Adiponectin levels in plasma and periodontal tissue of mouse models of diabetic periodontitis treated by adiponectin

doi:10.3969/j.issn.2095-4344.1315

Abstract

Abstract:

BACKGROUND: Low level of adiponectin plays a significant role in the occurrence and development of periodontitis and diabetes mellitus.
OBJECTIVE: To investigate the therapeutic effect of adiponectin injection on diabetic periodontitis model mice and its mechanism.
METHODS: Sixty 8-week old Kunming mice (provided by Beijing Weitong Lihua Animal Experimental Technology Co., Ltd.) were randomly divided into three groups: control group, model group and adiponectin group. The mice in the model and adiponectin groups received intraperitoneal injection of streptozotin to establish a mouse model of diabetes mellitus, and then the periodontitis model was established by applying the combined bacterial fluid to the ligation site. The mice in the control and model groups received intraperitoneal injection of 1 mL normal saline once daily, while adiponectin group received intraperitoneal injection of 15 μg/kg adiponectin once daily, for 6 consecutive weeks. The three experimental groups of mice were treated for 6 weeks, after which physiological and biochemical indicators were tested. The levels of adiponectin in serum and periodontal tissue, the blood sugar and plasma insulin levels were then detected by ELISA. The depth of periodontal examination and the vertical absorption of alveolar bone were observed. The pathological changes of periodontal tissue were detected by hematoxylin-eosin staining. The changes of apoptosis level of gingival epithelial cells were detected by TUNEL method. The expression levels of RAGE and reactive oxygen species mRNA and protein were detected by RT-PCR and western blot assay.
RESULTS AND CONCLUSION: (1) The levels of adiponectin in serum and periodontal tissue in the adiponectin group were significantly higher than those in the control and model groups (P < 0.05). (2) The blood sugar level in the adiponectin group was significantly lower than that in the model group (P < 0.05) and the serum insulin level was significantly increased (P < 0.05). (3) The depth of periodontal examination and vertical absorption of alveolar bone were decreased significantly in the adiponectin group compared with the model group, and the apoptotic rate of gingival epithelial cells was decreased significantly. (4) The expression levels of RAGE and reactive oxygen species mRNA and protein in the adiponectin group were significantly lower than those in the model group (P < 0.05). (5) These results suggest that adiponectin can improve the adiponectin level in periodontal tissue and plasma in the diabetic periodontitis model mice and can significantly improve the blood sugar status, and reduce the pathological changes of periodontal tissue and the apoptosis of periodontal cells possibly via regulating RAGE-ROS signaling pathway.

Key words: adiponectin, diabetes mellitus, periodontitis, RAGE-ROS signaling pathway, periodontal tissue, periodontal cells, apoptosis, tissue construction

CLC Number:

R496
R318

Yang Na, Bao Pingping, Lei Tao. Adiponectin levels in plasma and periodontal tissue of mouse models of diabetic periodontitis treated by adiponectin [J]. Chinese Journal of Tissue Engineering Research, 2019, 23(23): 3692-3697.

Figures/Tables 6

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