Effect of umbilical cord blood mesenchymal stem cell transplantation on myocardial cell apoptosis

doi:10.3969/j.issn.2095-4344.2016.14.022

Abstract

Abstract:

BACKGROUND: Umbilical cord blood mesenchymal stem cell transplantation can reduce myocardial apoptosis and myocardial fibrosis, thereby improving the cardiac function.

OBJECTIVE: To observe the effect of umbilical cord blood mesenchymal stem cell transplantation on myocardial apoptosis in rats.

METHODS: (1) In vitro test: Normal myocardial cells cultured for 72 hours were enrolled as control group. Myocardial cells injured by adriamycin alone were enrolled as injury group. Adriamycin-injured myocardial cells were co-cultured with umbilical cord blood mesenchymal stem cells as co-culture group. After 48 hours of culture, TUNEL assay was used to assess myocardial apoptosis in rats. (2) In vivo test: Forty-five Sprague-Dawley rats were randomized into normal control, adriamycin, and umbilical cord blood mesenchymal stem cell transplantation (cell transplantation) groups. Two weeks after cell transplantation, PowerLab was used to test cardiac function, and western blot employed to detect Bax and Caspase-3 expression levels. Meanwhile, pathological changes of the myocardial tissues were observed in the three groups.
RESULTS AND CONCLUSION: (1) In vitro test: The apoptotic rate of myocardial cells was significantly increased in the injury group compared with the control group, but decreased significantly in the co-culture group compared with the injury group (P < 0.05). (2) In vivo test: In the cell transplantation group, the cardiac function indexes, expression levels of Bax and Caspase-3 and myocardial infarction size were significantly improved compared with the adriamycin group (P < 0.05), but still not recovered to the normal levels (P < 0.05). These results show that umbilical cord blood mesenchymal stem cells acting on the rat myocardial cells can inhibit adriamycin-induced myocardial apoptosis, and the relevant mechanism is probably related to down-regulation of Bax and caspase-3 expression.
中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Cord Blood Stem Cell Transplantation, Myocytes, Cardiac, Apoptosis, Tissue Engineering

Wang Yan-li, Li Jin-feng, Wang Yan-ping, Gao Ming. Effect of umbilical cord blood mesenchymal stem cell transplantation on myocardial cell apoptosis[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(14): 2123-2128.

Figures/Tables 7

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