Chinese Journal of Tissue Engineering Research ›› 2019, Vol. 23 ›› Issue (6): 821-826.doi: 10.3969/j.issn.2095-4344.1578

    Next Articles

Construction of polycaprolactone-tricalcium phosphate with different mixture ratios using three-dimensional printing technology and its osteoinductivity in vitro

Yuan Bo, Wang Zhiwei, Tang Yifan, Zhou Shengyuan, Chen Xiongsheng, Jia Lianshun   

  1. Spine Center, Department of Orthopedics, Shanghai Changzheng Hospital, Second Military Medical University, Shanghai 200003, China
  • Received:2018-10-19 Online:2019-02-28 Published:2019-02-28
  • Contact: Chen Xiongsheng, MD, PhD, Chief physician, Professor, Doctoral supervisor, Spine Center, Department of Orthopedics, Shanghai Changzheng Hospital, Second Military Medical University, Shanghai 200003, China
  • About author:Yuan Bo, Doctorate candidate, Spine Center, Department of Orthopedics, Shanghai Changzheng Hospital, Second Military Medical University, Shanghai 200003, China
  • Supported by:

    the Major Project of the Science and Technology Commission of Shanghai Municipality, No. 15JC1491003 (to CXS)

PDF

384

Abstract:

BACKGROUND: The choice and mixture ratio of composite materials for bone tissue engineering scaffolds have a great influence on the osteoinductivity of scaffolds.

OBJECTIVE: To construct the polycaprolactone-tricalcium phosphate composite scaffolds with different mixture ratios using 3D printing technology, and to test their physiochemical characteristics and osteoinductivity.
METHODS: Polycaprolactone and tricalcium phosphate were mixed in different ratios of 10:0, 9:1, 8:2, and 7:3, and the polycaprolactone and polycaprolactone-tricalcium phosphate scaffolds were constructed by the 3D printing technology. The scaffolds were named as PCL scaffold, PCL-10% TCP scaffold, PCL-20% TCP scaffold, PCL-30% TCP scaffold. The microstructure and mechanical properties of the scaffolds were characterized by scanning electron microscopy and fatigue testing machine. The proliferation and alkaline phosphatase activity of rabbit bone marrow mesenchymal stem cells seeded on different scaffolds were detected at 1, 4, 7 days of culture and at 1, 7 and 14 days of culture, respectively, by in vitro experiments. The content of calcium in the supernatant of the scaffolds was detected at 7, 14, and 21 days of culture
RESULTS AND CONCLUSION: The PCL-30%TCP scaffold could not be constructed by this method. Compared with the PCL scaffold, the compressive strength and elastic modulus of the PCL-10% TCP and PCL-20% TCP composite scaffolds were stronger (P < 0.05), and the porosity did not change significantly. Pre-designed pore structure could be observed in the PCL, PCL-10%TCP and PCL-20%TCP scaffolds, and the internal pores of the scaffolds were interconnected. Bone marrow mesenchymal stem cells could proliferate on all the three scaffolds. The proliferation rate of the cells on the PCL-20% TCP scaffold was better than that of the other two scaffolds at 4 and 7 days of culture (P < 0.05). The alkaline phosphatase activity (7 and 14 days of culture) and calcium content (14 days of culture) of bone marrow mesenchymal stem cells on the PCL-20% TCP scaffold were significantly higher than those of the PCL and PCL-10%TCP scaffolds (P < 0.05). These results suggest that the PCL-20% TCP scaffold has better mechanical properties, and can promote the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells. 

Key words: Biocompatible Materials, Mesenchymal Stem Cells, Cell Proliferation;, Alkaline Phosphatase, Tissue Engineering

CLC Number: