neurons in the spinal dorsal root ganglia, and can promote the recovery of sensory function and repair sciatic nerve defect in rats.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: stem cells, mesenchymal stem cells, sciatic nerve, ganglia, neuronal tract-trancer

CLC Number:

R318

Zhang Cai-shun, Lv Gang. Tissue engineered artificial nerves repair sciatic nerve defect in rats: an evaluation using horseradish peroxidase retrograde tracer technique[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(29): 4658-4662.

Figures/Tables 1

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