Chinese Journal of Tissue Engineering Research ›› 2021, Vol. 25 ›› Issue (5): 679-684.doi: 10.3969/j.issn.2095-4344.2998
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Zhang Mi, Wu Saixuan, Dong Ming, Lu Ying, Niu Weidong
Received:
2020-02-25
Revised:
2020-02-29
Accepted:
2020-04-03
Online:
2021-02-18
Published:
2020-11-28
Contact:
Niu Weidong, MD, Professor, Doctoral supervisor, School of Stomatology, Dalian Medical University, Dalian 116041, Liaoning Province, China
About author:
Zhang Mi, Master candidate, Physician, School of Stomatology, Dalian Medical University, Dalian 116041, Liaoning Province, China
Supported by:
CLC Number:
Zhang Mi, Wu Saixuan, Dong Ming, Lu Ying, Niu Weidong. Expression of interleukin-24 in a mouse model of periapical periodontitis[J]. Chinese Journal of Tissue Engineering Research, 2021, 25(5): 679-684.
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2.1 实验动物数量分析 实验选用6周龄C57BL/6雌性小鼠25只,实验过程中无脱失,全部纳入结果分析。 2.2 苏木精-伊红染色结果 通过苏木精-伊红染色观察根尖周部位的组织学变化,以验证诱发性根尖周炎模型是否构建成功,见图1。0周组正常根尖周范围未见明显炎症反应;1 周组:即开髓后暴露7 d,在根尖区域组织中可见炎性细胞小范围分布,牙周膜稍见增宽,这一时期相当于处在炎症发展早期;2周组:即开髓后暴露14 d,炎性细胞分布范围与炎症早期相比进一步扩大,牙槽骨边缘开始出现局部破坏,这一时期相当于处在炎症发展急性期;3周组:即开髓后暴露21 d,炎细胞分布范围进一步扩大,种类多为淋巴细胞和中性粒细胞,根尖骨破坏进一步加重,炎症进入进展期;4周组:即开髓后暴露28 d,炎性细胞浸润及骨破坏仍明显,局部可见纤维结缔组织形成,表明炎症逐渐进入慢性期。这表明小鼠诱发性慢性根尖周炎模型建立成功。"
2.3 RT-PCR结果 通过RT-PCR检测白细胞介素24在小鼠根尖周部位mRNA表达情况,见图2。其中,各实验组根尖区组织中白细胞介素24的mRNA表达均较0周组高(P < 0.05),且也在2 周达到峰值,3周及4周组中白细胞介素24的mRNA表达趋势下降,各组之间比较差异均有显著性意义(P < 0.05)。 2.4 IHC染色结果 通过IHC染色检测白细胞介素24在小鼠根尖周部位中的表达情况,见图3。以根尖周区域黄褐色颗粒染色为阳性细胞,白细胞介素24阳性细胞在各时间点病灶区中均呈阳性表达。0周组正常根尖周围组织中几乎无白细胞介素24表达;各实验组根尖周区域白细胞介素24表达量较0周组明显增高(P < 0.05),且在2周组呈现表达高峰;3周组、4周组表达量较2周组有所降低。3周组与4周组两者之间差异无显著性意义,其他各组之间两两比较差异均有显著性意义(P < 0.05,见图4)。 "
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