Delivery of DMAPA-Amp wrapped green fluorescent protein by modified carotid injection

doi:10.3969/j.issn.2095-4344.2014.12.020

Abstract

Abstract:

BACKGROUND: Hyperbranched cationic amylopectin is a kind of nonviral gene vectors with low toxicity and good transfection efficiency. However, searching for more efficient methods to delivery it into the body and making the genes expressed are being explored.
OBJECTIVE: To study the expression of DMAPA-Amp wrapped green fluorescent protein (GFP) transferred by modified carotid injection into cerebral ischemic area.
METHODS: Male Sprague-Dawley rats subjected to middle cerebral artery infarction were randomly divided into two groups after 24 hours: experimental group (injected with GFP entrapped DMAPA-Amp via the internal carotid artery) and control group (injected with GFP entrapped DMAPA-Amp via the tail vein). These rats were put to death and their brain tissue was removed after 7 days. The expression of GFP was detected by quantitative PCR and western blot assay, and immunofluorescence staining was performed to detect the expression of GFP located near cerebrovascular endothelial cells by frozen section.
RESULTS AND CONCLUSION: Compared with the control group, the expression of GFP was much higher in the experimental group detected by quantitative PCR and western blot (P < 0.05). Additionally, the expression of GFP located near cerebrovascular endothelial cells by frozen section was also higher than that in the control group. Modified carotid injection could significantly promote the expression of hyperbranched cationic amylopectin derivates and GFP in the brain tissue of Sprague-Dawley rats undergoing middle cerebral artery infarction compared with tail vein injection, which indicates DMAPA-Amp and modified carotid injection may cast new lights on the therapy for angiogenesis of ischemic stroke.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: biocompatible materials, carotid arteries, injections, intra-arterial, green fluorescent proteins, transfection

CLC Number:

R318

Wang Jue, Wang Jing-nan, Deng Yu-bin, Yang Li-qun. Delivery of DMAPA-Amp wrapped green fluorescent protein by modified carotid injection[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(12): 1926-1931.

Figures/Tables 2

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