Apoptosis of human embryonic cerebral cortex neurons transfected with human telomerase reverse transcriptase

doi:10.3969/j.issn.2095-4344.2014.29.011

Abstract

Abstract:

BACKGROUND: Human telomerase reverse transcriptase recombinant adenovirus transfection could promote the survival and inhibit the apoptosis of primary cultured human embryonic cerebral cortex neurons.

OBJECTIVE: To observe the effects of human telomerase reverse transcriptase on the apoptosis of human embryonic cerebral cortex neurons induced by amyloid β-protein invention.

METHODS: Primary cultured cerebral cortex neurons of human embryo were divided into three groups, namely control group, amyloid β-protein (25-35) group, and human telomerase reverse transcriptase group. Except the control group, the cells were cultured for 144 hours and intervened with 5 μmol/L amyloid β-protein (25-35) for 24 hours. Furthermore, the cells in human telomerase reverse transcriptase group were transfected with human telomerase reverse transcriptase group at 72 hours after intervention of 5 μmol/L amyloid β-protein (25-35).

RESULTS AND CONCLUSION: After human embryonic cerebral cortex neurons were cultured for 7 days, the apoptotic cells were found in three groups. The apoptosis of neurons was obviously increased after intervention of amyloid β-protein (25-35). Human telomerase reverse transcriptase transfection could prevent the apoptosis of human embryonic cerebral cortex neurons induced by amyloid β-protein (25-35).

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: cerebral cortex, apoptosis, telomerase, telomerase-binding proteins, transfection

CLC Number:

R318

Wu Ling-zhi, Li Shui-bin, Cheng Gang-wei, Wang Hua-qiao, Kong Ling-ping. Apoptosis of human embryonic cerebral cortex neurons transfected with human telomerase reverse transcriptase[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(29): 4653-4657.

Figures/Tables 2

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