Biocompatibility of nano fluoridated hydroxyapatite for dental implants

doi:10.3969/j.issn.2095-4344.2014.12.016

Abstract

Abstract:

BACKGROUND: Few reports focus on biocompatibility of nano fluoridated hydroxyapatite for dental implants.
OBJECTIVE: To investigate the biocompatibility in vitro of nano fluoridated hydroxyapatite modified for dental implants.
METHODS: We utilized sol-gel method to prepare nano fluoridated hydroxyapatite and nanohydroxyapatite powders. (1) Hemolysis test: 0.01, 0.15, 0.2 g/L nano fluorinated hydroxyapatite solution, saline and distilled water at a volume of 10 mL were added into 0.2 mL diluted rabbit anti-coagulation blood samples, respectively. Then the supernatant was detected by absorbance values. (2) In vitro cytotoxicity test: Passage 2 L929 cells were respectively cultured in culture media containing 100%, 50% nano fluorinated hydroxyapatite extract, 100% hydroxyapatite extract, phenol solution and RPMI1640. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was employed to measure absorbance values at days 2, 4, 7 of culture.
RESULTS AND CONCLUSION: Hemolysis test in vitro showed the hemolysis rates of nano fluorinated hydroxyapatite groups were less than 5%, which were accorded with haemolysis demand of medical materials. The cytotoxicity test in vitro showed during cultivation, the adherence rate of L929 cells cultured in 100% and 50% nano fluorinated hydroxyapatite extracts were increasing and cell density was rising up. Cells were fusiform or polygon, which had no evident differences from negative controls in morphology. Nano fluorinated hydroxyapatite showed nontoxic to L929 cells in vitro.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: biocompatible materials, hydroxyapatites, dental implants

CLC Number:

R318

You Ying-ying, Feng Yun-zhi . Biocompatibility of nano fluoridated hydroxyapatite for dental implants[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(12): 1901-1906.

Figures/Tables 2

2.1 纳米含氟羟基磷灰石溶血性实验评价 0.01，0.15，0.2 g/L纳米含氟羟基磷灰石组、阳性对照组的A值分别为0.028±0.010，0.046±0.040，0.047±0.020，0.046±0.020，0.797±0.220。0.01，0.15，0.2 g/L纳米含氟羟基磷灰石组的溶血率分别为2.341%，2.471%，2.341%。不同梯度浓度纳米含氟羟基磷灰石的溶血率均在5%以内，表明材料在此浓度范围内符合医用材料的溶血实验要求。 2.2 纳米含氟羟基磷灰石材料体外细胞毒性实验评价(MTT法) 各组细胞培养情况见图2。复合培养第2，4，7天，100%纳米含氟羟基磷灰石浸提液组、50%纳米含氟羟基磷灰石浸提液组、阴性对照组及羟基磷灰石组没有明显区别，细胞形态正常，贴壁生长良好；阳性对照组中几乎为死细胞(图2) 培养第2，4，7天，各组各孔的吸光度值、细胞相对增殖度及细胞毒性分级可见表1。培养第2，4，7天，羟基磷灰石组、100%纳米含氟羟基磷灰石浸提液组、50%纳米含氟羟基磷灰石浸提液组A值与阳性对照组比较差异有显著性意义(P < 0.05)，但与阴性对照组比较差异均无显著性意义(P > 0.05)。同样，羟基磷灰石组、100%纳米含氟羟基磷灰石浸提液组和50%纳米含氟羟基磷灰石浸提液组间差异无显著性意义(P > 0.05)，提示纳米含氟羟基磷灰石材料与传统材料羟基磷灰石相比在体外对L929细胞的增殖无差别。综合分析，培养第2，4，7天纳米含氟羟基磷灰石材料浸提液(100%nFHA、50%nFHA)的细胞相对增殖度均在75%以上，细胞毒性分级为0或1级，说明所制备的纳米含氟羟基磷灰石材料合格，对L929细胞无毒性，符合《医疗器械生物学评价国家标准GB/T16886.5-2003》中对材料细胞毒性要求。 L929细胞生长曲线：以培养时间为横轴，吸光度值为纵轴，绘制L929生长曲线图(图3)。随着培养时间的增加，阴性对照组、羟基磷灰石组、100%纳米含氟羟基磷灰石组、50%纳米含氟羟基磷灰石组L929细胞数目呈增长趋势，生长曲线相近，在第7天细胞数量明显高于第2，4天。阳性对照组在各时间点的细胞数量均少于以上4组，细胞增殖趋势不明显。"

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