Chinese Journal of Tissue Engineering Research ›› 2019, Vol. 23 ›› Issue (6): 930-935.doi: 10.3969/j.issn.2095-4344.1546

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Preparation and in vitro biocompatibility of a novel ternary biomaterial, yttria-stabilized zirconia reinforced nano-hydroxyapatite/polyamide 66

Li Yuling1, Jiang Ke1, Chen Lu1, Yu Peng1, Chen Qian1, Qiao Bo2, Jiang Dianming3   

  1. 1Department of Orthopedics, Affiliated Hospital of North Sichuan Medical College, Nanchong 637000, Sichuan Province, China; 2the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China; 3Bone and Trauma Centre, the Third Affiliated Hospital of Chongqing Medical University, Chongqing 401120, China
  • Received:2018-09-13 Online:2019-02-28 Published:2019-02-28
  • Contact: Jiang Dianming, Professor, Bone and Trauma Centre, the Third Affiliated Hospital of Chongqing Medical University, Chongqing 401120, China
  • About author:Li Yuling, MD, Department of Orthopedics, Affiliated Hospital of North Sichuan Medical College, Nanchong 637000, Sichuan Province, China
  • Supported by:

    the National Natural Science Foundation of China for the Youth, No. NSFC81501876 (to QB); the Applied Basic Project of Science & Technology Department of Sichuan Province, No. 2018JY0250 (to LYL)

Abstract:

BACKGROUND: Nano-hydroxyapatite/polyamide 66 (nHA/PA66) composite materials possess high bionic properties and exert biological activity by directly combining with host bone, but it lacks sufficient mechanical strength.

OBJECTIVE: To prepare a novel ternary biomaterial composed of nHA/PA66/yttria-stabilized tetragonal zirconia (YTZ), and to investigate its mechanical properties and biocompatibility.
METHODS: The biomaterial was prepared by two-step approach, and the mass ratio of nano-hydroxyapatite to yttria-stabilized tetragonal zirconia was 100:0, 90:10, 80:20, and 60:40, respectively. The characterization of nHA/PA66/YTZ was observed by scanning electron microscope. The mechanical parameters of nHA/PA66/YTZ including bending strength, tensile strength, compressive strength, elastic modulus and breaking elongation were tested to evaluate its mechanical properties. The mechanical properties were evaluated by the mechanical tester to select composite materials with the optimum mass ratio applied to the following experiments. The MC3T3-E1 cells were cultured by the cell-culture medium (blank control group), nHA/PA66 material extract (control group), nHA/PA66/YTZ material extract (experimental group). The cell proliferation was detected by cell counting kit-8 assay. The nHA/PA66 material (control group) and nHA/PA66/YTZ material (experimental group) were respectively co-cultured with MC3T3-E1 cells. The adhesion and proliferation of MC3T3-E1 cells on the surface of composite materials were observed by the laser scanning confocal microscope after 24 hours.
RESULTS AND CONCLUSION: The scanning electron microscope showed that YTZ grains filled the gaps between the original nano-hydroxyapatite grains and the nHA/YTZ was evenly dispersed in the matrix of polyamide 66. The biomechanical test revealed that the compressive strength, bending strength, tensile strength, elongation at break and elastic modulus were the highest at 60:40 of the mass ratio of nHA and YTZ, while the mechanical properties were optimal, which could be chosen for the cell compatibility experiments. Cell counting kit-8 assay showed that there was no significant difference in the cell proliferation among groups as the time expended and the number of cells in each group increased. The laser scanning confocal microscope displayed that the cells showed fusion, agglomeration and stratification and more actin filaments in themselves on nHA/PA66/YTZ composite material. The cells had monolayer and dispersion on nHA/PA66 composite material. The number of cells and actin filaments in cells were less than those in the group of nHA/PA66/YTZ. These results showed that the ternary composite materials of nHA/PA66/YTZ exhibit good mechanical properties, biological safety, and biocompatibility in vitro experiment.  

Key words: Hydroxyapatites, Nylons, Materials Testing, Tissue Engineering

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