Chinese Journal of Tissue Engineering Research ›› 2020, Vol. 24 ›› Issue (1): 130-135.doi: 10.3969/j.issn.2095-4344.1845
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Xu Huijun, Shi Dongmei, Zhang Mi, Wu Saixuan, Dong Ming, Lu Ying, Niu Weidong
Received:
2019-02-25
Revised:
2019-03-05
Accepted:
2019-04-19
Online:
2020-01-08
Published:
2019-12-13
Contact:
Niu Weidong, MD, Professor, Doctoral supervisor, Department of Endodontics, School of Stomatology, Dalian Medical University, Dalian 116044, Liaoning Province, China
About author:
Xu Huijun, Master candidate, Physician, Department of Endodontics, School of Stomatology, Dalian Medical University, Dalian 116044, Liaoning Province, China
Shi Dongmei, Master candidate, Physician, Department of Endodontics, School of Stomatology, Dalian Medical University, Dalian 116044, Liaoning Province, China
Xu Huijun and Shi Dongmei contributed equally to this work
Supported by:
CLC Number:
Xu Huijun, Shi Dongmei, Zhang Mi, Wu Saixuan, Dong Ming, Lu Ying, Niu Weidong. Effect of sex combing protein 1 on proliferation and differentiation of osteoblasts in inflammatory microenvironment#br#[J]. Chinese Journal of Tissue Engineering Research, 2020, 24(1): 130-135.
2.1 CCK-8筛取脂多糖最佳实验质量浓度及实验时间 2.1.1 筛取最佳实验质量浓度 分别加入0,1,10,20,50 mg/L脂多糖刺激MC3T3-E1细胞24 h,各实验组分别与0 mg/L脂多糖组比较,差异有显著性意义(P < 0.05),当质量浓度为1 mg/L时细胞增殖活性少量增加,说明低质量浓度脂多糖刺激可能对成骨细胞增殖起促进作用,而当质量浓度为50 mg/L时细胞增殖活性降低最明显,但此时对细胞杀伤力较大,为确保后续实验顺利进行,最终确定以 20 mg/L为最佳实验质量浓度,见图1A。 2.1.2 筛取最佳作用时间 以0,20 mg/L脂多糖分别刺激0,24,48 h,结果显示实验组细胞的增殖活性呈降低趋势,差异有显著性意义(P < 0.05),且在24 h抑制明显,因此确定最佳实验时间24 h,见图1B。"
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