3D-printed hydroxyapatite/polylactic acid network composites for skull defects

doi:10.3969/j.issn.2095-4344.0676

Abstract

Abstract:

BACKGROUND: Hydroxyapatite (HA)/polylactic acid (PLA) composite has good biocompatibility and osteoinductivity, but few studies have evaluated the feasibility of this composite used as a skull repair material.

OBJECTIVE: To observe the feasibility of 3D-printed HA/PLA network composite in the repair of rabbit skull defects.

METHODS: Twelve New Zealand rabbits were selected and two full-thickness defects of about 1 cm in diameter were prepared on the skull of each rabbit. One of them was implanted with 3D printed HA/PLA network composite and the other was implanted with autologous bone powder. The skull samples were taken at 6 and 12 weeks after implantation for morphological, hardness, and histopathological observations.

RESULTS AND CONCLUSION: (1) At 12 weeks after implantation, the defect profile was observed in the control group, and the defect was basically repaired; the defect in the experimental group was completely filled with new bone tissues and fibrous tissues, and was integrated with the surrounding normal bone tissues. The overall contour was natural and the defect boundary was blurred. Some of the implants were degraded, and the new bones in the inner side of the skull defect converged with the surrounding tissues. (2) At 6 weeks after implantation, the microhardness of the repaired area in the experimental group was lower than that in the control group (P < 0.05), both of which were lower than the hardness of normal bone (P < 0.05). At 12 weeks after implantation, the microhardness of the repaired area in the experimental group showed no difference from that of the control group and the normal group. (3) At 6 weeks after implantation, the bone trabeculae formed well in the experimental group and were interconnected partly, most of which extended along the skull defect in an orderly manner. Moreover, neovascularization and bone marrow cavity formed. In the control group, the thickness of trabecular bone was better but the alignment was irregular. At 12 weeks after implantation, dense bone trabeculae formed in the experimental group with good connectivity. There were bone cells and trabecular bones in the implants. In the control group, the thickness of bone trabeculae was good, but the alignment was irregular. Blood vessels and bone units could be seen, and the marrow cavity formed well. Overall, these findings suggest that the 3D-printed HA/PLA network composite has good biocompatibility, osteoinduction, and biodegradability, which can be used for skull repair effectively.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Hydroxyapatites, Compomers, Skull, Tissue Engineering

CLC Number:

Liu Dong, Qin Hu, Wang Yongxin, Li Yabin, Gao Yong, Fan Guofeng, Wang Zengliang. 3D-printed hydroxyapatite/polylactic acid network composites for skull defects[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(6): 833-837.

Figures/Tables 3

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