Chinese Journal of Tissue Engineering Research ›› 2020, Vol. 24 ›› Issue (13): 2011-2019.doi: 10.3969/j.issn.2095-4344.2064
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Qin Danying1, Zhang Yuheng1, Li Xueyong1, Yang Wenxian2
Received:
2019-10-16
Revised:
2019-10-21
Accepted:
2019-11-25
Online:
2020-05-08
Published:
2020-03-07
Contact:
Li Xueyong, MD, Chief physician, Department of Plastic Surgery and Burn, Second Affiliated Hospital of Air Force Military Medical University (Tangdu Hospital), Xi’an 710000, Shaanxi Province, China
Yang Wenxian, Master, Attending physician, Department of Plastic Surgery, Second People’s Hospital of Wuxi, Wuxi 214002, Jiangsu Province, China
About author:
Qin Danying, MD candidate, Attending physician, Department of Plastic Surgery and Burn, Second Affiliated Hospital of Air Force Military Medical University (Tangdu Hospital), Xi’an 710000, Shaanxi Province, China
Supported by:
CLC Number:
Qin Danying, Zhang Yuheng, Li Xueyong, Yang Wenxian. Proteomics analysis of exosomes from adipose-derived stem cells in skin damage repair[J]. Chinese Journal of Tissue Engineering Research, 2020, 24(13): 2011-2019.
2.1.4 外泌体纳米微粒追踪检测结果 样本粒径分布主要集中在30-200 nm,样本的粒子浓度为1014 L-1,样本3次重复测定的结果较为一致,说明外泌体的粒径大小整体符合标准,粒径分布较为均一。25例患者之间、10名健康人之间外泌体浓度差异无显著性意义;25例患者外泌体浓度平均值为0.70×1014 L-1,10名健康人外泌体浓度为1.14×1014 L-1,两组比较差异有显著性意义(P < 0.05)。因此得出结论:第一,血糖及软组织缺损面积的差异并不影响脂肪干细胞来源外泌体的浓度;第二,有理由相信两组在蛋白组成等方面具有差异性,此为进一步实验的基础。分别从25例患者样本及10例健康人中各随机抽取3例样本行差异蛋白提取,抽取的样本编号为A7、A11、A23;Z5、Z8、Z9。粒径以及粒子数浓度见表2。粒子的总体分布,见图5。 "
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