Cell affinity of cartilage tissue engineering scaffolds prepared by poly(hydroxybutyrate-co-hydroxyoctanoate)/collagen composite materials

doi:10.3969/j.issn.2095-4344.2014.12.015

Abstract

Abstract:

BACKGROUND: Many experiments have demonstrated that tissue engineering scaffolds prepared by polymer materials alone or biomaterials cannot meet the requirement of tissue engineering research.
OBJECTIVE: To evaluate biological characteristics and cell affinity of poly(hydroxybutyrate-co-hydroxyoctanoate)/collagen composite scaffold.
METHODS: Tissue engineering scaffolds were prepared by combination of poly(hydroxybutyrate-co-hydroxyoctanoate) and collagen at different proportions (2%, 4%, 6%, 8% and 10%) using solvent casting/particulate leaching method. Inner structure and apertures were observed by scanning electron microscope, and the porosity was determined by liquid displacement method. Rabbit chondrocytes were co-cultured with poly(hydroxybutyrate-co-hydroxyoctanoate)/collagen composite scaffold and poly(hydroxybutyrate-co-hydroxyoctanoate) scaffold. Growth curve of cells was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, and cell adhesion on the scaffolds was observed by scanning electron microscope.
RESULTS AND CONCLUSION: The pore size and porosity of the composite scaffold were about 200 μm and (85±2)%, respectively. The cell affinity dynamically increased with the increasing of proportion of collagen. Compared with the poly(hydroxybutyrate-co-hydroxyoctanoate) scaffold, the poly(hydroxybutyrate-co- hydroxyoctanoate)/collagen composite scaffolds are better to improve cell adhesion and proliferation, with favorable cellular affinity.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: biocompatible materials, 3-hydroxybutyric acid, collagen, chondrocytes

CLC Number:

R318

Lu Xing, Zhang Yong-hong, Li Er-feng, Wang Zeng-rong, Zhao Liang-qi . Cell affinity of cartilage tissue engineering scaffolds prepared by poly(hydroxybutyrate-co-hydroxyoctanoate)/collagen composite materials[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(12): 1895-1900.

Figures/Tables 2

References

[1] 陈竹生,吕玉明,张兵.纳米β-磷酸三钙/Ⅰ、Ⅱ型胶原层状支架复合骨髓间充质干细胞修复膝关节骨软骨缺损[J].中国组织工程研究与临床康复,2010,14(42):7797-7801.

[2] Kerker JT,Leo AJ,Sgaglione NA.Cartilage repair: synthetics and scaffolds: basic science, surgical techniques,and clinical outcomes.Sports Med Arthrosc.2008;16(4):208-216.

[3] 董岳峰,谢红,郭羽,等.新型支架材料羟基丁酸与羟基辛酸共聚体的生物相容性[J].中国组织工程研究与临床康复,2008,12(10): 1870-1872.

[4] 张蝉,董岳峰,王海宾,等.费氏中华根瘤菌合成羟基丁酸和羟基己酸共聚体的研究[J].微生物学通报,2007,34(6):1077-1081.

[5] 杜江华,杨青芳,张楠楠,等.聚羟基丁酸酯支架在组织工程中的应用[J].中国组织工程研究与临床康复,2006,10(37):114-116.

[6] 吴俊,孙俊英,李海燕,等.以PHBV为支架构建组织工程化软骨[J].中国矫形外科杂志,2006,14(13):1016-1018.

[7] 张玲,敖强,贺庆,等.聚羟基丁酸己酸酯/聚碳酸亚丙酯共混材料作为血管组织工程支架的性能特征[J].中国组织工程研究与临床康复,2007,11(35):6950-6953.

[8] Lee CH,Singla A,Lee Y.Biomedical applications of collagen.Int J Pharm.2001;221:1-2.

[9] Temenoff JS,Mikos AG.Review: tissue engineering forregeneration of articular cartilage.Biomaterials. 2000;21(5): 431-440.

[10] 张永红,许钰,林汲,等.羟基丁酸-羟基辛酸共聚体/脱细胞软骨基质支架的细胞黏附性[J].中国组织工程研究与临床康复,2010, 14(25):4585-4588.

[11] 朱艳霞,宋克东,文鹏飞,等.体外培养脂肪组织来源干细胞与胶原/壳聚糖复合支架的亲和性[J].中国组织工程研究与临床康复, 2007,11(31):6155-6160.

[12] 徐成峰,胡大海,赵周婷,等.兔骨髓间充质干细胞体外分离培养及多向诱导分化[J].中国组织工程研究与临床康复,2010,14(6): 1002-1005.

[13] 许圣荣,赵劲民,苏伟.骨髓间充质干细胞向软骨细胞分化的实验研究[J].广西医科大学学报,2010,27(5):657-660.

[14] 张厚安,李敏,唐思文.骨组织工程用细胞支架生物材料的研究进展[J].机械工程材料,2007,31(12):4-7.

[15] 秦胜男,陈鸿辉.胶原在软骨组织工程中的应用[J].中国矫形外科杂志,2009,17(13):998-1000.

[16] 李二峰,王增荣,陆兴,等.羟基丁酸-羟基辛酸聚合物-胶原骨软骨支架的制备及应用[J].中国组织工程研究,2012,16(47): 8751-8754.

[17] 刘耀升,刘蜀彬,李鼎锋.软骨组织工程研究中的刺激因子[J].中国组织工程研究与临床康复,2009,13(15):2951-2954.

[18] 初殿伟,王玮,陈立军,等.兔骨髓间充质干细胞/壳聚糖-胶原支架复合体构建软骨组织工程支架[J].中国组织工程研究与临床康复,2009,13(42):8248-8252.

[19] Kramer J,Hegert C,Guan K,et al.Embryonic stem cells derived chondrogenic differentiation in vitro: activation by BMP 22 and BMP 24.Mech Dev.2000;92:193.

[20] 林荔军,李奇,靳安民,等.胶原酶消化分离关节软骨种子细胞的优化获取[J].中华创伤骨科杂志,2004,6(2):760-762.