Chinese Journal of Tissue Engineering Research ›› 2023, Vol. 27 ›› Issue (33): 5249-5255.doi: 10.12307/2023.750
Tang Li, Pan Yao, Zhu Guochen
Received:
2022-10-24
Accepted:
2022-12-09
Online:
2023-11-28
Published:
2023-03-29
Contact:
Zhu Guochen, MD, Chief physician, Professor, Master’s supervisor, Department of Otorhinolaryngology-Head and Neck Surgery, Affiliated Wuxi No. 2 People’s Hospital, Nanjing Medical University, Wuxi 214002, Jiangsu Province, China
About author:
Tang Li, Master candidate, Department of Otorhinolaryngology-Head and Neck Surgery, Affiliated Wuxi No. 2 People’s Hospital, Nanjing Medical University, Wuxi 214002, Jiangsu Province, China
Supported by:
CLC Number:
Tang Li, Pan Yao, Zhu Guochen. Epidermal neural crest stem cells of hair follicles regulate the local expression of inflammatory factors after facial nerve injury[J]. Chinese Journal of Tissue Engineering Research, 2023, 27(33): 5249-5255.
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2.2 实验动物一般情况分析 实验选用SD大鼠 54 只,各时间节点所有大鼠均存活,一般状况可,无局部伤口感染,日常行为状态、活跃程度、饮食、睡眠、精神状态等均未见明显异常,排除躯体卷曲、震颤等颅内感染症状。 2.3 EPI-NCSCs 调节了肿瘤坏死因子α和白细胞介素4的表达水平 2.3.1 免疫蛋白印迹检测结果 桥接后3,7,14 d测定相关细胞因子表达,以观察局部炎症微环境的变化。蛋白质印迹检测促炎细胞因子肿瘤坏死因子α和抗炎细胞因子白细胞介素4的表达水平如图4所示。在术后7 d,与生理盐水组和DMEM组相比,EPI-NCSCs 组的肿瘤坏死因子α表达水平显著降低(P < 0.05),而在术后3,14 d时未见显著性差异(P > 0.05);生理盐水组和DMEM组间比较在术后3,7,14 d都未见显著性差异(P > 0.05)。在术后3,7,14 d,EPI-NCSCs 组的白细胞介素4表达较生理盐水组和DMEM 组显著增高(P < 0.05),而生理盐水组和DMEM组间比较未见显著性差异(P > 0.05)。"
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