Chinese Journal of Tissue Engineering Research ›› 2023, Vol. 27 ›› Issue (8): 1193-1198.doi: 10.12307/2023.138
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Long Guiyue1, Li Dongdong1, Liao Hongbing2
Received:
2022-03-09
Accepted:
2022-05-14
Online:
2023-03-18
Published:
2022-07-27
Contact:
Liao Hongbing, MD, Professor, Department of Prosthetics, School of Stomatology, Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China
About author:
Long Guiyue, Master candidate, Guangxi Key Laboratory of Oral and Maxillofacial Restoration and Reconstruction, Guangxi Cranio-Maxillofacial Malformation Clinical Research Center, Guangxi Key Laboratory of Oral and Maxillofacial Surgery, Medical Experimental Center of Guangxi Medical University, School of Stomatology, Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China
Supported by:
CLC Number:
Long Guiyue, Li Dongdong, Liao Hongbing. Calcium phosphate cement/poly(lactic-co-glycolic acid) degradation products promote osteoclast differentiation of mouse monocytes[J]. Chinese Journal of Tissue Engineering Research, 2023, 27(8): 1193-1198.
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各组细胞经过48 h潜伏后进入对数生长期,48 h时,CPC/PLGA组细胞数比对照组多,差异有显著性意义(P < 0.05);72 h时,CPC/PLGA组的细胞数明显高于对照组及羟基乙酸组,差异有显著性意义(P < 0.05);72 h后,细胞增长速率减慢;在96 h时,细胞数量达到顶峰;120 h时,各组细胞数量下降,CPC/PLGA组较其他两组显著降低(P < 0.001)。 2.2 RT-PCR检测结果 CPC/PLGA组NFATc1、核因子κB受体活化因子、抗酒石酸酸性磷酸酶 mRNA的表达水平较对照组和羟基乙酸组升高,差异有显著性意义(P < 0.01);羟基乙酸组和对照组的NFATc1、核因子κB受体活化因子、抗酒石酸酸性磷酸酶的mRNA表达无差异;羟基乙酸组、CPC/PLGA组的金属基质蛋白酶9 mRNA表达水平显著高于对照组,差异有显著性意义(P < 0.001),见图2。 "
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