In vitro biocompatibility of expanded polytetrafluoroethylene scaffold and human adipose-derived stem cells

doi:10.3969/j.issn.2095-4344.2014.12.021

Abstract

Abstract:

BACKGROUND: Expanded polytetrafluoroethylene is a kind of porous polymer materials which is commonly used as clinical implants, and it has good biocompatibility, and is not easy to deformation or metamorphism. There is no existence of inflammation absorption reaction, and it allows the cell migration and tissue ingrowth.
OBJECTIVE: To study the biocompatibility of expanded polytetrafluoroethylene scaffold and human adipose-derived stem cells.
METHODS: The passage 4 human adipose-derived stem cells were co-cultured with expanded polytetrafluoroethylene scaffold in vitro. The morphology and function of cells adhered to the scaffold were observed by inverted phase contrast microscope, and cell adhesive rates and proliferation rates were also calculated by MTT assay.
RESULTS AND CONCLUSION: The inoculated cells were round and bright, distributed on the surface of scaffolds uniformly, with good cell viability. After 3 hours a large number of adherent cells were observed from the micrograph; after 24 hours there were a small amount of short-spindle adipose-derived stem cells. After cultured for 3 days, the short fusiform or polygon cells could be seen clearly. After cultured for 7 days, the number of cells increased significantly, few cells fell off from the scaffold, and cell adhesion rate was up to an average of 95.7%. Meanwhile, the cells revealed normal splitting proliferation rate. These findings indicate that human adipose-derived stem cells are able to attach, grow and proliferate well on the scaffold. Expanded polytetrafluoroethylene reveals excellent cellular compatibility and can be used as a vehicle for adipose tissue engineering.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: biocompatible materials, polytetrafluoroethylene, stem cells, materials testing

CLC Number:

R318

Yang Liu, Jiang Nan, Xu Yang-yang, Zhu Meng-lin, Cao Jing . In vitro biocompatibility of expanded polytetrafluoroethylene scaffold and human adipose-derived stem cells [J]. Chinese Journal of Tissue Engineering Research, 2014, 18(12): 1932-1937.

Figures/Tables 3

References

[1] De Ugarte DA,Morizono K,Elbarbary A,et al.Comparison of multi-lineage cells from human adipose tissue and bone marrow.Cells Tissues Organs.2003;174(3):101-109.

[2] Mizuno H.Adipose-derived stem cells for tissue repair and regeneration: ten years of research and a literature review.J Nippon Med Sch.2009;76(2):56-66.

[3] 费剑锋,宋红权,孙洋,等.不同支架材料及接种培养方法对前脂肪细胞生长情况的影响[J].中华医学美学美容杂志, 2010,16(4):252-257.

[4] 张云松,高建华,鲁峰,等.Ⅰ型胶原支架材料与人脂肪干细胞体外生物相容性研究[J].南方医科大学学报,2007;27(2):223-225.

[5] Ham J,Miller PJ.Expanded ploytetra fluoroethylene implants in rhinoplasty, Literature review, operative techniques and outcome.Facial Plast Surg.2003;19(4):331.

[6] Kridel RWH,Kraus WM.Grafts and implants in revision hinoplasty.Facial Plast Surg Clin Norty Am.1995;3(4):473.

[7] 张召,熊雯,郑晋东,等.膨体聚四氟乙烯假体隆鼻术并发症的分析及防治[J].中国美容医学,2010,19(5);678-679.

[8] Mole B.The use of Gore-Tex implants in aesthetic surgery of the face. Plast Reconstr Surg.1992;2:200.

[9] Conrad K,Chapnik JS,Reifen E.E-PTEE (Gore-Tex) suspension cervical facial rhytidectomy.Arch Otolaryngol Head Neck Surg.1993;119:684.

[10] Zuk PA,Zhu M,Mizuno H,et al.Multilineage cells from humanadiposetissue:implicationsforcell-based therapies. Tissue Eng.2001;7:211-228.

[11] Zuk PA,ZhuM,ASHJIANP,et al.Human adipose tissue is a source of multipotent stem cells.Mol Biol Cell.2002; 13(12): 4279-4259.

[12] Yoshimura K,Shigeura T,Mastsumoto D,et al.Characterization of freshly isolated and cultured cells derived from the fatty and fluid portions of liposuction aspirates.Cell Physiol.2006;208 (1):64-76.

[13] Mitchell JB,McIntosh K,Zvonic S,et al.Immunophenotype ofhuman adipose-derived cells: Temporal changes in stromal-associatedand stem cell-associated markers.Stem Cells.2006;24:376-385.

[14] Kasten P,Luginbuhl K,van Gnensven M,et al.Comparison of human bone marrow stem cells seeded on calcium-deficient hydroxyapatite , beta-tricalcium phosphate and dematerialized bone matix.Biomaterials.2003;24:2593-2603.

[15] 李德保,田甜,章庆国.膨体聚四氟乙烯(ePTEE)内支撑组织工程软骨的构建实验研究[J].中国美容医学,2009,18(6):823-826.

[16] 王贺宾,刘清波.自体耳软骨与膨体聚四氟乙烯在鼻整形中的应用[J].中外健康文摘,2013,10(4):122-123.

[17] 李春光,陈和忠,李志刚,等.膨体聚四氟乙烯补片在胸壁重建中的应用[J].中国胸心血管外科临床杂志,2012,19(3):248-250.

[18] 袁义,蔡开灿,王振康,等.超微孔膨体聚四氟乙烯人工瓣膜行绵羊二尖瓣置换的血流动力学[J].实用医学杂志,2012,28(14):2318-2320.

[19] 田田,李英春,周斌,等.膨体聚四氟乙烯覆盖创面的实验研究[J].中华烧伤杂志,2009,25(2):133-136.

[20] Neel HB.Implants of Gore-Tex: comparisons with Teflon-coated polytetrafluroethlene carbon and porous polyethylene implants.Arch Otolaryngol.1983;109:427-433.

[21] Taha MF,Hedayati V.Isolation,identification and multipotential differentiation of mouse adipose tissue-derived stem cells. Tissue Cell.2010;42(4):211-216.

[22] Grothos S,Franklin DM,Leddy HA,et al.Surface protein characterization of human adipose tissue-derived stromal cells.J Cell Physiol.2001;189(1):54-63.

[23] Bunnell BA,Flaat M,Gagliardi C,et al.Adipose-derived stem cells: isolation, expansion and differentiation. Methods.2008; 45(2):115-120.