Chinese Journal of Tissue Engineering Research ›› 2023, Vol. 27 ›› Issue (16): 2518-2524.doi: 10.12307/2023.168
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Xia Yu1, 2, Sun Jia2, Qi Zhengyan1, 2, Ma Lin1, 2, Ma Wenqian2, Niu Jianguo1, 2, Wen Yujun1, 2
Received:
2022-04-09
Accepted:
2022-06-08
Online:
2023-06-08
Published:
2022-11-10
Contact:
Wen Yujun, Professor, Master’s supervisor, Department of Human Anatomy and Histology Embryology, School of Basic Medicine, Ningxia Medical University, Yinchuan 750004, Ningxia Hui Autonomous Region, China; Ningxia Key Laboratory of Craniocerebral Diseases, Ningxia Medical University, Yinchuan 750004, Ningxia Hui Autonomous Region, China
About author:
Xia Yu, Master candidate, Department of Human Anatomy and Histology Embryology, School of Basic Medicine, Ningxia Medical University, Yinchuan 750004, Ningxia Hui Autonomous Region, China; Ningxia Key Laboratory of Craniocerebral Diseases, Ningxia Medical University, Yinchuan 750004, Ningxia Hui Autonomous Region, China
Supported by:
CLC Number:
Xia Yu, Sun Jia, Qi Zhengyan, Ma Lin, Ma Wenqian, Niu Jianguo, Wen Yujun. Repair effect of poly(lactic-co-glycolic acid) nanospheres with sustained-release of neurotrophin-3 and ganglioside GD1a on spinal cord injury in rats[J]. Chinese Journal of Tissue Engineering Research, 2023, 27(16): 2518-2524.
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2.2 各组大鼠后肢运动功能恢复情况 所有大鼠术前BBB评分均为满分,术后无意外死亡。除假手术组外,其余4组大鼠术后1 d均呈截瘫状态,后肢运动功能丧失,靠前肢爬行,BBB评分为0分;从术后2周开始,大鼠后肢运动功能开始恢复,神经营养因子组、实验组大鼠术后第7,8周的BBB评分高于脊髓损伤组(P < 0.05),神经节苷脂组大鼠术后的BBB评分与脊髓损伤组比较差异无显著性意义(P > 0.05),见图2A。 旷场实验结果显示,神经营养因子组、实验组大鼠术后第7,8周的旷场移动总距离大于脊髓损伤组(P < 0.05),神经节苷脂组大鼠术后的旷场移动总距离与脊髓损伤组比较差异无显著性意义(P > 0.05),见图2B。"
2.4 各组大鼠脊髓损伤区尾侧白质腹侧神经纤维NF表达 免疫荧光染色显示,与假手术组相比,其他4组大鼠脊髓损伤区尾侧白质腹侧神经纤维NF表达均不同程度地减少;术后4周,实验组大鼠脊髓损伤区尾侧脊髓白质腹侧神经纤维NF表达多于脊髓损伤组(P < 0.05),神经营养因子组、神经节苷脂组大鼠脊髓损伤区尾侧脊髓白质腹侧神经纤维NF表达与脊髓损伤组比较差异无显著性意义(P > 0.05)。术后8周,神经营养因子组、实验组大鼠脊髓损伤区尾侧脊髓白质腹侧神经纤维NF表达多于脊髓损伤组(P < 0.05),神经节苷脂组大鼠脊髓损伤区尾侧脊髓白质腹侧神经纤维NF表达与脊髓损伤组比较差异无显著性意义(P > 0.05),见图4。"
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