In vitro biocompatibility of polyhydroxybutyrate-co-volerate membrane with human bone marrow mesenchymal stem cells

doi:10.3969/j.issn.2095-4344.2014.12.019

Abstract

Abstract:

BACKGROUND: Polyhydroxybutyrate-co-volerate (PHBV) is a noticeable tissue engineering material of polyhydroxyalkanoates family. It has the properties of low immune rejection response and good biocompatibility, and its degradation products are non-toxic.
OBJECTIVE: To investigate the biocompatibility of PHBV membrane material and human bone marrow mesenchymal stem cells in vitro.
METHODS: Human bone marrow mesenchymal stem cells at passage 3 were seeded upon PHBV membrane as experimental group and upon conventional culture plates as control group. Then we calculated the adherent cell number of two groups at 1, 2 and 4 hours and got the cell adherent rate. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide assay was used at days 2, 4, 6, 8 to observe the cell proliferation of two groups. Fluorimetric method with the fluorescent dye Hoechst 33258 was used to detect the DNA content of cells at days 3, 6, 9 and 12 in both groups. After cells were seeded upon PHBV membrane for 5 days, the cell growth upon the material was examined under a scanning electron microscope.
RESULTS AND CONCLUSION: When the cells were cultured for 1 hour, the adherent rate in the experimental group was lower than that in the control group; but there were no significant differences between two groups at the other two periods. No difference was found in the cell proliferation and the DNA content between the two groups. Human bone marrow mesenchymal stem cells seeded upon PHBV membrane for 5 days grew well with spindle morphology and the intercellular connections were tight and more extracellular matrices were observed by scanning electron microscopy. Taken together, PHBV membrane material shows a good biocompatibility with human bone marrow mesenchymal stem cells.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: biocompatible materials, stem cells, 3-hydroxybutyric acid, histocompatibility

CLC Number:

R318

Zhang Tao, He Zhi-xu, Ye Chuan, Liu Jie-lin, Ma Min-xian, Sun Bo, Wang Mei, Yang Hua. In vitro biocompatibility of polyhydroxybutyrate-co-volerate membrane with human bone marrow mesenchymal stem cells[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(12): 1920-1925.

Figures/Tables 3

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