Effects of different cervical artificial disc coatings on adhesion and differentiation ability of rat bone marrow mesenchymal stem cells

doi:10.3969/j.issn.2095-4344.2014.12.022

Abstract

Abstract:

BACKGROUND: Titanium artificial cervical disc has good biocompatibility, but titanium alloy is shown to have poor biological activity, low bonding strength, easy release of metal ions under physiological conditions.
OBJECTIVE: To observe the effects of different coating for the titanium plate of domestic cervical artificial disc on adhesion and differentiation ability of rat bone marrow mesenchymal stem cells.
METHODS: Passage 3 bone marrow mesenchymal stem cells from Wistar rats were seeded into 24-well titanium plates with hydroxyapatite coating, titanium powder+hydroxyapatite coating and bare titanium plate. Cell culture was terminated after 24 and 48 hours, and the cell growth was observed under scanning electron microscope. After 24 hours of inoculation, osteogenic inducer was added; then, cell supernatant was collected at 3, 5, 7 days after cell lysis and centrifugation to detect the activity of alkaline phosphatase.
RESULTS AND CONCLUSION: After composite culture with titanium plates with hydroxyapatite coating or titanium powder+hydroxyapatite coating, cultured cells were in polygonal shape, and pseudopodia were extended into the micropores that were adhered closely to the material surface. Cells cultured with bare titanium plates had poor differentiation and adhesion rate. With time, the expression of alkaline phosphatase was increased in each group, especially in the groups of titanium plates with hydroxyapatite coating and titanium powder+hydroxyapatite coating (P < 0.05). These findings indicate that titanium plates with hydroxyapatite coating or titanium powder+hydroxyapatite coating can promote adhesion and differentiation of rat bone marrow mesenchymal stem cells.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: biocompatible materials, intervertebral disk, bioprosthesis, stem cells

CLC Number:

R318

Li Jun-hui, Yu Xing, Mu Xiao-hong, Xu Lin, Wang Song, Lu Jun-zhe . Effects of different cervical artificial disc coatings on adhesion and differentiation ability of rat bone marrow mesenchymal stem cells[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(12): 1938-1943.

Figures/Tables 3

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