Chinese Journal of Tissue Engineering Research ›› 2024, Vol. 28 ›› Issue (31): 4980-4986.doi: 10.12307/2023.771
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Ma Dong1, Chen Qiqing2, Zhao Jirong1, Huang Junkai1, Yang Yunyun1, Zhu Bao2, Zhao Ning2, Ma Tong2
Received:
2022-10-26
Accepted:
2023-01-04
Online:
2024-11-08
Published:
2024-01-22
Contact:
Chen Qiqing, MD, Associate chief physician, Gansu Provincial Hospital of TCM, Lanzhou 730050, Gansu Province, China
About author:
Ma Dong, Master candidate, Gansu University of Chinese Medicine, Lanzhou 730030, Gansu Province, China
Supported by:
CLC Number:
Ma Dong, Chen Qiqing, Zhao Jirong, Huang Junkai, Yang Yunyun, Zhu Bao, Zhao Ning, Ma Tong. Isolation, purification and identification of rat annulus fibrosus-derived stem cells by adherent method combined with fibronectin differential adhesion method[J]. Chinese Journal of Tissue Engineering Research, 2024, 28(31): 4980-4986.
2.7 AFSCs诱导前后基因检测结果 成骨诱导培养3周后,对AFSCs进行成骨细胞特征性基因检测,结果显示Collagen-Ⅰ、 Runx2 基因在细胞中呈高表达,而未诱导组无明显表达,差异有显著性意义(P < 0.05),见图9A;成软骨诱导培养3周后,对AFSCs进行成软骨细胞特征性基因检测,结果显示Collagen-Ⅱ、Sox-9 基因在细胞中呈高表达,而未诱导组无明显表达,差异有显著性意义(P < 0.05),见图9B;成脂诱导培养2 周后,对AFSCs进行脂肪细胞特征性基因检测,结果显示,PPAR-γ、LPL 基因在细胞中呈高表达,而未诱导组无明显表达,差异有显著性意义(P < 0.05),见图9C。"
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